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We discuss gapless colour superconductivity for neutral quark matter in β equilibrium at zero as well as at nonzero temperature. Basic properties of gapless superconductors are reviewed. The current progress and the remaining problems in the understanding of the phase diagram of strange quark matter are discussed.
At present, there are no quantitative, objective methods for diagnosing the Parkinson disease. Existing methods of quantitative analysis by myograms suffer by inaccuracy and patient strain; electronic tablet analysis is limited to the visible drawing, not including the writing forces and hand movements. In our paper we show how handwriting analysis can be obtained by a new electronic pen and new features of the recorded signals. This gives good results for diagnostics. Keywords: Parkinson diagnosis, electronic pen, automatic handwriting analysis
Topological semimetal antiferromagnets provide a rich source of exotic topological states which can be controlled by manipulating the orientation of the Néel vector, or by modulating the lattice parameters through strain. We investigate via ab initio density functional theory calculations, the effects of shear strain on the bulk and surface states n two antiferromagnetic EuCd2As2 phases with out-of-plane and in-plane spin configurations. When magnetic moments are along the c-axis, a 3% longitudinal or diagonal shear strain can tune the Dirac semimetal phase to an axion insulator phase, characterized by the parity-based invariant η4I=2. For an in-plane magnetic order, the axion insulator phase remains robust under all shear strains. We further find that for both magnetic orders, the bulk gap increases and a surface gap opens on the (001) surface up to 16 meV. Because of a nonzero η4I index and gapped states on the (001) surface, hinge modes are expected to happen on the side surface states between those gapped surface states. This result can provide a valuable insight in the realization of the long-sought axion states.
We study the phase diagram of a generalized chiral SU(3)-flavor model in mean-field approxi- mation. In particular, the influence of the baryon resonances, and their couplings to the scalar and vector fields, on the characteristics of the chiral phase transition as a function of temperature and baryon-chemical potential is investigated. Present and future finite-density lattice calculations might constrain the couplings of the fields to the baryons. The results are compared to recent lattice QCD calculations and it is shown that it is non-trivial to obtain, simultaneously, stable cold nuclear matter.
The space-time dynamics and pion-HBT radii in central heavy ion-collisions at CERN-SPS and BNL-RHIC are investigated within a hydrodynamic simulation. The dependence of the dynamics and the HBT-parameters on the EoS is studied with different parametrizations of a chiral SU(3) sigma omega model. The selfconsistent collective expansion includes the e ects of e ective hadron masses, generated by the nonstrange and strange scalar condensates. Different chiral EoS show di erent types of phase transitions and even a crossover. The influence of the order of the phase transition and of the latent heat on the space-time dynamics and pion-HBT radii is studied. A small latent heat, i.e. a weak first-order chiral phase transition, or a smooth crossover lead to distinctly di erent HBT predictions than a strong first order phase transition. A quantitative description of the data, both at SPS energies as well as at RHIC energies, appears di cult to achieve within the ideal hydrodynamic approach using the SU(3) chiral EoS. A strong first-order quasi-adiabatic chiral phase transition seems to be disfavored by the pion-HBT data from CERN-SPS and BNL-RHIC.
The measured particle ratios in central heavy-ion collisions at RHIC-BNL are investigated within a chemical and thermal equilibrium chiral SU(3) theta - omega approach. The commonly adopted noninteracting gas calculations yield temperatures close to or above the critical temperature for the chiral phase transition, but without taking into account any interactions. Contrary, the chiral SU(3) model predicts temperature and density dependent e ective hadron masses and e ective chemical potentials in the medium and a transition to a chirally restored phase at high temperatures or chemical potentials. Three di erent parametrizations of the model, which show di erent types of phase transition behaviour, are investigated. We show that if a chiral phase transition occured in those collisions, freezing of the relative hadron abundances in the symmetric phase is excluded by the data. Therefore, either very rapid chemical equilibration must occur in the broken phase, or the measured hadron ratios are the outcome of the dynamical symmetry breaking. Furthermore, the extracted chemical freeze-out parameters di er considerably from those obtained in simple noninteracting gas calculations. In particular, the three models yield up to 35 MeV lower temperatures than the free gas approximation. The in-medium masses turn out di er up to 150 MeV from their vacuum values.
A nonlinear chiral SU(3) approach including the spin 3 2 decuplet is developed to describe dense matter. The coupling constants of the baryon resonances to the scalar mesons are determined from the decuplet vacuum masses and SU(3) symmetry relations. Di erent methods of mass generation show significant differences in the properties of the spin- 3 2 particles and in the nuclear equation of state
Introduction: Until now it is not possible to determine the equation of state (EOS) of hadronic matter from QCD. One succesfully applied alternative way to describe the hadronic world at high densities and temperatures are effective models like the RMF-models [1], where the relevant degrees of freedom are baryons and mesons instead of quarks and gluons. Since approximate chiral symmetry is an essential feature of QCD, it should be a useful concept for building and restricting e ective models. It has been shown [2,3] that effective sigma-omega models including SU(2) chiral symmetry are able to obtain a reasonable description of nuclear matter and finite nuclei. Recently [4] we have shown that an extended SU(3) × SU(3) chiral sigma-omega model is able to describe nuclear matter ground state properties, vacuum properties and finite nuclei satisfactorily. This model includes the lowest SU(3) multiplets of the baryons (octet and decuplet[5]), the spin-0 and the spin-1 mesons as the relevant degrees of freedom. Here we will discuss the predictions of this model for dense, hot, and strange hadronic matter.
A significant drop of the vector meson masses in nuclear matter is observed in a chiral SU(3) model due to the e ects of the baryon Dirac sea. This is taken into account through the summation of baryonic tadpole diagrams in the relativistic Hartree approximation. The appreciable decrease of the in-medium vector meson masses is due to the vacuum polarisation e ects from the nucleon sector and is not observed in the mean field approximation.
Compelling evidence for a new form of matter has been claimed to be formed in Pb+Pb collisions at SPS. We critically review two suggested signatures for this new state of matter: First the suppression of the J/psi , which should be strongly suppressed in the QGP by two different mechanisms, the color-screening [1] and the QCD-photoe ect [2]. Secondly the measured particle, in particular strange hadronic, ratios might signal the freeze-out from a quark-gluon phase.
Compelling evidence for the creation of a new form of matter has been claimed to be found in Pb+Pb collisions at SPS. We discuss the uniqueness of often proposed experimental signatures for quark matter formation in relativistic heavy ion collisions. It is demonstrated that so far none of the proposed signals like J/psi meson production/suppression, strangeness enhancement, dileptons, and directed flow unambigiously show that a phase of deconfined matter has been formed in SPS Pb+Pb collisions. We emphasize the need for systematic future measurements to search for simultaneous irregularities in the excitation functions of several observables in order to come close to pinning the properties of hot, dense QCD matter from data.
The interaction of Eph receptor tyrosine kinases with their transmembrane ligands; the ephrins, is important for the regulation of cell-cell communication. Ephrin-Eph signaling is probably best known for the discrimination of arterial and venous territories by repulsion of venous endothelial cells away from those with an arterial fate. Ultimately, cell repulsion is mediated by initiating the collapse of the actin cytoskeleton in membrane protrusions. Here, we investigated the role of the Ena/VASP family of actin binding proteins in endothelial cell repulsion initiated by ephrin ligands. Human endothelial cells dynamically extended sheet-like lamellipodia over ephrin-B2 coated surfaces. While lamellipodia of control siRNA transfected cells rapidly collapsed, resulting in a pronounced cell repulsion from the ephrin-B2 surfaces, the knockdown of Ena/VASP proteins impaired the cytoskeletal collapse of membrane protrusions and the cells no longer avoided the repulsive surfaces. Mechanistically, ephrin-B2 stimulation elicited the EphB-mediated tyrosine phosphorylation of VASP, which abrogated its interaction with the focal adhesion protein Zyxin. Nck2 was identified as a novel VASP binding protein, which only interacted with the tyrosine phosphorylated VASP protein. Nck links Eph-receptors to the actin cytoskeleton. Therefore, we hypothesize that Nck-Ena/VASP complex formation is required for actin reorganization and/or Eph receptor internalization downstream of ephrin-Eph interaction in endothelial cells, with implications for endothelial navigation and pathfinding.
Endothelial tip cells are essential for VEGF-induced angiogenesis, but underlying mechanisms are elusive. Endothelial-specific deletion of EVL, a member of the mammalian Ena/VASP protein family, reduced the expression of the tip cell marker protein endothelial cell specific molecule-1 (Esm1) and compromised the radial sprouting of the vascular plexus in the postnatal mouse retina. The latter effects could at least partly be attributed to reduced VEGF receptor 2 (VEGFR2) internalization and signaling but the underlying mechanisms(s) are not fully understood. In the present study, we revealed that the expression of the long non-coding RNA H19 was significantly reduced in endothelial cells from postnatal EVL-/- mice and in siRNA-transfected human endothelial cells under hypoxic conditions. H19 was recently shown to promote VEGF expression and bioavailability via Esm1 and hypoxia inducible factor 1α (HIF-1α). Similar to EVL-/- mice, the radial outgrowth of the vascular plexus was significantly delayed in the postnatal retina of H19-/- mice. In summary, our data suggests that loss of EVL not only impairs VEGFR2 internalition and downstream signaling, but also impairs VEGF expression and bioavailability in the hypoxic retina via downregulation of lncRNA H19.
In der Abteilung Grammatik des Instituts für Deutsche Sprache, Mannheim, wird derzeit ein neues Projekt entwickelt, und zwar das einer Grammatik des Deutschen im europäischen Vergleich (GDE). Dieses Projekt fügt sich ein in die kontrastive Tradition des IDS, ist jedoch andererseits auch in vieler Hinsicht innovativ. Bevor ich das Projekt im Einzelnen vorstelle, versuche ich den Bogen zurück zu den kontrastiven Grammatiken zu schlagen. Gerade die Leserschaft polnischer Germanisten braucht an die Tradition kontrastiver Grammatikschreibung sicher nicht eigens erinnert zu werden. Denn diese Tradition, die untrennbar mit dem Namen Ulrich Engel verknüpft ist, ist gerade erst in der neu erschienenen deutsch-polnischen kontrastiven Grammatik kulminiert. Im Bereich der kontrastiven Grammatiken zu Sprachenpaaren, von denen das Deutsche ein Element ist, verfügt das IDS also über eine vergleichsweise reiche Tradition. Am IDS oder in Kooperation mit dem IDS wurden kontrastive Grammatiken zu den Sprachenpaaren Deutsch – Französisch (Zemb 1978), Deutsch – Serbokroatisch , Deutsch – Spanisch (Cartegena/Gauger 1989), Deutsch – Rumänisch (Engel u.a. 1993) erarbeitet. Zum Sprachenpaar Englisch – Deutsch liegt mit Hawkins 1986 eine typologisch-vergleichende Grammatik vor. Die deutsch-polnische kontrastive Grammatik, die unter der Leitung von Ulrich Engel erarbeitet wurde, ist 1999 erscheinen. Abraham 1994 und Glinz 1994 konfrontieren das Deutsche, mit durchaus unterschiedlicher Akzentsetzung, mit mehreren anderen europäischen Sprachen. An der Berliner Humboldt-Universität laufen derzeit die Vorarbeiten zu einer deutsch-russischen kontrastiven Grammatik (Initiative Wolfgang Gladrow und Michail Kotin). Die Aufgabe einer 'Grammatik des Deutschen im europäischen Kontext' ist also hinlänglich vorbereitet.
Elliptic flow analysis at RHIC with the Lee-Yang Zeroes method in a relativistic transport approach
(2006)
The Lee-Yang zeroes method is applied to study elliptic flow (v_2) in Au+Au collisions at sqrt s =200 A GeV, with the UrQMD model. In this transport approach, the true event plane is known and both the nonflow effects and event-by-event v_2 fluctuations exist. Although the low resolutions prohibit the application of the method for most central and peripheral collisions, the integral and differential elliptic flow from the Lee-Yang zeroes method agrees with the exact v_2 values very well for semi-central collisions.
The cumulant method is applied to study elliptic flow (v_2) in Au+Au collisions at sqrt s=200 AGeV, with the UrQMD model. In this approach, the true event plane is known and both the non-flow effects and event-by-event spatial (epsilon) and v_2 fluctuations exist. Qualitatively, the hierarchy of v_2 's from two, four and six-particle cumulants is consistent with the STAR data, however, the magnitude of v_2 in the UrQMD model is only 60% of the data. We find that the four and six-particle cumulants are good measures of the real elliptic flow over a wide range of centralities except for the most central and very peripheral events. There the cumulant method is affected by the v_2 fluctuations. In mid-central collisions, the four and six-particle cumulants are shown to give a good estimation of the true differential v_2, especially at large transverse momentum, where the two-particle cumulant method is heavily affected by the non-flow effects.
We propose to measure correlations of heavy-flavor hadrons to address the status of thermalization at the partonic stage of light quarks and gluons in high-energy nuclear collisions, shown on the example of azimuthal correlations of D-Dbar pairs. We show that hadronic interactions at the late stage can not disturb these correlations significantly. Thus, a decrease or the complete absence of these initial correlations indicates frequent interactions of heavy-flavor quarks in the partonic stage. Therefore, early thermalization of light quarks is likely to be reached. PACS numbers: 25.75.-q
A canonical partition function for the two-component excluded volume model is derived, leading to two di erent van der Waals approximations. The one is known as the Lorentz-Berthelot mixture and the other has been proposed recently. Both models are analysed in the canonical and grand canonical ensemble. In comparison with the one-component van der Waals excluded volume model the suppression of particle densities is reduced in these two-component formulations, but in two essentially di erent ways. Presently used multi-component models have no such reduction. They are shown to be not correct when used for components with di erent hard-core radii. For high temperatures the excluded volume interaction is refined by accounting for the Lorentz contraction of the spherical excluded volumes, which leads to a distinct enhancement of lighter particles. The resulting e ects on pion yield ratios are studied for AGS and SPS data.
One of the major problems in evolutionary biology is to elucidate the relationships between historical events and the tempo and mode of lineage divergence. The development of relaxed molecular clock models and the increasing availability of DNA sequences resulted in more accurate estimations of taxa divergence times. However, finding the link between competing historical events and divergence is still challenging. Here we investigate assigning constrained-age priors to nodes of interest in a time-calibrated phylogeny as a means of hypothesis comparison. These priors are equivalent to historic scenarios for lineage origin. The hypothesis that best explains the data can be selected by comparing the likelihood values of the competing hypotheses, modelled with different priors. A simulation approach was taken to evaluate the performance of the prior-based method and to compare it with an unconstrained approach. We explored the effect of DNA sequence length and the temporal placement and span of competing hypotheses (i.e. historic scenarios) on selection of the correct hypothesis and the strength of the inference. Competing hypotheses were compared applying a posterior simulation analogue of the Akaike Information Criterion and Bayes factors (obtained after calculation of the marginal likelihood with three estimators: Harmonic Mean, Stepping Stone and Path Sampling). We illustrate the potential application of the prior-based method on an empirical data set to compare competing geological hypotheses explaining the biogeographic patterns in Pleurodeles newts. The correct hypothesis was selected on average 89% times. The best performance was observed with DNA sequence length of 3500-10000 bp. The prior-based method is most reliable when the hypotheses compared are not temporally too close. The strongest inferences were obtained when using the Stepping Stone and Path Sampling estimators. The prior-based approach proved effective in discriminating between competing hypotheses when used on empirical data. The unconstrained analyses performed well but it probably requires additional computational effort. Researchers applying this approach should rely only on inferences with moderate to strong support. The prior-based approach could be applied on biogeographical and phylogeographical studies where robust methods for historical inferences are still lacking.
The properties of strange hadronic matter are studied in the context of the modified quark-meson coupling model using two substantially di erent sets of hyperon-hyperon (Y Y ) interactions. The first set is based on the Nijmegen hard core potential model D with slightly attractive Y Y interactions. The second potential set is based on the recent SU(3) extension of the Nijmegen soft-core potential NSC97 with strongly attractive Y Y interactions which may allow for deeply bound hypernuclear matter. The results show that, for the first potential set, the hyperon does not appear at all in the bulk at any baryon density and for all strangeness fractions. The binding energy curves of the resulting N system vary smoothly with density and the system is stable (or metastable if we include the weak force). However, the situation is drastically changed when using the second set where the hyperons appear in the system at large baryon densities above a critical strangeness fraction. We find strange hadronic matter undergoes a first order phase transition from a N system to a N for strangeness fractions fS > 1.2 and baryonic densities exceeding twice ordinary nuclear matter density. Furthermore, it is found that the system built of N is deeply bound. This phase transition a ects significantly the equation of state which becomes much softer and a substantial drop in energy density and pressure are detected as the phase transition takes place. PACS:21.65.+f, 24.85.+p, 12.39Ba
Hot hypernuclear matter is investigated in an explicit SU(3) quark model based on a mean field description of nonoverlapping baryon bags bound by the self-consistent exchange of scalar sigma, zeta and vector omega,phi mesons. The sigma, omega mean fields are assumed to couple to the u, d-quarks while the zeta ,phi mean fields are coupled to the s-quark. The coupling constants of the mean fields with the quarks are assumed to satisfy SU(6) symmetry. The calculations take into account the medium dependence of the bag parameter on the scalar fields sigma, zeta. We consider only the octet baryons N,Lambda,Sigma, Xi in hypernuclear matter. An ideal gas of the strange mesons K and K is introduced to keep zero net strangeness density. Our results for symmetric hypernuclear matter show that a phase transition takes place at a critical temperature around 180 MeV in which the scalar mean fields sigma, zeta take nonzero values at zero baryon density. Furthermore, the bag contants of the baryons decrease significantly at and above this critical temperature indicating the onset of quark deconfinement. The present results imply that the onset of quark deconfinement in SU(3) hypernuclear matter is much stronger than in SU(2) nuclear matter. PACS:21.65.+f, 24.85.+p, 12.39Ba
Irreversibility, steady state, and nonequilibrium physics in relativistic heavy ion collisions
(1999)
Heavy ion collisions at ultrarelativistic energies offer the opportunity to study the irreversibility of multiparticle processes. Together with the many-body decays of resonances, the multiparticle processes cause the system to evolve according to Prigogine s steady states rather than towards statistical equilibrium. These results are general and can be easily checked by any microscopic string-, transport-, or cascade model for heavy ion collisions. The absence of pure equilibrium states sheds light on the di culties of thermal models in describing the yields and spectra of hadrons, especially mesons, in heavy ion collisions at bombarding energies above 10 GeV/nucleon. PACS numbers: 25.75.-q, 05.70.Ln, 24.10.Lx
Homogeneous nucleation of quark gluon plasma, finite size effects and longlived metastable objects
(1998)
The general formalism of homogeneous nucleation theory is applied to study the hadronization pattern of the ultra-relativistic quark-gluon plasma (QGP) undergoing a first order phase transition. A coalescence model is proposed to describe the evolution dynamics of hadronic clusters produced in the nucle- ation process. The size distribution of the nucleated clusters is important for the description of the plasma conversion. The model is most sensitive to the initial conditions of the QGP thermalization, time evolution of the energy den- sity, and the interfacial energy of the plasma hadronic matter interface. The rapidly expanding QGP is first supercooled by about T = T Tc = 4 6%. Then it reheats again up to the critical temperature Tc. Finally it breaks up into hadronic clusters and small droplets of plasma. This fast dynamics occurs within the first 5 10 fm/c. The finite size e ects and fluctuations near the critical temperature are studied. It is shown that a drop of longitudinally expanding QGP of the transverse radius below 4.5 fm can display a long-lived metastability. However, both in the rapid and in the delayed hadronization scenario, the bulk pion yield is emitted by sources as large as 3 4.5 fm. This may be detected experimentally both by a HBT interferometry signal and by the analysis of the rapidity distributions of particles in narrow pT -intervals at small |pT | on an event-by-event basis. PACS numbers: 12.38.Mh, 24.10.Pa, 25.75.-q, 64.60.Qb
We reexamine the scenario of homogeneous nucleation of the quark-gluon plasma produced in ultra-relativistic heavy ion collisions. A generalization of the standard nucleation theory to rapidly expanding system is proposed. The nucleation rate is derived via the new scaling parameter Z. It is shown that the size distribution of hadronic clusters plays an important role in the dynamics of the phase transition. The longitudinally expanding system is supercooled to about 3 6%, then it is reheated, and the hadronization is completed within 6 10 fm/c, i.e. 5 10 times faster than it was estimated earlier, in a strongly nonequilibrium way. PACS: 12.38.Mh; 12.39.Ba; 25.75.-q; 64.60.Qb
Release of neuropeptides from dense core vesicles (DCVs) is essential for neuromodulation. Compared to the release of small neurotransmitters, much less is known about the mechanisms and proteins contributing to neuropeptide release. By optogenetics, behavioral analysis, electrophysiology, electron microscopy, and live imaging, we show that synapsin SNN-1 is required for cAMP-dependent neuropeptide release in Caenorhabditis elegans hermaphrodite cholinergic motor neurons. In synapsin mutants, behaviors induced by the photoactivated adenylyl cyclase bPAC, which we previously showed to depend on acetylcholine and neuropeptides (Steuer Costa et al., 2017), are altered like in animals with reduced cAMP. Synapsin mutants have slight alterations in synaptic vesicle (SV) distribution, however, a defect in SV mobilization was apparent after channelrhodopsin-based photostimulation. DCVs were largely affected in snn-1 mutants: DCVs were ∼30% reduced in synaptic terminals, and not released following bPAC stimulation. Imaging axonal DCV trafficking, also in genome-engineered mutants in the serine-9 protein kinase A phosphorylation site, showed that synapsin captures DCVs at synapses, making them available for release. SNN-1 co-localized with immobile, captured DCVs. In synapsin deletion mutants, DCVs were more mobile and less likely to be caught at release sites, and in non-phosphorylatable SNN-1B(S9A) mutants, DCVs traffic less and accumulate, likely by enhanced SNN-1 dependent tethering. Our work establishes synapsin as a key mediator of neuropeptide release.
Snapshots of acetyl-CoA synthesis, the final step of CO₂ fixation in the Wood-Ljungdahl pathway
(2024)
In the ancient microbial Wood-Ljungdahl pathway, CO2 is fixed in a multi-step process with acetyl-CoA synthesis at the bifunctional carbon monoxide dehydrogenase/acetyl-CoA synthase complex (CODH/ACS). Here, we present catalytic snapshots of the CODH/ACS from the gas-converting acetogen Clostridium autoethanogenum, characterizing the molecular choreography of the overall reaction including electron transfer to the CODH for CO2 reduction, methyl transfer from the corrinoid iron-sulfur protein (CoFeSP) partner to the ACS active site and acetyl-CoA production. Unlike CODH, the multidomain ACS undergoes large conformational changes to form an internal connection to the CODH active site, accommodate the CoFeSP for methyl transfer and protect the reaction intermediates. Altogether, the structures allow us to draw a detailed reaction mechanism of this enzyme crucial for CO2 fixation in anaerobic organisms.
Different modification pathways for m1A58 incorporation in yeast elongator and initiator tRNAs
(2022)
As essential components of the cellular protein synthesis machineries, tRNAs undergo a tightly controlled biogenesis process, which include the incorporation of a large number of posttranscriptional chemical modifications. Maturation defaults resulting in lack of modifications in the tRNA core may lead to the degradation of hypomodified tRNAs by the rapid tRNA decay (RTD) and nuclear surveillance pathways. Although modifications are typically introduced in tRNAs independently of each other, several modification circuits have been identified in which one or more modifications stimulate or repress the incorporation of others. We previously identified m1A58 as a late modification introduced after more initial modifications, such as Ѱ55 and T54 in yeast elongator tRNAPhe. However, previous reports suggested that m1A58 is introduced early along the tRNA modification process, with m1A58 being introduced on initial transcripts of initiator tRNAiMet, and hence preventing its degradation by the nuclear surveillance and RTD pathways. Here, aiming to reconcile this apparent inconsistency on the temporality of m1A58 incorporation, we examined the m1A58 modification pathways in yeast elongator and initiator tRNAs. For that, we first implemented a generic approach enabling the preparation of tRNAs containing specific modifications. We then used these specifically modified tRNAs to demonstrate that the incorporation of T54 in tRNAPhe is directly stimulated by Ѱ55, and that the incorporation of m1A58 is directly and individually stimulated by Ѱ55 and T54, thereby reporting on the molecular aspects controlling the Ѱ55 → T54 → m1A58 modification circuit in yeast elongator tRNAs. We also show that m1A58 is efficiently introduced on unmodified tRNAiMet, and does not depend on prior modifications. Finally, we show that the m1A58 single modification has tremendous effects on the structural properties of yeast tRNAiMet, with the tRNA elbow structure being properly assembled only when this modification is present. This rationalizes on structural grounds the degradation of hypomodified tRNAiMet lacking m1A58 by the nuclear surveillance and RTD pathways.
Layered {\alpha}-RuCl3 is a promising material to potentially realize the long-sought Kitaev quantum spin liquid with fractionalized excitations. While evidence of this exotic state has been reported under a modest in-plane magnetic field, such behavior is largely inconsistent with theoretical expectations of Kitaev phases emerging only in out-of-plane fields. These predicted field-induced states have been mostly out of reach due to the strong easy-plane anisotropy of bulk crystals, however. We use a combination of tunneling spectroscopy, magnetotransport, electron diffraction, and ab initio calculations to study the layer-dependent magnons, anisotropy, structure, and exchange coupling in atomically thin samples. Due to structural distortions, the sign of the average off-diagonal exchange changes in monolayer {\alpha}-RuCl3, leading to a reversal of magnetic anisotropy to easy-axis. Our work provides a new avenue to tune the magnetic interactions in {\alpha}-RuCl3 and allows theoretically predicted quantum spin liquid phases for out-of-plane fields to be more experimentally accessible.
Ribosomes catalyze protein synthesis by cycling through various functional states. These states have been extensively characterized in vitro, yet their distribution in actively translating human cells remains elusive. Here, we optimized a cryo-electron tomography-based approach and resolved ribosome structures inside human cells with a local resolution of up to 2.5 angstroms. These structures revealed the distribution of functional states of the elongation cycle, a Z tRNA binding site and the dynamics of ribosome expansion segments. In addition, we visualized structures of Homoharringtonine, a drug for chronic myeloid leukemia treatment, within the active site of the ribosome and found that its binding reshaped the landscape of translation. Overall, our work demonstrates that structural dynamics and drug effects can be assessed at near-atomic detail within human cells.
The maximum recoverable strain of most crystalline solids is less than 1% because plastic deformation or fracture usually occurs at a small strain. In this work, we show that a SrNi2P2 micropillar exhibits pseudoelasticity with a large maximum recoverable strain of ~14% under uniaxial compression via unique reversible structural transformation, double lattice collapse-expansion that is repeatable under cyclic loading. Its high yield strength (~3.8±0.5 GPa) and large maximum recoverable strain bring out the ultrahigh modulus of resilience (~146±19MJ/m3) a few orders of magnitude higher than that of most engineering materials. The double lattice collapse-expansion mechanism shows stress-strain behaviors similar with that of conventional shape memory alloys, such as hysteresis and thermo-mechanical actuation, even though the structural changes involved are completely different. Our work suggests that the discovery of a new class of high performance ThCr2Si2-structured materials will open new research opportunities in the field of pseudoelasticity
Liebesbriefe von Kindern, Jugendlichen und Erwachsenen : eine Textsorte im lebenszeitlichen Wandel
(2003)
Das Alter als soziolinguistische und – mit Bezug auf die Historizität des sozialen Alltags – als sozialhistorische Grösse ist in seiner Wirkung auf die Gestaltung des Liebesbriefs wenig offensichtlich. Unbestritten dürfte aber wohl sein, dass nicht alterslose Menschen einander Liebesbriefe schreiben. Und – Alter prägt, wie dies die hier vorliegende empirische Analyse zeigen wird, die Textsorte Liebesbrief vielleicht stärker als gemeinhin angenommen. Bereits die Briefstellerliteratur der Jahrhundertwende zeigt deutlich eine Altersspezifik der Sprache des Liebesbriefs. ...
Der Liebesbrief des 20. Jahrhunderts ist Ausdruck einer konkreten lebensweltlichen und historisch zu verortenden Praxis der Liebeskommunikation. Liebesbriefe sind Brautbriefe, Liebesbekenntnisse, Berichte aus dem Alltag, Soldatenbriefe, Vereinbarungen von Treffen, E-Mail-Korrespondenzen, Flirtbriefe und Zettelchen – es gibt eine reiche Palette an Funktionen und Typen. Im Hinblick auf eine Geschichte des Liebesbriefs im 20. Jahrhunderts zeigte sich, dass im Liebesbrief neben der Liebeserklärung auch „Beziehungsarbeit“ und besonders aber die Konstruktion von Intimität eine zentrale Rolle spielt. Die Kritik an der Sprache der Liebe und des Liebesbriefs (des 19. Jahrhunderts) kann bereits in den 1920er Jahren beobachtet werden. Zu einem Codewechsel kommt es in Briefen der 1960er Jahre. Die Schriftlichkeit des Liebesbriefs entfernt sich allmählich von einer ausschließlichen Schreibschriftlichkeit. Der Liebesbrief wird mehr und mehr zu einem Sprache-Bild-Text. Die neuen Medien der Liebesschriftlichkeit zeigen eine Mediatisierung auch im Bereich des Liebesdiskurses: neben neuen Liebesbrieftypen, wie dem Flirtbrief, bilden sich neue Liebesbeziehungstypen heraus. Darüber hinaus fungieren die neuen Medien immer schon selbstreflexiv als Metakommunikatoren der Modernität.
Klugheit wird gemeinhin als das Gegenteil von Torheit aufgefasst. Auf diese Weise erfährt sie eine sprachlich vorstrukturierte positive Bewertung und erhält einen ausgezeichneten gesellschaftlichen Status. "Positiv" bedeutet eine Verknüpfung mit spezifischen je gesellschaftlich richtigen Wertmassstäben, die aber in unterschiedlichen Milieus und Regionen durchaus verschieden ausfallen. Diese bilden den impliziten Subtext für die alltägliche Zuschreibung von "Klugheit". Klugheit fokussiert das Verhalten der Menschen, die Handlungen, die Performanz. Klugheit wird denjenigen Personen zugeschrieben, die "das Richtige" tun, und nachdem sie das Richtige getan haben, etabliert sich erst das Kriterium für die Richtigkeit dieser Beurteilung: der Ausgang der Geschichte. Klugheit wird zwar im vornhinein behauptet, stellt sich aber erst im Nachhinein heraus: denn sie misst sich nicht an der vorgeführten Handlung selbst, sondern am Ausgang der "Geschichte". Eine Bauerntochter handelt dann klug, wenn ihre Handlungen zu einem – im Sinne des Erzählers – guten Ende führen, zu einem Happy-End sozusagen. ...
Während der Brief in Zeiten von persönlichen Krisen und Konflikten mancherlei Unannehmlichkeiten aus dem Kommunikationsweg räumt, stellt der Kontext Krieg für das Briefeschreiben in vielerlei Hinsicht eine Herausforderung dar. Der Privatbrief (Epistula familiaris) ist in der ersten Hälfte des 20. Jahrhunderts in Westeuropa – das heisst auch zur Zeit des 2. Weltkriegs – das wichtigste Medium informeller Distanzkommunikation, welche im Allgemeinen durch Inoffizialität und Spontaneität, durch Individualität und Vertraulichkeit gekennzeichnet ist. In der Regel ist der Privatbrief im juristischen Sinne nicht verfügbar. Ein Kennzeichen ist somit auch seine Nichtreproduzierbarkeit. Neben der thematischen Offenheit macht sich meist eine stärkere stilistische Freiheit bemerkbar. Zeichen von Flüchtigkeit oder Sorgfalt sind ausser den Formalia des Datums, der Anrede, des Textkörpers und der Unterschrift, über das geschriebene Wort hinaus nonverbale Informationen wie die Lesbarkeit der Schrift, die Wahl des Papiers, Schreibwerkzeug sowie die Länge eines Briefes (vgl. Ermert 1979, Nickisch 1991, Beyer/ Täubrich 1996, Zott 2003). Der Privatbrief wird zwar im graphischen Medium der Schrift realisiert, steht aber stilistisch der konzeptionellen "Mündlichkeit" näher. (Koch/ Oesterreicher 1994, 587) Der private Briefwechsel wird spontan aufgenommen und kann in der Regel ohne Zwang abgebrochen werden (vgl. Zott 2003). ...
Die Ressource "Wissen" rückte in den letzten Jahrzehnten als Quelle wissenschaftlicher Innovation immer stärker ins Zentrum des Interesses. Diese Fokussierung mündete in eine Selbstreflexion der Wissenschaft und der wissenschaftlichen Disziplinen: Thematisiert werden vor allem die Art und Weise, wie Wissen gewonnen wird, sowie die damit zusammenhängende Frage nach der Konstruktion von Wissenschaftlichkeit, womit das Bewusstsein gleichzeitig auf die mehr und mehr sich auflösende Abgrenzung zwischen den Disziplinen beziehungsweise zwischen den drei hauptsächlichen Wissenschaftskulturen, von Natur-, Geistes- und Kultur- sowie Sozialwissenschaften gelenkt wird. Innerhalb und außerhalb der Universitäten bildeten und bilden sich nicht immer klar verortbare "trading zones" (Gallison 1997), in denen neue Formen und Techniken der Wissensproduktion und Wissensvermittlung geprüft, geübt und teilweise auch institutionalisiert werden. ...
We compute bremsstrahlung arising from the acceleration of individual charged baryons and mesons during the time evolution of high-energy Au+Au collisions at the Relativistic Heavy Ion Collider using a microscopic transport model. We elucidate the connection between bremsstrahlung and charge stop- ping by colliding artificial pure proton on pure neutron nuclei. From the inten- sity of low energy bremsstrahlung, the time scale and the degree of stopping could be accurately extracted without measuring any hadronic observables. PACS: 25.75.-q, 13.85.Qk
The snake pipefish, Entelurus aequoreus (Linnaeus, 1758), is a slender, up to 60 cm long, northern Atlantic fish that dwells in open seagrass habitats and has recently expanded its distribution range. The snake pipefish is part of the family Syngnathidae (seahorses and pipefish) that has undergone several characteristic morphological changes, such as loss of pelvic fins and elongated snout. Here, we present a highly contiguous, near chromosome-scale genome of the snake pipefish assembled as part of a university master’s course. The final assembly has a length of 1.6 Gbp in 7,391 scaffolds, a scaffold and contig N50 of 62.3 Mbp and 45.0 Mbp and L50 of 12 and 14, respectively. The largest 28 scaffolds (>21 Mbp) span 89.7% of the assembly length. A BUSCO completeness score of 94.1% and a mapping rate above 98% suggest a high assembly completeness. Repetitive elements cover 74.93% of the genome, one of the highest proportions so far identified in vertebrate genomes. Demographic modeling using the PSMC framework indicates a peak in effective population size (50 – 100 kya) during the last interglacial period and suggests that the species might largely benefit from warmer water conditions, as seen today. Our updated snake pipefish assembly forms an important foundation for further analysis of the morphological and molecular changes unique to the family Syngnathidae.
All giraffe (Giraffa) were previously assigned to a single species (G. Camelopardalis) and nine subspecies. However, multi-locus analyses of all subspecies have shown that there are four genetically distinct clades and suggest four giraffe species. This conclusion might not be fully accepted due to limited data and lack of explicit gene flow analyses. Here we present an extended study based on 21 independent nuclear loci from 137 individuals. Explicit gene flow analyses identify less than one migrant per generation, including between the closely related northern and reticulated giraffe. Thus, gene flow analyses and population genetics of the extended dataset confirm four genetically distinct giraffe clades and support four independent giraffe species. The new findings call for a revision of the IUCN classification of giraffe taxonomy. Three of the four species are threatened with extinction, mostly occurring in politically unstable regions, and as such, require the highest conservation support possible.
Background: Biological psychiatry aims to understand mental disorders in terms of altered neurobiological pathways. However, for one of the most prevalent and disabling mental disorders, Major Depressive Disorder (MDD), patients only marginally differ from healthy individuals on the group-level. Whether Precision Psychiatry can solve this discrepancy and provide specific, reliable biomarkers remains unclear as current Machine Learning (ML) studies suffer from shortcomings pertaining to methods and data, which lead to substantial over-as well as underestimation of true model accuracy.
Methods: Addressing these issues, we quantify classification accuracy on a single-subject level in N=1,801 patients with MDD and healthy controls employing an extensive multivariate approach across a comprehensive range of neuroimaging modalities in a well-curated cohort, including structural and functional Magnetic Resonance Imaging, Diffusion Tensor Imaging as well as a polygenic risk score for depression.
Findings Training and testing a total of 2.4 million ML models, we find accuracies for diagnostic classification between 48.1% and 62.0%. Multimodal data integration of all neuroimaging modalities does not improve model performance. Similarly, training ML models on individuals stratified based on age, sex, or remission status does not lead to better classification. Even under simulated conditions of perfect reliability, performance does not substantially improve. Importantly, model error analysis identifies symptom severity as one potential target for MDD subgroup identification.
Interpretation: Although multivariate neuroimaging markers increase predictive power compared to univariate analyses, single-subject classification – even under conditions of extensive, best-practice Machine Learning optimization in a large, harmonized sample of patients diagnosed using state-of-the-art clinical assessments – does not reach clinically relevant performance. Based on this evidence, we sketch a course of action for Precision Psychiatry and future MDD biomarker research.
Background Vasoplegic syndrome is frequently observed during cardiac surgery and resembles a complication of high mortality and morbidity. There is a clinical need for therapy and prevention of vasoplegic syndrome during complex cardiac surgical procedures. Therefore, we investigated different strategies in a porcine model of vasoplegia.
Methods We evaluated new medical therapies and prophylaxis to avoid vasoplegic syndrome in a porcine model. After induction of anesthesia, cardiopulmonary bypass was established through median sternotomy and central cannulation. Prolonged aortic cross-clamping (120 min) simulated a complex surgical procedure. The influence of sevoflurane-guided anesthesia (sevoflurane group) and the administration of glibenclamide (glibenclamide group) were compared to a control group, which received standard anesthesia using propofol. Online hemodynamic assessment was performed using PiCCO® measurements. In addition, blood and tissue samples were taken to evaluate hemodynamic effects and the degree of inflammatory response.
Results Glibenclamide was able to break through early vasoplegic syndrome by raising the blood pressure and systemic vascular resistance as well as less need of norepinephrine doses. Sevoflurane reduced the occurrence of the vasoplegic syndrome in the mean of stable blood pressure and less need of norepinephrine doses.
Conclusion Glibenclamide could serve as a potent drug to reduce effects of vasoplegic syndrome. Sevoflurane anesthesia during cardiopulmonary bypass shows less occurrence of vasoplegic syndrome and therefore could be used to prevent it in high-risk patients.
Clinical Perspective; what is new?
* to our knowledge, this is the first randomized in vivo study evaluating the hemodynamic effects of glibenclamide after the onset of vasoplegic syndrome
* furthermore according to literature research, there is no study showing the effect of sevoflurane-guided anesthesia on the occurrence of a vasoplegic syndrome
Clinical Perspective; clinical implications?
to achieve better outcomes after complex cardiac surgery there is a need for optimized drug therapy and prevention of the vasoplegic syndrome
In this work, the phase diagram of the 2+1-dimensional Gross-Neveu model is investigated with baryon chemical potential as well as chiral chemical potential in the mean-field approximation. We study the theory using two lattice discretizations, which are both based on naive fermions. An inhomogeneous chiral phase is observed only for one of the two discretizations. Our results suggest that this phase disappears in the continuum limit.
In this work, inhomogeneous chiral phases are studied in a variety of Four-Fermion and Yukawa models in 2+1 dimensions at zero and non-zero temperature and chemical potentials. Employing the mean-field approximation, we do not find indications for an inhomogeneous phase in any of the studied models. We show that the homogeneous phases are stable against inhomogeneous perturbations. At zero temperature, full analytic results are presented.
Transfer RNA fragments replace microRNA regulators of the cholinergic post-stroke immune blockade
(2020)
Stroke is a leading cause of death and disability. Recovery depends on balance between inflammatory response and immune suppression, which can be CNS-protective but may worsen prognosis by increasing patients’ susceptibility to infections. Peripheral cholinergic blockade of immune reactions fine-tunes this immune response, but its molecular regulators are unknown. Therefore, we sought small RNA balancers of the cholinergic anti-inflammatory pathway in peripheral blood from ischemic stroke patients. Using RNA-sequencing and RT-qPCR, we discovered in patients’ blood on day 2 after stroke a “change of guards” reflected in massive decreases in microRNAs (miRs) and increases in transfer RNA fragments (tRFs) targeting cholinergic transcripts. Electrophoresis-based size-selection followed by RT-qPCR validated the top 6 upregulated tRFs in a separate cohort of stroke patients, and independent small RNA-sequencing datasets presented post-stroke enriched tRFs as originating from lymphocytes and monocytes. In these immune compartments, we found CD14+ monocytes to express the highest amounts of cholinergic transcripts. In-depth analysis of CD14+ regulatory circuits revealed minimally overlapping subsets of transcription factors carrying complementary motifs to miRs or tRFs, indicating different roles for the stroke-perturbed members of these small RNA species. Furthermore, LPS-stimulated murine RAW264.7 cells presented dexamethasone-suppressible upregulation of the top 6 tRFs identified in human patients, indicating an evolutionarily conserved and pharmaceutically treatable tRF response to inflammatory cues. Our findings identify tRF/miR subgroups which may co-modulate the homeostatic response to stroke in patients’ blood and open novel venues for establishing RNA-targeted concepts for post-stroke diagnosis and therapeutics.
The behavior of hadronic matter at high baryon densities is studied within Ultrarelativistic Quantum Molecular Dynamics (URQMD). Baryonic stopping is observed for Au+Au collisions from SIS up to SPS energies. The excitation function of flow shows strong sensitivities to the underlying equation of state (EOS), allowing for systematic studies of the EOS. Effects of a density dependent pole of the rho-meson propagator on dilepton spectra are studied for different systems and centralities at CERN energies.
A key competence for open-ended learning is the formation of increasingly abstract representations useful for driving complex behavior. Abstract representations ignore specific details and facilitate generalization. Here we consider the learning of abstract representations in a multi-modal setting with two or more input modalities. We treat the problem as a lossy compression problem and show that generic lossy compression of multimodal sensory input naturally extracts abstract representations that tend to strip away modalitiy specific details and preferentially retain information that is shared across the different modalities. Furthermore, we propose an architecture to learn abstract representations by identifying and retaining only the information that is shared across multiple modalities while discarding any modality specific information.
Pseudomonas aeruginosa is a human pathogen that causes health-care associated blood stream infections (BSI). Although P. aeruginosa BSI are associated with high mortality rates, the clinical relevance of pathogen-derived prognostic biomarker to identify patients at risk for unfavorable outcome remains largely unexplored. We found novel pathogen-derived prognostic biomarker candidates by applying a multi-omics approach on a multicenter sepsis patient cohort. Multi-level Cox regression was used to investigate the relation between patient characteristics and pathogen features (2298 accessory genes, 1078 core protein levels, 107 parsimony-informative variations in reported virulence factors) with 30-day mortality. Our analysis revealed that presence of the helP gene encoding a putative DEAD-box helicase was independently associated with a fatal outcome (hazard ratio 2.01, p = 0.05). helP is located within a region related to the pathogenicity island PAPI-1 in close proximity to a pil gene cluster, which has been associated with horizontal gene transfer. Besides helP, elevated protein levels of the bacterial flagellum protein FliL (hazard ratio 3.44, p < 0.001) and of a bacterioferritin-like protein (hazard ratio 1.74, p = 0.003) increased the risk of death, while high protein levels of a putative aminotransferase were associated with an improved outcome (hazard ratio 0.12, p < 0.001). The prognostic potential of biomarker candidates and clinical factors was confirmed with different machine learning approaches using training and hold-out datasets. The helP genotype appeared the most attractive biomarker for clinical risk stratification due to its relevant predictive power and ease of detection.
Reduced neutralization of SARS-CoV-2 Omicron variant by vaccine sera and monoclonal antibodies
(2021)
Due to numerous mutations in the spike protein, the SARS-CoV-2 variant of concern Omicron (B.1.1.529) raises serious concerns since it may significantly limit the antibody-mediated neutralization and increase the risk of reinfections. While a rapid increase in the number of cases is being reported worldwide, until now there has been uncertainty about the efficacy of vaccinations and monoclonal antibodies. Our in vitro findings using authentic SARS-CoV-2 variants indicate that in contrast to the currently circulating Delta variant, the neutralization efficacy of vaccine-elicited sera against Omicron was severely reduced highlighting T-cell mediated immunity as essential barrier to prevent severe COVID-19. Since SARS-CoV-2 Omicron was resistant to casirivimab and imdevimab, genotyping of SARS-CoV-2 may be needed before initiating mAb treatment. Variant-specific vaccines and mAb agents may be required to treat COVID-19 due to Omicron and other emerging variants of concern.
Reduced neutralization of SARS-CoV-2 Omicron variant by vaccine sera and monoclonal antibodies
(2021)
Due to numerous mutations in the spike protein, the SARS-CoV-2 variant of concern Omicron (B.1.1.529) raises serious concerns since it may significantly limit the antibody-mediated neutralization and increase the risk of reinfections. While a rapid increase in the number of cases is being reported worldwide, until now there has been uncertainty about the efficacy of vaccinations and monoclonal antibodies. Our in vitro findings using authentic SARS-CoV-2 variants indicate that in contrast to the currently circulating Delta variant, the neutralization efficacy of vaccine-elicited sera against Omicron was severely reduced highlighting T-cell mediated immunity as essential barrier to prevent severe COVID-19. Since SARS-CoV-2 Omicron was resistant to casirivimab and imdevimab, genotyping of SARS-CoV-2 may be needed before initiating mAb treatment. Variant-specific vaccines and mAb agents may be required to treat COVID-19 due to Omicron and other emerging variants of concern.