Biofabrication of SDF-1 functionalized 3D-printed cell-free scaffolds for bone tissue regeneration
- Large segmental bone defects occurring after trauma, bone tumors, infections or revision surgeries are a challenge for surgeons. The aim of our study was to develop a new biomaterial utilizing simple and cheap 3D-printing techniques. A porous polylactide (PLA) cylinder was printed and functionalized with stromal-derived factor 1 (SDF-1) or bone morphogenetic protein 7 (BMP-7) immobilized in collagen type I. Biomechanical testing proved biomechanical stability and the scaffolds were implanted into a 6 mm critical size defect in rat femur. Bone growth was observed via x-ray and after 8 weeks, bone regeneration was analyzed with µCT and histological staining methods. Development of non-unions was detected in the control group with no implant. Implantation of PLA cylinder alone resulted in a slight but not significant osteoconductive effect, which was more pronounced in the group where the PLA cylinder was loaded with collagen type I. Addition of SDF-1 resulted in an osteoinductive effect, with stronger new bone formation. BMP-7 treatment showed the most distinct effect on bone regeneration. However, histological analyses revealed that newly formed bone in the BMP-7 group displayed a holey structure. Our results confirm the osteoinductive character of this 3D-biofabricated cell-free new biomaterial and raise new options for its application in bone tissue regeneration.
Author: | Alina Lauer, Philipp Wolf, Dorothea Mehler, Hermann Götz, Mehmet Rüzgar, Andreas Baranowski, Dirk HenrichORCiDGND, Pol Maria Rommens, Ulrike Ritz |
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URN: | urn:nbn:de:hebis:30:3-544607 |
DOI: | https://doi.org/10.3390/ijms21062175 |
Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/32245268 |
Parent Title (English): | International journal of molecular sciences |
Publisher: | MDPI |
Place of publication: | Basel |
Document Type: | Article |
Language: | English |
Date of Publication (online): | 2020/03/21 |
Date of first Publication: | 2020/03/21 |
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Release Date: | 2020/04/09 |
Tag: | 3D printed cell-free scaffold; bone tissue regeneration; collagen type I; in vivo model of critical size defects; polylactide; stromal-derived factor 1 |
Volume: | 21 |
Issue: | 2175 |
Page Number: | 17 |
Note: | This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited |
HeBIS-PPN: | 463907469 |
Institutes: | Medizin / Medizin |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Sammlungen: | Universitätspublikationen |
Licence (German): | Creative Commons - Namensnennung 4.0 |