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Soil frequently occurs as a contaminant on numerous sea, land and air transport pathways. It can carry unwanted invasive species, is widely recognized as a biosecurity risk, and is usually strictly regulated by biosecurity authorities. However, little is known about relative risk levels between pathways, thus authorities have limited capability to identify and target the riskiest soil pathways for management. We conducted a an experiment to test the hypotheses that biosecurity risks from soil organisms will increase both with declining transport duration and with increasing protection from environmental extremes. Soil was collected from two sites, a native forest remnant and an orchard, and stored on, in and under sea containers, or in cupboards, and assayed after 0, 3, 6 and 12 months for bacteria, fungi, nematodes and seeds. Results showed that viability of Pseudomonas spp., bacteria, nematodes and plants declined over 12 months, irrespective of soil source. Also, mortality of most biota was higher when exposed to sunlight, moisture and desiccation than when protected. However, bacterial and fungal numbers were higher in exposed environments, possibly due to ongoing colonization of exposed soil by airborne propagules. The results were consistent with our observations of organisms in soil intercepted from airports and sea ports, and indicated there is potential to rank risks from transported soils based partly on transport duration and environmental exposure. This would help authorities to optimally allocate management resources according to pathway-specific risks.
A compreensão histórica de literatura no primeiro romantismo alemão decorre, entre outros aspectos, dos escritos de Winckelmann, Herder, Lessing, assim como da relação dialética entre o clássico e o romântico estabelecida por Goethe e Schiller. Nesse contexto, a aproximação antitética entre o antigo e o romântico tem um papel central na determinação das principais teorias românticas de literatura. O artigo investiga e discute a importância da antinomia entre o antigo e o moderno na constituição e desenvolvimento das teorias literárias de Friedrich Schlegel no primeiro romantismo alemão
Ebenso wie Pierre Bourdieu ist sein Schüler Loïc Wacquant einer der selten gewordenen Intellektuellen, die ihre Profession, die Soziologie, nutzen um soziale Ungleichheiten und gesellschaftliche Machtverhältnisse nicht nur zu analysieren, sondern auch versuchen, damit auf öffentliche Debatten zu einzuwirken. Daher ist Wacquants Aufsatz nicht nur als eine interessante Diskussion der Frage, ob Bourdieus Werk in der Stadtsoziologie angemessen und korrekt verwendet werde, zu verstehen. Der in dem Text formulierte Appell, Bourdieus Arbeit auch innerhalb der Stadtsoziologie stärker anzuwenden, ist – vor dem Hintergrund zunehmender sozialer Ungleichheit, Armut, Marginalisierung und einem Aufschwung des Rechtspopulismus – eine Forderung nach einer soziologischen Betrachtung dieser Probleme in der Stadt. Was kann nun also das Werk von Pierre Bourdieu zur Analyse der heutigen sozialen Probleme in der Stadt in Deutschland beitragen?
Ilse Aichingers 'Spiegelgeschichte' ist einer der schon früh zum Klassiker avancierten Prosatexte der österreichischen Nachkriegsliteratur. Nachdem Aichinger seit Anfang des Jahres 1948 gut eineinhalb Jahre daran gearbeitet hatte, erschien der Text erstmals in drei Fortsetzungen im August 1949 in der Wiener Tageszeitung, 1952 folgte die Publikation in der Rede unter dem Galgen betitelten Ausgabe von Hans Weigel. Isoliert man das erzählte Geschehen aus der von einer anonymen Du-Stimme anachronisch erzählten Analepse, erzählt man die spiegelverkehrt vergebenen Informationen im Text also 'realistisch' nach, so hat man es zunächst mit einem recht simplen plot zu tun: Eine junge Frau erlebt mit dem frühen Verlust ihrer Mutter und der damit einhergehenden Verantwortung für ihre jüngeren Brüder eine schwere Kindheit, verliebt sich, träumt von einer guten Zukunft, wird ungewollt schwanger und lässt von einer Kurpfuscherin eine Abtreibung vornehmen, an deren Folgen sie stirbt. Aichinger verweigert ihrer Geschichte aber einen solchermaßen linearen Verlauf und unterminiert alle traditionellen "Parameter der realistisch-mimetischen Schreib- und Leseweise", indem sie logische wie chronologische Denkmuster aushebelt. Ihre Geschichte beginnt mit einem Irrealis, nämlich der Imagination des Tot-Seins: Die im Krankenhaus im Sterben liegende Protagonistin antizipiert die eigene Begräbnissituation, schickt sich selbst aus ihrem Grab wieder hinaus und vergegenwärtigt sich ihr Leben stationsweise raffend jeweils gegenchronologisch vom Grab, über die Leichenhalle, das Sterbebett, die Abtreibung, die Liebesgeschichte, den ersten Geschlechtsverkehr, die Schulzeit und die Kindheit bis zu ihrer Geburt.
Este escrito reconstruye la problematización que Wellmer hace del planteamiento adorniano sobre el hundimiento de la metafísica y su articulación con una eventual alternativa estética para, inmediatamente, explorar la propuesta integral de la tercera Escuela de Frankfurt en torno a la eticidad democrática, sustentable desde los planteamientos de Dubiel, Honneth y Wellmer, como alternativa para una superación de las aporías que Adorno y la primera Escuela de Frankfurt no logran superar, categoría que nos remite en Habermas a su noción del patriotismo constitucional donde adquiere toda su proyección política postconvencional la eticidad democrática y que concurre en la figura de una opinión pública contestataria, que tanto Habermas como Nancy Fraser han querido fundamentar, como opción contra hegemónica y potencialmente emancipatoria en las sociedades en conflicto, para finalmente articular lo anterior con el talante cosmopolita del republicanismo que enfatiza, desde Nussbaum, Bohman y de nuevo Fraser, la perspectiva transnacional que las aspiraciones emancipatorias pueden tener en un mundo global, cuyas sinergias confluyen y concretan no solo estrategias de convivencia democrática nacionales e internacionales sino además, a través de las mismas, la superación concreta —no meramente ética o estética— de fenómenos de autoritarismo político y alienación social, en tanto mediación idónea, aunque parcial, de reconciliación en las sociedades complejas.
NEK family kinases are serine/threonine kinases that have been functionally implicated in the regulation of the disjunction of the centrosome, the assembly of the mitotic spindle, the function of the primary cilium and the DNA damage response. NEK1 shows pleiotropic functions and has been found to be mutated in cancer cells, ciliopathies such as the polycystic kidney disease, as well as in the genetic diseases short-rib thoracic dysplasia, Mohr-syndrome and amyotrophic lateral sclerosis. NEK1 is essential for the ionizing radiation DNA damage response and priming of the ATR kinase and of Rad54 through phosphorylation. Here we report on the structure of the kinase domain of human NEK1 in its apo- and ATP-mimetic inhibitor bound forms. The inhibitor bound structure may allow the design of NEK specific chemo-sensitizing agents to act in conjunction with chemo- or radiation therapy of cancer cells. Furthermore, we characterized the dynamic protein interactome of NEK1 after DNA damage challenge with cisplatin. Our data suggest that NEK1 and its interaction partners trigger the DNA damage pathways responsible for correcting DNA crosslinks.
AirCore-HR : a high-resolution column sampling to enhance the
vertical description of CH₄ and CO₂
(2017)
An original and innovative sampling system called AirCore was presented by NOAA in 2010 (Karion et al., 2010). It consists of a long ( > 100 m) and narrow (< 1 cm) stainless steel tube that can retain a profile of atmospheric air. The captured air sample has then to be analyzed with a gas analyzer for trace mole fraction. In this study, we introduce a new AirCore aiming to improve resolution along the vertical with the objectives to (i) better capture the vertical distribution of CO2 and CH4, (ii) provide a tool to compare AirCores and validate the estimated vertical resolution achieved by AirCores. This (high-resolution) AirCore-HR consists of a 300 m tube, combining 200 m of 0.125 in. (3.175 mm) tube and a 100 m of 0.25 in. (6.35 mm) tube. This new configuration allows us to achieve a vertical resolution of 300 m up to 15 km and better than 500 m up to 22 km (if analysis of the retained sample is performed within 3 h). The AirCore-HR was flown for the first time during the annual StratoScience campaign from CNES in August 2014 from Timmins (Ontario, Canada). High-resolution vertical profiles of CO2 and CH4 up to 25 km were successfully retrieved. These profiles revealed well-defined transport structures in the troposphere (also seen in CAMS-ECMWF high-resolution forecasts of CO2 and CH4 profiles) and captured the decrease of CO2 and CH4 in the stratosphere. The multi-instrument gondola also carried two other low-resolution AirCore-GUF that allowed us to perform direct comparisons and study the underlying processing method used to convert the sample of air to greenhouse gases vertical profiles. In particular, degrading the AirCore-HR derived profiles to the low resolution of AirCore-GUF yields an excellent match between both sets of CH4 profiles and shows a good consistency in terms of vertical structures. This fully validates the theoretical vertical resolution achievable by AirCores. Concerning CO2 although a good agreement is found in terms of vertical structure, the comparison between the various AirCores yields a large and variable bias (up to almost 3 ppm in some parts of the pro- files). The reasons of this bias, possibly related to the drying agent used to dry the air, are still being investigated. Finally, the uncertainties associated with the measurements are assessed, yielding an average uncertainty below 3 ppb for CH4 and 0.25 ppm for CO2 with the major source of uncertainty coming from the potential loss of air sample on the ground and the choice of the starting and ending point of the collected air sample inside the tube. In an ideal case where the sample would be fully retained, it would be possible to know precisely the pressure at which air was sampled last and thus to improve the overall uncertainty to about 0.1 ppm for CO2 and 2 ppb for CH4
New neutron cross section measurements of minor actinides have been performed recently in order to reduce the uncertainties in the evaluated data, which is important for the design of advanced nuclear reactors and, in particular, for determining their performance in the transmutation of nuclear waste. We have measured the 241Am(n,γ) cross section at the n_TOF facility between 0.2 eV and 10 keV with a BaF2 Total Absorption Calorimeter, and the analysis of the measurement has been recently concluded. Our results are in reasonable agreement below 20 eV with the ones published by C. Lampoudis et al. in 2013, who reported a 22% larger capture cross section up to 110 eV compared to experimental and evaluated data published before. Our results also indicate that the 241Am(n,γ) cross section is underestimated in the present evaluated libraries between 20 eV and 2 keV by 25%, on average, and up to 35% for certain evaluations and energy ranges.
Pediatric patients with recurrent, refractory or advanced soft tissue sarcoma (STS) who are simultaneously showing signs of cumulative treatment toxicity are in need of novel therapies. In this preclinical analysis, we identified ErbB2 as a targetable antigen on STS cells and used cytokine-induced killer (CIK) cells transduced with the lentiviral 2nd-generation chimeric antigen receptor (CAR) vector pS-5.28.z-IEW to target ErbB2-positive tumors. Solely CIK cell subsets with the CD3+ T cell phenotype showed up to 85% cell surface expression of the respective CAR. A comparison of wildtype (WT), mock-vector and ErbB2-CAR-CIK cells showed, that engineered cells exhibited diminished in vitro expansion, retained WT CIK cell phenotype with higher percentages of differentiated effector memory/effector cells. Activating natural killer (NK) cell receptor NKG2D-restricted target cell recognition and killing of WT and ErbB2-CAR-CIK cells was maintained against ErbB2-negative tumors, while ErbB2-CAR-CIK cells demonstrated significantly increased cytotoxicity against ErbB2-positive targets, including primary tumors. ErbB2-CAR- but not WT CIK cells proliferated, infiltrated and efficiently lysed tumor cell monolayers as well as 3D tumor spheroids.
Here, we demonstrate a potential cell therapeutic approach using ErbB2-CAR-CIK cells for the recognition and elimination of tumor cells expressing ErbB2, which we identified as a targetable antigen on high-risk STS cells.
Abrupt switches between different tenses (past-to-present, present-to-past) are known from oral narratives and medieval literature in Romance languages, but there is little consensus about their function and interpretation. In this study, we combine corpus-linguistic tools with experimental methods and quantitative analysis to shed light on the use of tense switches in a medieval Icelandic prose text (Hrafnkels saga freysgoða). Specifically, we part-of-speech tagged all words in Hrafnkels saga freysgoða and then determined where verbs exhibit tense switches. In a second step, we had 19 subjects mark all parts in the saga they consider climactic so as to study the overall as well as subject-specific correlations between climaxness and tense switches. In the vast majority of subjects, we observe the expected correlation, and for most of these it is significant. We discuss the findings with regard to their implications for tense switching as a performative device and the position of sagas on an orality-literacy continuum.
The article presents in its first part approaches to the concept of text genres and some milestones in the evolution of text linguistics. The aim of this linguistics consists in the research of different ways of constructing texts, regardles of style and genre. Starting from the premise that some textual elements are indices for the text genres, the article exposes some observations regarding delimitations between text genres and research approaches from the intercultural and interdisciplinary perspective.
Risk culture during the last 2000 years - from an aleatory society to the illusion of risk control
(2017)
The culture of risk is 2000 years old, although the term “risk” developed much later. The culture of merchants making decisions under uncertainty and taking the individual responsibility for the uncertain future started with the Roman “Aleatory Society”, continued with medieval sea merchants, who made business “ad risicum et fortunam”, and sustained to the culture of entrepreneurs in times of industrialisation and dynamic economic changes in the 18th and 19th century. For all long-term commercial relationships, the culture of honourable merchants with personal decision-making and individual responsibility worked well. The successful development of sciences, statistics and engineering within the last 100 years led to the conjecture that men can “construct” an economical system with a pre-defined “clockwork” behaviour. Since probability distributions could be calculated ex-post, an illusion to control risk ex-ante became a pattern in business and banking. Based on the recent experiences with the financial crisis, a “risk culture” should understand that human “Strength of Knowledge” is limited and the “unknown unknown” can materialise. As all decisions and all commercial agreements are made under uncertainty, the culture of honourable merchants is key to achieve trust in long-term economic relations with individual responsibility, flexibility to adapt and resilience against the unknown.
Relativistic jets from active galactic nuclei (AGN) often display a non-uniform structure and are, under certain conditions, susceptible to a number of instabilities. An interesting example is the development of non-axisymmetric, Rayleigh-Taylor type instabilities in the case of differentially rotating two-component jets, with the toroidal component of the magnetic field playing a key role in the development or suppression of these instabilities. We have shown that higher magnetization leads to stability against these non-axisymmetric instabilities. Using ray-casting on data from relativistic MHD simulations of two-component jets, we now investigate the effect of these instabilities on the synchrotron emission pattern from the jets. We recover many well known trends from actual observations, e.g., regarding the polarization fraction and the distribution of the position angle of the electric field, in addition to a different emitting region, depending on the stability of the jet.
The terrestrial fern Hypolepis distans (Dennstaedtiaceae) is recorded as indigenous to New Zealand and Tasmania. The fern was not known from New South Wales or the Australian mainland until recently. This paper reports on the recent discovery of the species at Macquarie Pass in the Illawarra area on the south-eastern edge of the Central Tablelands Botanical Subdivision of New South Wales. Its ecology and conservation needs are discussed.
Background: The Aedes (Stegomyia) albopictus subgroup includes 11 cryptic species of which Ae. albopictus is the most widely distributed. Its global expansion associated with a documented vector competence for several emerging arboviruses raise obvious concerns in the recently colonized regions. While several studies have provided important insights regarding medical importance of Ae. albopicus, the investigations of the other sibling species are scarce. In Asia, indigenous populations within the Ae. albopictus subgroup can be found in sympatry. In the present study, we aimed to describe and compare molecular, morphological and bacterial symbionts composition among sympatric individuals from the Ae. albopictus subgroup inhabiting a Vietnamese protected area.
Results: Based on morphological structure of the cibarial armarture, we identified a cryptic species in the forest park at Bù Gia Mập in the south-eastern region of Vietnam. Analysis of nuclear (ITS1-5.8S-ITS2) and mitochondrial (cox1, nad5) markers confirmed the divergence between the cryptic species and Ae. albopictus. Analysis of midgut bacterial microbiota revealed a strong similarity among the two species with a notable difference; contrary to Ae. albopictus, the cryptic species did not harbour any Wolbachia infection.
Conclusions: These results could reflect either a recent invasion of Wolbachia in Ae. albopictus or alternatively a loss of this symbiont in the cryptic species. We argue that neglected species of the Ae. albopictus subgroup are of main importance in order to estimate variation of host-symbionts interactions across evolution.
Aproximadamente cinquenta universidades brasileiras possuem algum tipo de estudo de Língua Alemã, sendo que dezessete delas oferecem a graduação em Letras com habilitação em Alemão, bacharelado ou licenciatura. O objetivo deste trabalho é analisar as crenças e ex-pectativas dos estudantes de Letras/Alemão a respeito do curso que frequentam, a partir da rea-lização de uma investigação em uma universidade pública do Estado do Paraná, em 2016. Com base em um questionário respondido por 18 alunos do Curso de Letras desta instituição, busca-mosidentificar os motivos que os levaram a optar pela licenciatura em Letras/Alemão e suas perspectivas de desenvolvimento profissional. A fundamentação teórica advém da perspectiva dos estudos de crenças discentes (SILVA 2007; BARCELOS2001), mais especificamente dos es-tudos de crenças de alunos acerca da aprendizagem de línguas estrangeiras. A metodologia da pesquisa corresponde a um paradigma híbrido (KUCKARTZ 2014, 2016 e MAYRING 2015), com características de pesquisa qualitativa e quantitativa. Como resultado da pesquisa, entre outros aspectos, observou-se que a maioria dos alunos optou pelo curso de Letras/Alemão como forma de aprender essa língua e no futuro atuarem profissionalmente nessa área, seja no campo da Linguística, da Literatura ou da Tradução.
High precision measurement of the radiative capture cross section of 238U at the n_TOF CERN facility
(2017)
The importance of improving the accuracy on the capture cross-section of 238U has been addressed by the Nuclear Energy Agency, since its uncertainty significantly affects the uncertainties of key design parameters for both fast and thermal nuclear reactors. Within the 7th framework programme ANDES of the European Commission three different measurements have been carried out with the aim of providing the 238U(n,γ) cross-section with an accuracy which varies from 1 to 5%, depending on the energy range. Hereby the final results of the measurement performed at the n_TOF CERN facility in a wide energy range from 1 eV to 700 keV will be presented.
The aim of this work is to provide a precise and accurate measurement of the 238U(n,γ) reaction cross section in the energy region from 1 eV to 700 keV. This reaction is of fundamental importance for the design calculations of nuclear reactors, governing the behavior of the reactor core. In particular, fast reactors, which are experiencing a growing interest for their ability to burn radioactive waste, operate in the high energy region of the neutron spectrum. In this energy region most recent evaluations disagree due to inconsistencies in the existing measurements of up to 15%. In addition, the assessment of nuclear data uncertainty performed for innovative reactor systems shows that the uncertainty in the radiative capture cross section of 238U should be further reduced to 1–3% in the energy region from 20 eV to 25 keV. To this purpose, addressed by the Nuclear Energy Agency as a priority nuclear data need, complementary experiments, one at the GELINA and two at the n_TOF facility, were proposed and carried out within the 7th Framework Project ANDES of the European Commission. The results of one of these 238U(n,γ) measurements performed at the n_TOF CERN facility are presented in this work. The γ-ray cascade following the radiative neutron capture has been detected exploiting a setup of two C6D6 liquid scintillators. Resonance parameters obtained from this work are on average in excellent agreement with the ones reported in evaluated libraries. In the unresolved resonance region, this work yields a cross section in agreement with evaluated libraries up to 80 keV, while for higher energies our results are significantly higher.
Background: Electrical stimulation (ES) has been successfully used to treat bone defects clinically. Recently, both cellular and molecular approaches have demonstrated that ES can change cell behavior such as migration, proliferation and differentiation.
Methods: In the present study we exposed rat bone marrow- (BM-) and adipose tissue- (AT-) derived mesenchymal stem cells (MSCs) to direct current electrical stimulation (DC ES) and assessed temporal changes in osteogenic differentiation. We applied 100 mV/mm of DC ES for 1 h per day for three, seven and 14 days to cells cultivated in osteogenic differentiation medium and assessed viability and calcium deposition at the different time points. In addition, expression of osteogenic genes, Runx2, Osteopontin, and Col1A2 was assessed in BM- and AT-derived MSCs at the different time points.
Results: Results showed that ES changed osteogenic gene expression patterns in both BM- and AT-MSCs, and these changes differed between the two groups. In BM-MSCs, ES caused a significant increase in mRNA levels of Runx2, Osteopontin and Col1A2 at day 7, while in AT-MSCs, the increase in Runx2 and Osteopontin expression were observed after 14 days of ES.
Discussion: This study shows that rat bone marrow- and adipose tissue-derived stem cells react differently to electrical stimuli, an observation that could be important for application of electrical stimulation in tissue engineering.
Der viel diskutierte Begriff der "Multinormativität" steht heute in Konkurrenz zu dem des "Rechtspluralismus" und der "Diversität" des Rechts. Alle drei Begriffe verfügen nicht über eine inhaltliche und funktionale Eindeutigkeit und zeigen kein bestimmtes Verhältnis zu- und untereinander. Eine sehr vage Gemeinsamkeit solcher "Rechtspluralitäten" besteht allein in deren Unterscheidung oder Abweichung vom Gegenbegriff einer "Rechtseinheit" bzw. der ideal gedachten Einheit der Rechtsordnung, wie sie von den Kodifikatoren des Privatrechts im Zeitalter der Aufklärung versucht wurde zu verwirklichen. In einem solchen Spannungsverhältnis zwischen Einheitlichkeit oder Vielfalt gesellschaftlicher und staatlicher Rechtsgestaltung steht jedes organisierte Gemeinwesen. Das ist eine historische Erfahrung, die sich seit der römischen Antike in der rechtssystematischen Einteilung in ein "ius universale " und "ius speciale/particulare" zeigt. In der aktuellen Diskussion scheint "Multinormativität" als der umfassendere Begriff für die Rechtevielfalt gebraucht zu werden. In der rechtstheoretisch orientierten Literatur kann man je nach disziplinärer Einteilung bis zu sieben "Rechtspluralismen" unterscheiden, die die Definitionsnot und sprachliche Bedeutungsvielfalt nachdrücklich belegen. Dieser "Pluralismus" kann durch Rechte oder auch "Rechts-Ordnungen" in der Interaktion zwischen einer herrschenden und einer alternativen "Rechts-Ordnung" bestimmt sein. Die Konkretisierungsbedürftigkeit von "Rechtspluralismus" und "Multinormativität" haben diese beiden Begriffe auch gemeinsam mit dem geläufigen Nachbarbegriff der pluralen "Rechtsquellen", die als Sammelbezeichnung für die Grundlagen rechtlicher Entscheidungen fungieren. Multinormativität kann heute als eine extensive Variante von "Rechtsquellenvielfalt" oder "Rechtsvielfalt" gesehen werden, beschränkt sich jedoch nicht auf die Dimension der rechtlichen Entscheidungsgrundlagen, sondern erweitert diese auch auf die Vielzahl möglicher außerrechtlicher entscheidungsrelevanter "Normen". Das ist der Grund, weshalb heute der Begriff des "normative pluralism" dem des "legal pluralism" vorgezogen wird, um auch das "phenomenon of law beyond the state" in den Griff zu bekommen. In der deutschen Literatur ist heute das beliebte Bild und der vielgebrauchte Begriff der "Rechtsquellen" höchst umstritten, da in ihm mehr das "fließende" Element höchst mobiler rechtlicher Ordnungsinstrumente zum Ausdruck kommt als die Bestimmtheit und Sicherheit rechtlicher Normangebote. Luhmann bezeichnete die eine Rechtspluralität indizierende Rechtsquellenlehre als ein "mehrschichtiges Theoriegebäude mit schwankenden Fundamenten" und Esser sprach sogar von den "doktrinären Peinlichkeiten", vor die sich die Rechtsquellenlehre gestellt sah und gestellt sieht. Multinormativität löst heute weitgehend den "Rechtsquellen"-Begriff ab, indem er ihn vor dem Hintergrund internationaler politischer Praxis und Erfahrungen überlagert und ausweitet. ...
The transcription factor Meis1 drives myeloid leukemogenesis in the context of Hox gene overexpression but is currently considered undruggable. We therefore investigated whether myeloid progenitor cells transformed by Hoxa9 and Meis1 become addicted to targetable signaling pathways. A comprehensive (phospho)proteomic analysis revealed that Meis1 increased Syk protein expression and activity. Syk upregulation occurs through a Meis1-dependent feedback loop. By dissecting this loop, we show that Syk is a direct target of miR-146a, whose expression is indirectly regulated by Meis1 through the transcription factor PU.1. In the context of Hoxa9 overexpression, Syk signaling induces Meis1, recapitulating several leukemogenic features of Hoxa9/Meis1-driven leukemia. Finally, Syk inhibition disrupts the identified regulatory loop, prolonging survival of mice with Hoxa9/Meis1-driven leukemia.
The challenges posed by climate and land use change are increasingly complex, with ever-increasing and accelerating impacts on the global environmental system. The establishment of an internationally harmonized, integrated, and long-term operated environmental monitoring infrastructure is one of the major challenges of modern environmental research. Increased efforts are currently being made in Europe to establish such a harmonized pan-European observation infrastructure, and the European network of Long-Term Ecological Research sites – LTER-Europe – is of particular importance. By evaluating 477 formally accredited LTER-Europe sites, this study gives an overview of the current distribution of these infrastructures and the present condition of long-term environmental research in Europe. We compiled information on long-term biotic and abiotic observations and measurements and examined the representativeness in terms of continental biogeographical and socio-ecological gradients. The results were used to identify gaps in both measurements and coverage of the aforementioned gradients. Furthermore, an overview of the current state of the LTER-Europe observation strategies is given. The latter forms the basis for investigating the comparability of existing LTER-Europe monitoring concepts both in terms of observational design as well as in terms of the scope of the environmental compartments, variables and properties covered.
In diesem Beitrag wird korpusbasiert, qualitativ und aus kontrastiver Sicht anhand ausgewählter Einzelbeispiele untersucht, welchen Beitrag die Frame-Semantik bei der Analyse konzeptueller Metaphern im aktuellen Migrationsdiskurs in der deutschen und italienischen Presse leisten kann. Sie macht es möglich, unterschiedliche Perspektivierungen bei der Betrachtung des Phänomens herauszukristallisieren, da semantische Frames ein facettenreiches Angebot für den Blickwinkel der Betrachtung bereitstellen. Auch Metaphern selbst besitzen diese Eigenschaft der Perspektivierung, vor allem auch solche, die charakteristisch für einen kulturspezifischen Kontext stehen. Zu den Möglichkeiten, die der Einsatz von Metaphern bietet, zählt vor allem der "Highlighting und Hiding"-Effekt (LAKOFF/JOHNSON 1980), indem Metaphern bestimmte Aspekte betonen oder verdunkeln. Um welche es sich dabei im Einzelnen handelt, hängt von mehreren Faktoren ab, wie zum Beispiel der politischen Orientierung von Journalisten oder Politikern oder der wirtschaftlichen Lage eines Landes.
In all eukaryotic cells, the nucleolus is functionally and structurally linked to rRNA synthesis and ribosome biogenesis. This compartment contains as well factors involved in other cellular activities, but the functional interconnection between non-ribosomal activities and the nucleolus (structure and function) still remains an open question. Here, we report a novel mass spectrometry analysis of isolated nucleoli from Arabidopsis thaliana plants using the FANoS (Fluorescence Assisted Nucleolus Sorting) strategy. We identified many ribosome biogenesis factors (RBF) and proteins non-related with ribosome biogenesis, in agreement with the recognized multi-functionality of the nucleolus. Interestingly, we found that 26S proteasome subunits localize in the nucleolus and demonstrated that proteasome activity and nucleolus organization are intimately linked to each other. Proteasome subunits form discrete foci in the disorganized nucleolus of nuc1.2 plants. Nuc1.2 protein extracts display reduced proteasome activity in vitro compared to WT protein extracts. Remarkably, proteasome activity in nuc1.2 is similar to proteasome activity in WT plants treated with proteasome inhibitors (MG132 or ALLN). Finally, we show that MG132 treatment induces disruption of nucleolar structures in WT but not in nuc1.2 plants. Altogether, our data suggest a functional interconnection between nucleolus structure and proteasome activity.
O intuito deste trabalho é destacar a importância do escrito juvenil Traumland para a formação do poeta Georg Trakl, por meio de uma leitura cerrada do texto. Sob a forma de uma lembrança do narrador, esse breve relato apresenta uma experiência de iniciação ou perda da inocência oriunda da percepção do mal no mundo, concentrado na figura de uma prima doente, Maria. O conflito entre o anseio romântico (Sehnsucht) pleno de expectativas e entusiasmo e essa realidade aterradora, vivenciado como uma quebra no sentimento (Stimmung) de harmonia cósmica, impulsiona uma transformação no sentimento e na visão de mundo do protagonista, que metaforicamente pode ser lida como a transição de uma poética puramente romântica para uma poética mais sintonizada com as exigências do século XX, especialmente no sentido da dimensão reconhecida ao mal irremediável, que não pode ser completamente romantizado.
Kaum eine geistes- und kulturwissenschaftliche Disziplin hat in Italien in solchem Ausmaß unter Selbstdarstellungs- und Rechtfertigungszwang gestanden und sich über so heftige Widerstände hinwegsetzen müssen wie die Komparatistik. Ein Leserbrief des Literaturwissenschaftlers Armando Gnisci, der in den 1980er- und 1990er-Jahren die Szene der italienischen Komparatistik wesentlich geprägt, aber nur marginal bis ins neue Jahrtausend mitbestimmen konnte, gibt einen konzis-kritischen – wenn auch leicht polemischen – Einblick in das, was seit dem letzten state of the art aus dem Jahre 1991 auf dem Gebiet der Allgemeinen und Vergleichenden Literaturwissenschaft in Italien passiert ist. Entlang dieser Linie verlief nämlich die wichtigste Front im Streit zwischen den Nationalphilologen und den Komparatisten. Gniscis Schreiben mit dem programmatischen wie suggestiven Titel "Comparatisti e italianisti, le due tribù" [Komparatisten und Italianisten, die beiden Stämme] – erschienen am 21. März 2004 in der Kulturbeilage Domenica – ist ein Rundumschlag gegen die seinerzeit in Italien weitverbreitete Ansicht, die Allgemeine und Vergleichende Literaturwissenschaft als "sub-category of Italian literature departments" zu betrachten.
We study platform design in online markets in which buying involves a (nonmonetary) cost for consumers caused by privacy and security concerns. Firms decide whether to require registration at their website before consumers learn relevant product information. We derive conditions under which a monopoly seller benefits from ex ante registration requirements and demonstrate that the profitability of registration requirements is increased when taking into account the prospect of future purchases or an informational value of consumer registration to the firm. Moreover, we consider the effectiveness of discounts (store credit) as a means to influence the consumers’ registration decision. Finally, we confirm the profitability of ex ante registration requirements in the presence of price competition.
We study the effect of the chiral symmetry restoration (CSR) on heavy-ion collisions observables in the energy range sNN=3–20GeV within the Parton-Hadron-String Dynamics (PHSD) transport approach. The PHSD includes the deconfinement phase transition as well as essential aspects of CSR in the dense and hot hadronic medium, which are incorporated in the Schwinger mechanism for particle production. Our systematic studies show that chiral symmetry restoration plays a crucial role in the description of heavy-ion collisions at sNN=3–20GeV, realizing an increase of the hadronic particle production in the strangeness sector with respect to the non-strange one. Our results provide a microscopic explanation for the horn structure in the excitation function of the K+/π+ ratio: the CSR in the hadronic phase produces the steep increase of this particle ratio up to sNN≈7GeV, while the drop at higher energies is associated to the appearance of a deconfined partonic medium. Furthermore, the appearance/disappearance of the horn structure is investigated as a function of the system size. We additionally present an analysis of strangeness production in the (T,μB)-plane (as extracted from the PHSD for central Au+Au collisions) and discuss the perspectives to identify a possible critical point in the phase diagram.
Protein disulfide isomerases (PDIs) support endoplasmic reticulum redox protein folding and cell-surface thiol-redox control of thrombosis and vascular remodeling. The family prototype PDIA1 regulates NADPH oxidase signaling and cytoskeleton organization, however the related underlying mechanisms are unclear. Here we show that genes encoding human PDIA1 and its two paralogs PDIA8 and PDIA2 are each flanked by genes encoding Rho guanine-dissociation inhibitors (GDI), known regulators of RhoGTPases/cytoskeleton. Evolutionary histories of these three microsyntenic regions reveal their emergence by two successive duplication events of a primordial gene pair in the last common vertebrate ancestor. The arrangement, however, is substantially older, detectable in echinoderms, nematodes, and cnidarians. Thus, PDI/RhoGDI pairing in the same transcription orientation emerged early in animal evolution and has been largely maintained. PDI/RhoGDI pairs are embedded into conserved genomic regions displaying common cis-regulatory elements. Analysis of gene expression datasets supports evidence for PDI/RhoGDI coexpression in developmental/inflammatory contexts. PDIA1/RhoGDIα were co-induced in endothelial cells upon CRISP-R-promoted transcription activation of each pair component, and also in mouse arterial intima during flow-induced remodeling. We provide evidence for physical interaction between both proteins. These data support strong functional links between PDI and RhoGDI families, which likely maintained PDI/RhoGDI microsynteny along > 800-million years of evolution.
The paper presents the possibilities for analyzing deverbal derivatives from verbs ending in -ier(en); in many cases these exist as doublets, with the suffix -ung and/or the suffix -(at)ion. Based on selected examples of these doublets, the paper demonstrates how both monolingual and bilingual dictionaries fail to take sufficient account of the semantic differences between such competing forms. The author also presents research questions connected with this issue, and outlines a methodology for addressing these questions based on metalexicographic and corpus analysis; this methodology is demonstrated using the example of the doublet Diskrimination and Diskriminierung
We have determined the crystal structures of two decachlorocyclopentasilanes, namely bis(tetra-n-butylammonium) dichloride decachlorocyclopentasilane dichloromethane disolvate, 2C16H36N+·2Cl−·Si5Cl10·2CH2Cl2, (I), and bis(tetraethylammonium) dichloride decachlorocyclopentasilane dichloromethane disolvate, 2C8H20N+·2Cl−·Si5Cl10·2CH2Cl2, (II), both of which crystallize with discrete cations, anions, and solvent molecules. In (I), the complete decachlorocyclopentasilane ring is generated by a crystallographic twofold rotation axis. In (II), one cation is located on a general position and the other two are disordered about centres of inversion. These are the first structures featuring the structural motif of a five-membered cyclopentasilane ring coordinated from both sides by a chloride ion. The extended structures of (I) and (II) feature numerous C—H⋯Cl interactions. In (II), the N atoms are located on centres of inversion and as a result, the ethylene chains are disordered over equally occupied orientations.
Rho GTPases control fundamental cellular processes and Cdc42 is a well-studied member of the family that controls filopodia formation and cell migration. Although the regulation of Cdc42 activity by nucleotide binding is well documented, the mechanisms driving its proteostasis are not clear. Here, we demonstrate that the highly conserved, RING domain containing E3 ubiquitin ligase XIAP controls the protein stability of Cdc42. XIAP binds to Cdc42 and directly conjugates poly ubiquitin chains to the Lysine 166 of Cdc42 targeting it for proteasomal degradation. Depletion of XIAP led to an increased protein stability and activity of Cdc42 in normal and tumor cells. Consistently, loss of XIAP enhances filopodia formation in a Cdc42-dependent manner and this phenomenon phenocopies EGF stimulation. Further, XIAP depletion promotes lung colonization of tumor cells in mice in a Cdc42-dependent manner. These observations shed molecular insights into ubiquitin-dependent regulation of Cdc42 and that of actin cytoskeleton.
The value of plant ecological datasets with hundreds or thousands of species is principally determined by the taxonomic accuracy of their plant names. However, combining existing lists of species to assemble a harmonized dataset that is clean of taxonomic errors can be a difficult task for non-taxonomists. Here, we describe the range of taxonomic difficulties likely to be encountered during dataset assembly and present an easy-to-use taxonomic cleaning protocol aimed at assisting researchers not familiar with the finer details of taxonomic cleaning. The protocol produces a final dataset (FD) linked to a companion dataset (CD), providing clear details of the path from existing lists to the FD taken by each cleaned taxon. Taxa are checked off against ten categories in the CD that succinctly summarize all taxonomic modifications required. Two older, publicly-available lists of naturalized Asteraceae in Australia were merged into a harmonized dataset as a case study to quantify the impacts of ignoring the critical process of taxonomic cleaning in invasion ecology. Our FD of naturalized Asteraceae contained 257 species and infra-species. Without implementation of the full cleaning protocol, the dataset would have contained 328 taxa, a 28% overestimate of taxon richness by 71 taxa. Our naturalized Asteraceae CD described the exclusion of 88 names due to nomenclatural issues (e.g. synonymy), the inclusion of 26 updated currently accepted names and four taxa newly naturalized since the production of the source datasets, and the exclusion of 13 taxa that were either found not to be in Australia or were in fact doubtfully naturalized. This study also supports the notion that automated processes alone will not be enough to ensure taxonomically clean datasets, and that manual scrutiny of data is essential. In the long term, this will best be supported by increased investment in taxonomy and botany in university curricula.
Erasmus of Rotterdam was a scholar with a broad range of interests living in the Renaissance and Reformation period, and he has become the eponymous figurehead of a European university mobility programme initiated thirty years ago. With reference to the partnership between the University of Ostrava (Czech Republic) and the Zittau/Görlitz University of Applied Sciences (Germany), this paper demonstrates the key importance of this programme for university courses in translation studies, showing how the programme is implemented in line with Erasmus of Rotterdam's ideas and convictions.
OBJECTIVE: The role of supraglottic airway devices in emergency airway management is highlighted in international airway management guidelines. We evaluated the application of the new generation laryngeal tube suction (LTS-II/LTS-D) in the management of in-hospital unexpected difficult airway and cardiopulmonary resuscitation.
METHODS: During a seven-year period, patients treated with a laryngeal tube who received routine anesthesia and had an unexpected difficult airway (Cormack Lehane Grade 3-4), who underwent cardiopulmonary resuscitation, or who underwent cardiopulmonary resuscitation outside the operating room and had a difficult airway were evaluated. Successful placement of the LTS II/LTS-D, sufficient ventilation, time to placement, number of placement attempts, stomach content, peripheral oxygen saturation/end-tidal carbon dioxide development (SpO2/etCO2) over 5 minutes, subjective overall assessment and complications were recorded.
RESULTS: In total, 106 adult patients were treated using an LTS-II/LTS-D. The main indication for placement was a difficult airway (75%, n=80), followed by cardiopulmonary resuscitation (25%, n=26) or an overlap between both (18%, n=19). In 94% of patients (n=100), users placed the laryngeal tube during the first attempt. In 93% of patients (n=98), the tube was placed within 30 seconds. A significant increase in SpO2 from 97% (0-100) to 99% (5-100) was observed in the whole population and in cardiopulmonary resuscitation patients. The average initial etCO2 of 39.5 mmHg (0-100 mmHg) decreased significantly to an average of 38.4 mmHg (10-62 mmHg) after 5 minutes. A comparison of cardiopulmonary resuscitation patients with non-cardiopulmonary resuscitation patients regarding gastric contents showed no significant difference.
CONCLUSIONS: LTS-D/LTS-II use for in-hospital unexpected difficult airway management provides a secure method for primary airway management until other options such as video laryngoscopy or fiber optic intubation become available.
Mutualistic interactions between plants and animals can affect both plant and animal communities, and potentially leave imprints on plant demography. Yet, no study has simultaneously tested how trait variation in plant resources shapes the diversity of animal consumers, and how these interactions influence seedling recruitment. Here, we analyzed whether (i) phylogenetic diversity and functional diversity of fruiting plants were correlated with the corresponding diversity of frugivorous birds, and (ii) whether phylogenetic diversity and functional identity of plant and bird communities influenced the corresponding diversity and identity of seedling communities. We recorded mutualistic interactions between fleshy-fruited plants and frugivorous birds and seedling communities in 10 plots along an elevational gradient in the Colombian Andes. We built a phylogeny for plants/seedlings and birds and measured relevant morphological plant and bird traits that influence plant-bird interactions and seedling recruitment. We found that phylogenetic diversity and functional diversity of frugivorous birds were positively associated with the corresponding diversities of fruiting plants, consistent with a bottom-up effect of plants on birds. Moreover, the phylogenetic diversity of seedlings was related to the phylogenetic diversity of plants, but was unrelated to the phylogenetic diversity of frugivorous birds, suggesting that top-down effects of animals on seedlings were weak. Mean seed mass of seedling communities was positively associated with the mean fruit mass of plants, but was not associated with the mean avian body mass in the frugivore communities. Our study shows that variation in the traits of fleshy-fruited plants was associated with the diversity of frugivorous birds and affected the future trajectory of seedling recruitment, whereas the morphological traits of animal seed dispersers were unrelated to the phylogenetic and functional structure of seedling communities. These findings suggest that bottom-up effects are more important than top-down effects for seed-dispersal interactions and seedling recruitment in diverse tropical communities.
Bestimmung des klinischen Nutzens systemischer adjuvanter Therapien beim frühen Mammakarzinom
(2017)
Die onkologische Therapie befindet sich im Umbruch. Hohe Erwartungen sind mit einer Reihe innovativer zielgerichteter Medikamente verknüpft, die sich derzeit in der klinischen Entwicklung befinden. Vor diesem Hintergrund erfahren Diskussionen um die Begriffe klinischer Nutzen oder klinische Relevanz neue Aktualität. Dies gilt auch für die Weiterentwicklungen der adjuvanten systemischen Therapie des frühen Mammakarzinoms. In Anbetracht der kurativen Zielsetzung erfolgt die Beurteilung des klinischen Nutzens einer adjuvanten Therapie maßgeblich anhand von Wirksamkeitsendpunkten. Der Fokus liegt hierbei auf Verbesserungen des krankheitsfreien Überlebens und des Rezidivrisikos. Eine Aussage zum Gesamtüberleben ist aufgrund der heute erreichten niedrigen Mortalitätsraten erst nach sehr langen Beobachtungszeiten möglich. Folgerichtig sollte neuen Medikamenten für die adjuvante Therapie ein klinischer Nutzen zugesprochen werden, wenn sie eine weitere Reduktion des Rezidivrisikos über den heutigen hohen Standard hinaus ermöglichen. Die Evidenz für etablierte adjuvante Therapiestandards beim frühen Mammakarzinom kann als objektiver Maßstab zum Vergleich herangezogen werden. Am Beispiel der adjuvanten endokrinen Therapie, der adjuvanten Polychemotherapie und der adjuvanten Anti-HER2-Therapie werden in diesem Übersichtsartikel die Anforderungen für den klinischen Nutzen neuer adjuvanter Therapien beim frühen Mammakarzinom abgeleitet.
Oncologic therapy is currently undergoing significant changes. A number of innovative targeted medications currently in clinical development have raised high expectations. With that in mind, discussions about terms such as "clinical benefit" and "clinical relevance" are highly topical. This also applies to further developments in the field of adjuvant systemic therapies for early-stage breast cancer. As the treatment aim is curative, assessment of the clinical benefit of adjuvant therapies must be largely based on efficacy outcomes. The focus must be on improving disease-free survival rates and lowering the risk of recurrence. Because of the current low mortality rates, statements about overall survival rates are only possible after very long observation periods. Consequently, new drugs in adjuvant therapies should be considered as offering a clinical benefit, if they reduce the risk of recurrence below current low levels of risk. The evidence for established adjuvant therapy standards in early-stage breast cancer can be used as objective criteria for comparison. This review article considers the requirements for clinical benefit of new adjuvant therapies for early breast cancer, based on examples from adjuvant endocrine therapy, adjuvant polychemotherapy and adjuvant anti-HER2 therapy.
Diskursive Praktiken, insbesondere Texte, bewegen sich in einem kulturellen Feld, das in raumtheoretischer Metaphorisierung als von der Trias Zentrum- Peripherie-Außenraum konstituiertes chronotopisches Feld bezeichnet werden kann, mindestens bi-, wahrscheinlich multidirektional und in unterschiedlichen Geschwindigkeiten zwischen seinen beiden Polen verlaufend. Der in den Theologien etablierte Begriff des 'Apokryphen' ist demgegenüber kaum zufällig schon in seiner Semantik statisch und vermag nur die Situierung einer Schrift oder einer Wissenstradition 'im Verborgenen', nicht hingegen ihre Bewegung in Richtung dieses Bereichs, zu thematisieren. Dahinter steht bekanntlich das Anliegen einer Strukturierung epistemischer Bestände mit dem Ziel der Kanonisierung und Gewinnung von Autorität in der (im weitesten Sinne) gesellschaftlichen Kommunikation. Tatsächlich bildet das Konzept von kanonischer und apokrypher Literatur, nimmt man die Kategorie verbotener, indizierter Bücher im Sinne eines diskursiven Außenraums hinzu, auch die oben genannte Trias ab. Denn als 'Apokryphen' lassen sich dann Texte bezeichnen, die nicht kanonisch geworden sind, obwohl ihre inhaltlichen Potentiale das nicht a priori ausgeschlossen hätten. Apokryphe Texte stehen demnach in der diskursiven Peripherie. Grundsätzlich haben sie die Chance, zu einem ferneren Zeitpunkt kanonisch zu werden, sie könnten aber auch zu einer Existenz im Verbotenen verdammt werden.
Die deutschsprachige ästhetische und kulturtheoretisch-anthropologische Debatte des ausgehenden 18. Jahrhunderts thematisiert in unterschiedlichsten Kontexten immer wieder den Umgang mit Fremdheitserfahrung. Sie umfasst in diesem Zusammenhang unterschiedlichste theoretische Modelle der prekären Vermittlung zwischen gegensätzlichen Polen wie besonderer Einzelerfahrung und allgemeinem Erfahrungsganzem, zwischen Einzelphänomen und Kontext, zwischen Fragment und Totalität oder zwischen Singularität und Universalität. Entsprechende Fragestellungen rücken in der Zeit der Spätaufklärung vor allem mit Blick auf die Vermittlung (inter)kultureller Fremdheit in den Fokus des theoretischen und literarischen Interesses: Dies gilt vor allem für die Kulturpraxis inner- und außereuropäischer Reisen, die im Rahmen der zeitgenössischen 'Reisemode' zu Debatten über die Möglichkeit kosmopolitischen Weltbürgertums, über Modi interkultureller Begegnung oder die Legitimität kolonialer Expansion ebenso wie zur Entwicklung einer konkreten ethnopoetischen Reise- und Reisedarstellungspoetik Anlass gibt. Dieser philosophisch-theoretischen und literarischen Konjunktur von Reisediskursen korrespondieren gleichzeitig unterschiedliche Entwürfe einer transnationalen vergleichenden Kulturgeschichte, die immer wieder in kulturelle Hierarchisierungsmodelle münden, in denen europäischen Kulturen erwartungsgemäß eine wenn nicht qualitative oder normative, so doch immerhin strategische Überlegenheit zuerkannt wird. Hiermit verbinden sich drittens frühe Ansätze zur Theorie und Praxis 'weltliterarischer' Bildung sowie Appelle für eine grenzüberschreitende Beschäftigung mit literarischen und kulturellen Artefakten als Vorläufermodelle komparatistischer Literatur- und Kulturwissenschaft.
Background/Aims: Alcohol (ethanol, EtOH) as significant contributor to traumatic injury is linked to suppressed inflammatory response, thereby influencing clinical outcomes. Alcohol-induced immune-suppression during acute inflammation (trauma) was linked to nuclear factor-kappaB (NF-ĸB). Here, we analyzed alcohol`s effects and mechanisms underlying its influence on NF-ĸB-signaling during acute inflammation in human lung epithelial cells. Methods: A549-cells were stimulated with interleukin (IL)-1β, or sera from trauma patients (TP) or healthy volunteers, with positive/negative blood alcohol concentrations (BAC), and subsequently exposed to EtOH (170 Mm, 1h). IL-6-release and neutrophil adhesion to A549 were analyzed. Specific siRNA-NIK mediated downregulation of non-canonical, and IKK-NBD-inhibition of canonical NF-ĸB signaling were performed. Nuclear levels of activated p50 and p52 NF-ĸB-subunits were detected using TransAm ELISA. Results: Both stimuli significantly induced IL-6-release (39.79±4.70 vs. 0.58±0.8 pg/ml) and neutrophil adhesion (132.30±8.80 vs. 100% control, p<0.05) to A549-cells. EtOH significantly decreased IL-6-release (22.90±5.40, p<0.05) and neutrophil adherence vs. controls (105.40±14.5%, p<0.05). IL-1β-induced significant activation of canonical/p50 and non-canonical/p52 pathways. EtOH significantly reduced p50 (34.90±23.70 vs. 197.70±36.43, p<0.05) not p52 activation. Inhibition of canonical pathway was further increased by EtOH (less p50-activation), while p52 remained unaltered. Inhibition of non-canonical pathway was unchanged by EtOH. Conclusion: Here, alcohol`s anti-inflammatory effects are mediated via decreasing nuclear levels of activated p50-subunit and canonical NF-ĸB signaling pathway.
The KER for electron capture of vibrational cooled HeH+ and H3 + ions at 20 keV from residual gas atoms has been measured in the Frankfurt Low Energy Storage Ring (FLSR). At a vacuum in the order of few 10-11 mbar, this residual gas consists to 99% of H2 molecules. For the identification of the recoil products of this reaction, a recoil spectrometer (with an MCP-detector with position and time sensitive read out) was installed at one of the focus points (IP) in the FLSR. The planned extension of this set up by a gas target to a full COLTRIMS reaction microscope will be discussed.
Some anaerobic archaea and bacteria live on substrates that do not allow the synthesis of one mol of ATP per mol of substrate via substrate level phosphorylation (SLP). Energy conservation in these cases is only possible by a chemiosmotic mechanism that involves the generation of an electrochemical ion gradient across the cytoplasmic membrane that then drives ATP synthesis via an ATP synthase. The minimal amount of energy required for ATP synthesis is thus dependent on the magnitude of the electrochemical ion gradient, the phosphorylation potential in the cell and the ion/ATP ratio of the ATP synthase. It was always thought that the minimum biological energy quantum is defined as the amount of energy required to translocate one ion across the cytoplasmic membrane. We will discuss the thermodynamics of the reactions involved in chemiosmosis and describe the limitations for ion transport and ATP synthesis that led to the proposal that at least −20 kJ/mol are required for ATP synthesis. We will challenge this hypothesis by arguing that the enzyme energizing the membrane may translocate net less than one ion: By using a primary pump connected to an antiporter module a stoichiometry below one can be obtained, implying that the minimum biological energy quantum that sustains life is even lower than assumed to date.
Objectives: There is promising evidence that mindfulness-based interventions are effective in reducing the symptoms of posttraumatic stress disorder (PTSD). However, until now, studies have often lacked a full clinical PTSD assessment, and interventions are often administered in addition to other interventions. This study examined the feasibility of mindfulness-based stress reduction (MBSR) as a standalone intervention in patients with PTSD who have experienced mixed traumatic events.
Method: Fourteen patients participated in 8 weeks of MBSR. The patients were assessed prior to treatment, post-treatment and at a 1-month follow-up through self-ratings (e.g., the Davidson Trauma Scale) and the Clinician-Administered PTSD Scale to determine the effects of the intervention. Furthermore, after the intervention, the patients participated in qualitative interviews regarding their experiences with MBSR and their ideas for future improvements.
Results: Nine patients finished the program, and these patients considered the exercises to be applicable and helpful. In the Clinician-Administered PTSD Scale, we found large effects regarding the reduction of PTSD symptoms among completers (Cohen's d = 1.2). In the Davidson Trauma Scale, the effect sizes were somewhat lower (Cohen's d = 0.6) but nevertheless confirmed the efficacy of MBSR in reducing PTSD symptoms. In the qualitative interviews, the patients reported an augmentation of wellbeing and improvement regarding the handling of difficult situations and more distance from the traumatic event.
Conclusion: Despite the large effects, the high dropout rates and the results of the post-treatment interviews suggest that the intervention should be better adapted to the needs of PTSD patients, e.g., by giving more information regarding the exercises and by including shorter exercises to manage acute distress.
Internationale Bibliographie der Sekundärliteratur zu Bertha von Suttner. Stand: Juni 2017. - Nicht aufgenommen wurden Rezensionen, Zeitungsartikel, Romanbiographien, Dramatisierungen von Leben und Romanen, Filmbiographien, Filmdokumentationen, einzelne Kapitel in übergreifenden Publikationen zum Nobelpreis, (Foto-)Portraits.
Background and Purpose: This review aims to provide a comprehensive overview of the literature and elucidate open questions for future clinical trials concerning diagnostics and treatment modalities for cervical cancer of unknown primary (CUP).
Methods: A literature search for head and neck CUP was performed with focus on diagnostics and therapies as well as molecular markers.
Results: High level evidence on CUP is limited. However, it seems that a consensus exists regarding the optimal diagnostic procedures. The correct implementation of biomarkers for patient stratification and treatment remains unclear. An even greater dispute dominates about the ideal treatment with publications ranging from sole surgery to surgery with postoperative bilateral radiotherapy with inclusion of the mucosa and concomitant chemotherapy.
Conclusions: Cervical CUP represents a very heterogeneous malignant disease. On this account many aspects concerning treatment optimization remain unclear, despite a considerable number of publications in the past. Future research in form of prospective randomized trials is needed in order to better define patient stratification criteria and enable tailored treatment.
Nucleation modeling of the Antarctic stratospheric CN layer and derivation of sulfuric acid profiles
(2017)
Recent analysis of long-term balloon-borne measurements of Antarctic stratospheric condensation nuclei (CN) between July and October showed the formation of a volatile CN layer at 21–27 km altitude in a background of existing particles. We use the nucleation model SAWNUC to simulate these CN in subsiding air parcels and study their nucleation and coagulation characteristics. Our simulations confirm recent analysis that the development of the CN layer can be explained with neutral sulfuric acid–water nucleation and we show that outside the CN layer the measured CN concentrations are well reproduced just considering coagulation and the subsidence of the air parcels. While ion-induced nucleation is expected as the dominating formation process at higher temperatures, it does not play a significant role during the CN layer formation as the charged clusters recombine too fast. Further, we derive sulfuric acid concentrations for the CN layer formation. Our concentrations are about 1 order of magnitude higher than previously presented concentrations as our simulations consider that nucleated clusters have to grow to CN size and can coagulate with preexisting particles. Finally, we calculate threshold sulfuric acid profiles that show which concentration of sulfuric acid is necessary for nucleation and growth to observable size. These threshold profiles should represent upper limits of the actual sulfuric acid outside the CN layer. According to our profiles, sulfuric acid concentrations seem to be below midlatitude average during Antarctic winter but above midlatitude average for the CN layer formation.
Background: As members of the Notothenioidei - the dominant fish taxon in Antarctic waters - the family Bathydraconidae includes 12 genera and 17 species. The knowledge of these species inhabiting an isolated environment is rather fragmentary, including their parasite fauna. Studies on fish hosts and their associated parasites can help gain insights into even remote ecosystems and be used to infer ecological roles in food webs; however, ecological studies on the Bathydraconidae are scarce.
Results: In this study, stomach contents and parasite fauna of the Antarctic dragonfish species Parachaenichthys charcoti (n = 47 specimens) as well as of Gerlachea australis (n = 5), Gymnodraco acuticeps (n = 9) and Racovitzia glacialis (n = 6) were examined. The parasite fauna of P. charcoti consisted of eight genera represented by 11 species, with three of them being new host records. Overall, 24 parasite genera and 26 species were found in the sampled fish, including eleven new host records.
Conclusion: Analyses revealed that the majority of the parasite species found in the different fish hosts are endemic to Antarctic waters and are characterized by a broad host range. These findings are evidence for the current lack of knowledge and the need for further parasitological studies of fish species in this unique habitat.
We study the sensitivities of the directed flow in Au+Au collisions on the equation of state (EoS), employing the transport theoretical model JAM. The EoS is modified by introducing a new collision term in order to control the pressure of a system by appropriately selecting an azimuthal angle in two-body collisions according to a given EoS. It is shown that this approach is an efficient method to modify the EoS in a transport model. The beam energy dependence of the directed flow of protons is examined with two different EoS, a first-order phase transition and crossover. It is found that our approach yields quite similar results as hydrodynamical predictions on the beam energy dependence of the directed flow; Transport theory predicts a minimum in the excitation function of the slope of proton directed flow and does indeed yield negative directed flow, if the EoS with a first-order phase transition is employed. Our result strongly suggests that the highest sensitivity for the critical point can be seen in the beam energy range of 4.7 ≤√sNN≤11.5GeV.
Background: Correct species identification of blow flies is a crucial step for understanding their biology, which can be used not only for designing fly control programs, but also to determine the minimum time since death. Identification techniques are usually based on morphological and molecular characters. However, the use of classical morphology requires experienced entomologists for correct identification; while molecular techniques rely on a sound laboratory expertise and remain ambiguous for certain taxa. Landmark-based geometric morphometric analysis of insect wings has been extensively applied in species identification. However, few wing morphometric analyses of blow fly species have been published.
Methods: We applied a landmark-based geometric morphometric analysis of wings for species identification of 12 medically and forensically important blow fly species of Thailand. Nineteen landmarks of each right wing of 372 specimens were digitised. Variation in wing size and wing shape was analysed and evaluated for allometric effects. The latter confirmed the influence of size on the shape differences between species and sexes. Wing shape variation among genera and species were analysed using canonical variates analysis followed by a cross-validation test.
Results: Wing size was not suitable for species discrimination, whereas wing shape can be a useful tool to separate taxa on both, genus and species level depending on the analysed taxa. It appeared to be highly reliable, especially for classifying Chrysomya species, but less robust for a species discrimination in the genera Lucilia and Hemipyrellia. Allometry did not affect species separation but had an impact on sexual shape dimorphism.
Conclusions: A landmark-based geometric morphometric analysis of wings is a useful additional method for species discrimination. It is a simple, reliable and inexpensive method, but it can be time-consuming locating the landmarks for a large scale study and requires non-damaged wings for analysis.
In einem neueren Sammelband der Schnitzler-Forschung ist der Abschnitt, unter dem Beiträge zum Zusammenhang von Arthur Schnitzlers Werk mit der Psychologie versammelt sind, überschrieben mit: "Einmal noch: Psychoanalyse". Was, so mag man fragen, kann es nach fast hundertjähriger, intensiver Forschung zu Schnitzler und der Psychologie noch Neues zu diesem Thema zu sagen geben - und entsprechend: Was rechtfertigt die vorliegende Arbeit?
Tatsächlich dürfte den wissenschaftsgeschichtlichen und -systematischen Erkenntnissen über Schnitzlers Psychologie - immer verstanden als Schnitzlers theoretische Position zu psychologischen Fragestellungen und nicht als die psychische Disposition des Autors - nach den Arbeiten von Michael Worbs, Horst Thomé und Dirk von Boetticher kaum noch etwas vollkommen Neues hinzuzufügen sein. Schnitzlers Haltung zu Psychologie und Medizin, seine eigenen psychologischen Konzeptionen und vor allem sein Verhältnis zur Freudschen Psychoanalyse sind in der Forschung umfassend, man darf wohl sagen: erschöpfend kommentiert worden. Weiterhin von Interesse bleibt hingegen die Frage, wie sich Schnitzlers Haltung zur Psychologie in seinen literarischen Werken niederschlägt, welcher interpretatorische Gewinn also - allgemein poetologisch sowie bei der konkreten Textdeutung - aus Schnitzlers Psychologie gezogen werden kann.
Im vorliegenden Beitrag soll gezeigt werden, dass sich das Konzept des "Narzissmus" für die Charakterisierung von Schnitzlers Psychologie in besonderer Weise eignet.
Flightless-I governs cell fate by recruiting the SUMO isopeptidase SENP3 to distinct HOX genes
(2017)
Background: Despite recent studies on the role of ubiquitin-related SUMO modifier in cell fate decisions, our understanding on precise molecular mechanisms of these processes is limited. Previously, we established that the SUMO isopeptidase SENP3 regulates chromatin assembly of the MLL1/2 histone methyltransferase complex at distinct HOX genes, including the osteogenic master regulator DLX3. A comprehensive mechanism that regulates SENP3 transcriptional function was not understood.
Results: Here, we identified flightless-I homolog (FLII), a member of the gelsolin family of actin-remodeling proteins, as a novel regulator of SENP3. We demonstrate that FLII is associated with SENP3 and the MLL1/2 complex. We further show that FLII determines SENP3 recruitment and MLL1/2 complex assembly on the DLX3 gene. Consequently, FLII is indispensible for H3K4 methylation and proper loading of active RNA polymerase II at this gene locus. Most importantly, FLII-mediated SENP3 regulation governs osteogenic differentiation of human mesenchymal stem cells.
Conclusion: Altogether, these data reveal a crucial functional interconnection of FLII with the sumoylation machinery that converges on epigenetic regulation and cell fate determination.
The p53 family of transcription factors (p53, p63 and p73) covers a wide range of functions critical for development, homeostasis and health of mammals across their lifespan. Beside the well-established tumor suppressor role, recent evidence has highlighted novel non-oncogenic functions exerted by p73. In particular, p73 is required for multiciliated cell (MCC) differentiation; MCCs have critical roles in brain and airways to move fluids across epithelial surfaces and to transport germ cells in the reproductive tract. This novel function of p73 provides a unifying cellular mechanism for the disparate inflammatory and immunological phenotypes of p73-deficient mice. Indeed, mice with Trp73 deficiency suffer from hydrocephalus, sterility and chronic respiratory tract infections due to profound defects in ciliogenesis and complete loss of mucociliary clearance since MCCs are essential for cleaning airways from inhaled pollutants, pathogens and allergens. Cross-species genomic analyses and functional rescue experiments identify TAp73 as the master transcriptional integrator of ciliogenesis, upstream of previously known central nodes. In addition, TAp73 shows a significant ability to regulate cellular metabolism and energy production through direct transcriptional regulation of several metabolic enzymes, such as glutaminase-2 and glucose-6 phosphate dehydrogenase. This recently uncovered role of TAp73 in the regulation of cellular metabolism strongly affects oxidative balance, thus potentially influencing all the biological aspects associated with p73 function, including development, homeostasis and cancer. Although through different mechanisms, p63 isoforms also contribute to regulation of cellular metabolism, thus indicating a common route used by all family members to control cell fate. At the structural level, the complexity of p73's function is further enhanced by its ability to form heterotetramers with some p63 isoforms, thus indicating the existence of an intrafamily crosstalk that determines the global outcome of p53 family function. In this review, we have tried to summarize all the recent evidence that have emerged on the novel non-oncogenic roles of p73, in an attempt to provide a unified view of the complex function of this gene within its family.
Wie keine andere kulturelle Handlung spiegelt das Tätowieren eine intentionale Ästhetisierung des menschlichen Körpers, zwischen individueller (und damit hoch-persönlicher) Schönheitsvorstellung auf der einen und öffentlicher (Selbst-)Inszenierung auf der anderen Seite schwankend. Die Haut wird dabei – quasi lebenslang beschrieben – zum Medium der Erinnerung. Zwar wurde bereits in der Antike 'tätowiert', der heutige Begriff sowie die 'Wiederentdeckung' dieser Praxis geht allerdings auf die Forschungs- und Entdeckungsreisen des 18. Jahrhunderts zurück, in denen Europäer mit fremden Kulturen in Kontakt kamen, die solche Verfahren mit einem ästhetischen oder rituellen Hintergrund praktizierten. So übernahm James Cook in seinen Aufzeichnungen den samoanischen Begriff "tatau", etymologisch die Grundlage für "to tattoo" und das deutsche "tätowieren". Inzwischen gilt die Modifikation des eigenen Körpers durch eine Tätowierung längst nicht mehr als 'verrucht' und stellt ebenso auch keine Ausnahmeerscheinung mehr dar, was sich vor allem an der explosionsartig gestiegenen Zahl von Tattoo-Studios in der Bundesrepublik ablesen lässt – und so trägt in Deutschland heute (konservativ geschätzt) etwa zehn Prozent der Gesamtbevölkerung und 23 Prozent der 16- bis 29-Jährigen mindestens ein Tattoo auf der Haut. Aber längst nicht jede Tätowierung ist auch der Ausdruck des eigenen Schönheitsempfindens oder eine vom Träger intentional auf dem Körper eingeschriebene Botschaft. Denn bereits in der griechischen Antike wurde das Verfahren auch dazu benutzt, Sklaven zu markieren, Besitzverhältnisse und damit ihre Unfreiheit auf der Haut einzuritzen; diese entpersonalisierende Markierung setzt sich mit der Häftlingstätowierung in Gefängnissen und schließlich den nationalsozialistischen Konzentrationslagern fort, die vor allem in literarischen Texten des Shoah-Diskures aufgegriffen und verhandelt wird.
The contributions of Korean and Taiwanese authors to the many and varied formulations of interwar pan-Asianism have so far remained a relatively unexplored subject of scholarly research, despite an unbroken interest in the trajectory of state-based Japanese pan-Asianism. Focusing on Korean students and independence activists, this article discusses alternative configurations of regional unity and solidarity that emanated from the interactions among Korean, Taiwanese, and other Asian actors who resided in Tokyo during the 1910s and 1920s. When the ethnic-nationalist interpretations of the Wilsonian principle of self-determination failed to materialize, a portion of anti-colonial activists in Asia began to emphasize the need for solidarity by drawing on what they perceived as traditional and shared “Asian” values. While challenging the Western-dominated international order of nation-states that perpetuated imperialism, such notions of Asian solidarity at the same time served as an ideology of liberation from Japanese imperialism. Examining journals published by Korean students and activists, including The Asia Kunglun, this article adds another layer to the history of pan-Asianism from below, a perspective that has often been neglected within the larger context of scholarship on pan-Asianism and Japanese imperialism in Asia.
Anxiety and politics
(2017)
The English version of this article was first published in 1957. The journal tripleC: Communication, Capitalism & Critique republished it 60 years later in 2017. In this essay, Franz L. Neumann discusses the role of anxiety in politics. The article asks: How does it happen that the masses sell their souls to leaders and follow them blindly? On what does the power of attraction of leaders over masses rest? What are the historical situations in which this identification of leader and masses is successful, and what view of history do the men have who accept leaders? For answering these questions, the author suggests a combination of political economy, Freudian political psychology, and ideology critique. He sees anxiety in the context of alienation. Alienation is analysed as a multidimensional phenomenon consisting of economic, political, social and psychological alienation. Neumann introduces the notions of Caesaristic identification, institutionalised anxiety and persecutory anxiety. The essay shows that fascism remains an actual threat in capitalist societies.
Autophagy acts through TRAF3 and RELB to regulate gene expression via antagonism of SMAD proteins
(2017)
Macroautophagy can regulate cell signalling and tumorigenesis via elusive molecular mechanisms. We establish a RAS mutant cancer cell model where the autophagy gene ATG5 is dispensable in A549 cells in vitro, yet promotes tumorigenesis in mice. ATG5 represses transcriptional activation by the TGFβ-SMAD gene regulatory pathway. However, autophagy does not terminate cytosolic signal transduction by TGFβ. Instead, we use proteomics to identify selective degradation of the signalling scaffold TRAF3. TRAF3 autophagy is driven by RAS and results in activation of the NF-κB family member RELB. We show that RELB represses TGFβ target promoters independently of DNA binding at NF-κB recognition sequences, instead binding with SMAD family member(s) at SMAD-response elements. Thus, autophagy antagonises TGFβ gene expression. Finally, autophagy-deficient A549 cells regain tumorigenicity upon SMAD4 knockdown. Thus, at least in this setting, a physiologic function for autophagic regulation of gene expression is tumour growth.
Ebola virus (EBOV) infection causes a high death toll, killing a high proportion of EBOV-infected patients within 7 days. Comprehensive data on EBOV infection are fragmented, hampering efforts in developing therapeutics and vaccines against EBOV. Under this circumstance, mathematical models become valuable resources to explore potential controlling strategies. In this paper, we employed experimental data of EBOV-infected nonhuman primates (NHPs) to construct a mathematical framework for determining windows of opportunity for treatment and vaccination. Considering a prophylactic vaccine based on recombinant vesicular stomatitis virus expressing the EBOV glycoprotein (rVSV-EBOV), vaccination could be protective if a subject is vaccinated during a period from one week to four months before infection. For the case of a therapeutic vaccine based on monoclonal antibodies (mAbs), a single dose might resolve the invasive EBOV replication even if it was administrated as late as four days after infection. Our mathematical models can be used as building blocks for evaluating therapeutic and vaccine modalities as well as for evaluating public health intervention strategies in outbreaks. Future laborator experiments will help to validate and refine the estimates of the windows of opportunity proposed here.
Although often depicted as rigid structures, proteins are highly dynamic systems, whose motions are essential to their functions. Despite this, it is difficult to investigate protein dynamics due to the rapid timescale at which they sample their conformational space, leading most NMR-determined structures to represent only an averaged snapshot of the dynamic picture. While NMR relaxation measurements can help to determine local dynamics, it is difficult to detect translational or concerted motion, and only recently have significant advances been made to make it possible to acquire a more holistic representation of the dynamics and structural landscapes of proteins. Here, we briefly revisit our most recent progress in the theory and use of exact nuclear Overhauser enhancements (eNOEs) for the calculation of structural ensembles that describe their conformational space. New developments are primarily targeted at increasing the number and improving the quality of extracted eNOE distance restraints, such that the multi-state structure calculation can be applied to proteins of higher molecular weights. We then review the implications of the exact NOE to the protein dynamics and function of cyclophilin A and the WW domain of Pin1, and finally discuss our current research and future directions.
The DACH concept is meant to offer both german teachers and german learners a theoretical approach for introducing information about german culture and civilization during language classes. It is based on the idea of pluricentricity, meaning that german language is definded on a cultural level by all places where it is spoken, considering Germany, Austria and Switzerland as it main centers. In the following article various course books and exam training materials are beign analysed with the intent to depict the way this concept is transfered from theory into practice.
The Karl Schwarzschild Meeting 2017 (KSM2017) has been the third instalment of the conference dedicated to the great Frankfurter scientist, who derived the first black hole solution of Einstein's equations about 100 years ago.
The event has been a 5 day meeting in the field of black holes, AdS/CFT correspondence and gravitational physics. Like the two previous instalments, the conference continued to attract a stellar ensemble of participants from the world's most renowned institutions. The core of the meeting has been a series of invited talks from eminent experts (keynote speakers) as well as the presence of plenary research talks by students and junior speakers.
List of Conference photo and poster, Sponsors and funding acknowledgments, Committees and List of participants are available in this PDF.
The marketing materials of remote eye-trackers suggest that data quality is invariant to the position and orientation of the participant as long as the eyes of the participant are within the eye-tracker’s headbox, the area where tracking is possible. As such, remote eye-trackers are marketed as allowing the reliable recording of gaze from participant groups that cannot be restrained, such as infants, schoolchildren and patients with muscular or brain disorders. Practical experience and previous research, however, tells us that eye-tracking data quality, e.g. the accuracy of the recorded gaze position and the amount of data loss, deteriorates (compared to well-trained participants in chinrests) when the participant is unrestrained and assumes a non-optimal pose in front of the eye-tracker. How then can researchers working with unrestrained participants choose an eye-tracker? Here we investigated the performance of five popular remote eye-trackers from EyeTribe, SMI, SR Research, and Tobii in a series of tasks where participants took on non-optimal poses. We report that the tested systems varied in the amount of data loss and systematic offsets observed during our tasks. The EyeLink and EyeTribe in particular had large problems. Furthermore, the Tobii eye-trackers reported data for two eyes when only one eye was visible to the eye-tracker. This study provides practical insight into how popular remote eye-trackers perform when recording from unrestrained participants. It furthermore provides a testing method for evaluating whether a tracker is suitable for studying a certain target population, and that manufacturers can use during the development of new eye-trackers.
In subduction zones, sediments and hydrothermally altered oceanic crust, which together form part of the subducting slab, contribute to the chemical composition of lavas erupted at the surface to form volcanic arcs. Transport of this material from the slab to the overlying mantle wedge is thought to involve discreet melts and fluids that are released from various portions of the slab. We use a meta-analysis of geochemical data from eight globally representative arcs to show that melts and fluids from individual slab components cannot be responsible for the formation of arc lavas. Instead, the data are compatible with models that first invoke physical mixing of slab components and the mantle wedge, widely referred to as high-pressure mélange, before arc magmas are generated.
We study anisotropic fluid dynamics derived from the Boltzmann equation based on a particular choice for the anisotropic distribution function within a boost-invariant expansion of the fluid in one spatial dimension. In order to close the conservation equations we need to choose an additional moment of the Boltzmann equation. We discuss the influence of this choice of closure on the time evolution of fluid-dynamical variables and search for the best agreement to the solution of the Boltzmann equation in the relaxation-time approximation.
Reticulate evolution is considered to be among the main mechanisms of plant evolution, often leading to the establishment of new species. However, complex evolutionary scenarios result in a challenging definition of evolutionary and taxonomic units. In this study, we aimed to examine the evolutionary origin and revise the species status of Campanula baumgartenii, a rare endemic species from the polyploid complex Campanula section Heterophylla. Morphometry, flow cytometric ploidy estimation, amplified fragment length polymorphisms (AFLPs), as well as chloroplast and nuclear DNA sequence markers were used to assess the morphological and genetic differentiation among C. baumgartenii, Campanula rotundifolia and other closely related taxa. Tetra- and hexaploid C. baumgartenii is morphologically and molecularly (AFLP) differentiated from sympatric C. rotundifolia. Contrasting signals from nuclear (ITS) and chloroplast (trnL-rpl32) markers suggest a hybrid origin of C. baumgartenii with C. rotundifolia and a taxon related to the alpine Campanula scheuchzeri as ancestors. Additionally, hexaploid C. baumgartenii currently hybridizes with co-occurring tetraploid C. rotundifolia resulting in pentaploid hybrids, for which C. baumgartenii serves as both seed and pollen donor. Based on the molecular and morphological differentiation, we propose to keep C. baumgartenii as a separate species. This study exemplifies that detailed population genetic studies can provide a solid basis for taxonomic delimitation within Campanula section Heterophylla as well as for sound identification of conservation targets.
The iconic Australasian kangaroos and wallabies represent a successful marsupial radiation. However, the evolutionary relationship within the two genera, Macropus and Wallabia, is controversial: mitochondrial and nuclear genes, and morphological data have produced conflicting scenarios regarding the phylogenetic relationships, which in turn impact the classification and taxonomy. We sequenced and analyzed the genomes of 11 kangaroos to investigate the evolutionary cause of the observed phylogenetic conflict. A multilocus coalescent analysis using ∼14,900 genome fragments, each 10 kb long, significantly resolved the species relationships between and among the sister-genera Macropus and Wallabia. The phylogenomic approach reconstructed the swamp wallaby (Wallabia) as nested inside Macropus, making this genus paraphyletic. However, the phylogenomic analyses indicate multiple conflicting phylogenetic signals in the swamp wallaby genome. This is interpreted as at least one introgression event between the ancestor of the genus Wallabia and a now extinct ghost lineage outside the genus Macropus. Additional phylogenetic signals must therefore be caused by incomplete lineage sorting and/or introgression, but available statistical methods cannot convincingly disentangle the two processes. In addition, the relationships inside the Macropus subgenus M. (Notamacropus) represent a hard polytomy. Thus, the relationships between tammar, red-necked, agile, and parma wallabies remain unresolvable even with whole-genome data. Even if most methods resolve bifurcating trees from genomic data, hard polytomies, incomplete lineage sorting, and introgression complicate the interpretation of the phylogeny and thus taxonomy.
In this study, we investigate the interaction of jets with their environment at a microscopic level, which is a key open question in the study of relativistic jets. Using small simulation systems during past research, we initially studied the evolution of both electron–proton and electron–positron relativistic jets containing helical magnetic fields, by focusing on their interactions with an ambient plasma. Here, using larger jet radii, we have performed simulations of global jets containing helical magnetic fields in order to examine how helical magnetic fields affect kinetic instabilities, such as the Weibel instability, the kinetic Kelvin–Helmholtz instability (kKHI) and the mushroom instability (MI). We found that the evolution of global jets strongly depends on the size of the jet radius. For example, phase bunching of jet electrons, in particular in the electron–proton jet, is mixed with a larger jet radius as a result of the more complicated structures of magnetic fields with excited kinetic instabilities. In our simulation, these kinetic instabilities led to new types of instabilities in global jets. In the electron–proton jet simulation, a modified recollimation occurred, and jet electrons were strongly perturbed. In the electron–positron jet simulation, mixed kinetic instabilities occurred early, followed by a turbulence-like structure. Simulations using much larger (and longer) systems are required in order to further thoroughly investigate the evolution of global jets containing helical magnetic fields.
The present paper accumulates information and studies the etymology of the Romanian ethnonym “Aleman” and its versions, beginning from their geographical spread throughout Romania and Germany. The Romanian surnames “Aleman” and “Aloman” (highest prevalence in the area of Transylvania, in the Sibiu and Alba counties), as well as “Aliman”, “Alimănescu”, “Alaman” and “Alman” (highest prevalence in the areas of Muntenia, Oltenia and Dobrogea) do not come from the French term “allman” as their German equivalents “Allman”, “Allmang”, “Lallemand” do, which are concentrated in the Western Germany (in the Saarland, Rhineland-Palatinate German federal districts), due to the fact that there are no correspondents to the surnames of the Transylvanian Saxons. Therefore, the origin of these Romanian surnames is more likely to relate to the Turkish term “aleman” (see Iordan, 1983, p. 25 and 23), which also refers to the Germanic tribe of alamans or alemans, having the same meaning of “German”. The geographical proliferation of the “Aleman” and “Aliman” versions of the term is specific to the East to West population migration phenomena. These versions are the only ones existent in today’s Germany. Thus the “Aleman” and “Aliman” surnames are to be found in strongly industrialized centers such as Munich, Stuttgart, Karlsruhe, Mannheim, Wiesbaden, Bielefeld, Hamburg and not in the area of the German-French frontier (see following map).
Self-extracellular RNA (eRNA), released from stressed or injured cells upon various pathological situations such as ischemia-reperfusion-injury, has been shown to act as an alarmin by inducing procoagulatory and proinflammatory responses. In particular, M1-polarization of macrophages by eRNA resulted in the expression and release of a variety of cytokines, including tumor necrosis factor (TNF)-α or interleukin-6 (IL-6). The present study now investigates in which way self-eRNA may influence the response of macrophages towards various Toll-like receptor (TLR)-agonists. Isolated agonists of TLR2 (Pam2CSK4), TLR3 (PolyIC), TLR4 (LPS), or TLR7 (R848) induced the release of TNF-α in a concentration-dependent manner in murine macrophages, differentiated from bone marrow-derived stem cells by mouse colony stimulating factor. Here, the presence of eRNA shifted the dose-response curve for Pam2CSK4 (Pam) considerably to the left, indicating that eRNA synergistically enhanced the cytokine liberation from macrophages even at very low Pam-levels. The synergistic activation of TLR2 by eRNA/Pam was duplicated by other TLR2-agonists such as FSL-1 or Pam3CSK4. In contrast, for TLR4-agonists such as LPS a synergistic effect of eRNA was much weaker, and was not existent for TLR3-, or TLR7-agonists. The synergistic eRNA/Pam action was dependent on the NFκB-signaling pathway as well as on p38MAP- and MEK1/ERK-kinases and was prevented by predigestion of eRNA with RNase1 or by antibodies against TLR2. Thus, the presence of self-eRNA as alarming molecule sensitizes innate immune responses towards pathogen-associated molecular patterns (PAMPs) in a synergistic way and may thereby contribute to the differentiated outcome of inflammatory responses.
Online reading behavior can be regarded as a "new" form of cultural capital in today’s digital world. However, it is unclear whether "traditional" mechanisms of cultural and social reproduction are also found in this domain, and whether they manifest uniformly across countries at different stages of development. This article analyzes whether the early home literacy environment has an impact on informational online reading behavior among adolescents and whether this association varies between countries with different levels of digitalization and educational expansion. Data from the 2009 Programme for International Student Assessment (PISA) were used for the empirical analyses. The results of regression models with country-fixed effects indicate a positive association between literacy activities in early childhood and informational online reading at age 15. This association was quite stable across countries. These findings are discussed in light of cultural and social reproduction theory and digital divide research.
Transitional between the Pannonian Basin and the Eastern Carpathians the Transcarpathian Ukraine (Transcarpathia) has a diverse geology including Quaternary sediments, volcanites, limestones and flysch, and its climate at low altitudes is relatively warm and humid. We conducted a field survey in June 2016 focusing on mesophilous forest vegetation along a transect covering almost the whole lowaltitudinal part of Transcarpathia. We recorded 54 relevés in the field and further digitized 22 relevés from literature. Using classification analysis, we distinguished three clusters of oak-hornbeam forests (alliance Carpinion betuli; 1–3) and three clusters of scree and ravine forests (alliance Tilio platyphylli-Acerion; 4–6): (1) Slightly wet Pannonian oak-hornbeam forests occurring in the lowland part of the region (Circaeo-Carpinetum); (2) Mesophilous oak-hornbeam forests (Carici pilosae-Carpinetum); (3) Xero-mesophilous oak-hornbeam forests (a drier subtype of the Carici pilosae-Carpinetum and the association Primulo veris-Carpinetum); (4) Mesophilous scree forests (Phyllitido-Aceretum); (5) Forests of steep slopes (Aceri-Tilietum) and transitions to mesophilous oak-hornbeam forests (Carici pilosae-Carpinetum); (6) Cool and wet scree and ravine forests (Arunco dioici-Aceretum pseudoplatani). Using indirect ordination analysis, three environmental variables (altitude, heat load index and slope) were identified as factors of significant influence on the species composition. These factors well distinguish oak-hornbeam forests from scree and ravine forests.
Vom 16. bis 18. März 2017 fand am Institut für Germanistik der Universität Wroclaw eine internationale sprachwissenschaftliche Konferenz statt. Die Tagung mit dem Rahmenthema 'Intra- und interlinguale Zugänge zur deutschen Phraseologie und Parömiologie' wurde vom Lehrstuhl für Angewandte Linguistik des Instituts für Germanistik der Universität Wroclaw in Zusammenarbeit mit dem Institut für Slawistik (FR Westslawistik) der Universität Leipzig veranstaltet.
Bu makalede geleneksel Alman genç kız romanının temel eserlerinden birisi olan Emmy von Rhoden'in "Der Trotzkopf" (1885) adlı romanı ile modern dönemi temsilen Dagmar Chidolue'nun "Lady Punk" (1985) adlı romanı toplumsal cinsiyet araştırmaları ile yeniden okunmuştur. Bu okuma ve analiz süreci sonucunda kurgusal genç kız imgesinin gelenekselden moderne doğru geçirdiği değişim karşılaştırmalı olarak ortaya konmaya çalışılmıştır. Diğer bir hedef ise modern döneme gelindiğinde genç kız imgesinin lehine olan özgürleşmesinde rol oynayan etmenleri ve kazandığı özgürlüğün niteliklerini vurgulamaktır. Sonuçların toplumsal cinsiyet araştırmalarına dayanılarak yorumlanması sürecinde ise çalışmaya her iki romandan elde edilen bulgular rehberlik etmiştir.
Paricalcitol is approved for prevention and therapy of secondary hyperparathyroidism (sHPT) in patients with chronic kidney disease (CKD), with only short-term data in clinical routine settings. A 12-month observational study was conducted in Germany and Austria (90 centers, 761 patients) from 2008 to 2013. Laboratory values, demographical, and clinical data were documented in 629 dialysis patients and 119 predialysis patients. In predialysis patients, median intact parathormone (iPTH) was 180.0 pg/mL (n = 105) at the start of the study, 115.7 pg/mL (n = 105) at last documentation, and 151.8 pg/mL (n = 50) at month 12, with 32.4% of the last documented iPTH values in the KDOQI (Kidney Disease Outcomes Quality Initiative) target range. In dialysis patients, median iPTH was 425.5 pg/mL (n = 569) at study start, 262.3 pg/mL (n = 569) at last documentation, and 266.1 pg/mL (n = 318) at month 12, with 36.5% of dialysis patients in the KDOQI target range. Intravenous paricalcitol showed more homogenous iPTH control than oral treatment. Combined analysis of all dialysis patients indicated comparable and stable mean serum calcium and phosphate levels throughout the study. Clinical symptoms, such as itching, bone pain, and fatigue, were improved compared with study entry. The spectrum and frequency of adverse events mirrored the known pattern for patients on dialysis. Paricalcitol is efficacious and has a consistent safety profile in sHPT over 12 months.
This article reports the results of a replication of Bobbitt-Zeher’s 2007 article "The Gender Income Gap and the Role of Education". Models that emulate the original specifications (by and large) reproduce the original results. However, models that adhere to Bobbitt-Zeher’s theory concerning the gendered effect of family formation call into question her finding that "values appear to matter only modestly, while family formation has virtually no effect on the income gap".
Als methodisches Rüstzeug bieten sich im Falle des hier verfolgten Themas vier Zugänge an: Erstens eine ideengeschichtliche Rekonstruktion der jüdischen Neuromantik, gegliedert nach verschiedenen Phasen (Vorgeschichte, zionistische Auslegung, ästhetische Kontroversen). Zweitens ein kulturwissenschaftlich ausgerichteter Konstellationsforschungsansatz. [...] Drittens bietet sich das Thema jüdische Neuromantik geradezu dafür an, die jüngst im englisch-amerikanischen Theoriefeld wieder aufgegriffenen methodischen Überlegungen zu kulturwissenschaftlich ausgerichteten "translation studies" einzubeziehen; [...] Und schließlich erscheint eine begriffliche Differenzierung nützlich. Im Blick auf die kulturpolitisch ausgerichtete zionistische Bewegung dürfte es angemessen sein, von einer "Wiederaufnahme" romantischer Vorgaben aus der Zeit um 1800 zu sprechen. Der zeitgenössische kulturpoetisch-ästhetische Versuch einer genaueren Charakterisierung neuromantischer Gattungen und Schreibweisen dürfte allerdings treffsicherer mit dem Begriff "gesteigerte Wiederkehr" umschrieben werden können.
Kinematic analysis of work-related musculoskeletal loading of trunk among dentists in Germany
(2017)
BACKGROUND: In Germany, about 86.7 % of the dentists have stated to suffer from pain in the neck and shoulder region. These findings are predominantly based on surveys. Therefore the objective of this study is to conduct a kinematic analysis of occupational posture in dentistry.
METHODS: Twenty one dentists (11 f/10 m; age: 40.1 ± 10.4 years) have participated in this examination. The CUELA-System was used to collect kinematic data of the activities on an average dental workday. A detailed, computer-based task analysis took place parallel to the kinematic examination. Through the synchronization of data collected from both measurements, patterns of posture were arranged chronologically and in conjunction with the tasks performed: (I) "treatment" (II) "office" and (III) "other activities". For the data analysis, characteristic data of joint angular distributions (percentiles P05, P25, P50, P75 and P95) of head, neck and torso at pre-defined tasks were examined and assessed corresponding to ergonomic standards.
RESULTS: Forty one percent of tasks executed on an average dental workday can be categorized as the treatment of patients. These tasked are most frequently performed in "straight back" positions (78.7 %), whereas 20.1 % were carried out in a "twisted or inclined" torso posture, 1.1 % "bowed" and only 0.1 % "bowed and twisted/inclined to the side" upper body position. In particular, it can be observed that in the area of the cervical and thoracic spine the 75th and 95th percentile show worse angular values during treatment than during non-dental tasks. For the period of treatment (at a standardized dental chair construction), a seated position with a strong inclination of the thoracic spine to the right while the lumbar spine is inclined towards the left is adopted.
CONCLUSION: The kinematic analysis of dentists illustrates typical patterns of postures during tasks that are essential to the dental treatment of patients. The postures in the area of the cervical and thoracic spine have higher angular values during treatment compared to other dental tasks. Consistently, appropriate ergonomic design measures to optimize the dental chair and equipment as well as integrated training in ergonomics as part of the study of dentistry to prevent musculoskeletal are recommended.
Background: This study aims at identifying orthodontic activities with the highest frequency of unfavorable/awkward and static postures held over a period of more than 4 s based on kinematic analysis. Moreover, a separate analysis of static postures for orthodontic and non-orthodontic activities serves to evaluate the duration for which these particular postures are assumed.
Methods: In total, 21 (13f/8 m) orthodontists (age: 31.5 ± 3.8 years) participated in this study. CUELA, a personal measurement system, was used to collect kinematic data for all orthodontic activities in a working day. Angle values of the head and torso were evaluated in accordance with ergonomic standards. Only those postures that were held statically for 4 s and longer were selected for further analysis. Alongside the kinematic analysis, the activities performed on-site were also subject to a detailed computerized analysis. The synchronization of data collected from both measurements arranges the patterns of posture found chronologically and in conjunction with the orthodontic activities performed ((I) "treatment" (II) "office" and (III) "other activities").
Results: For (I) we observed an anterior inclination of the head and torso area as well as a twist of the head and neck area to the right. We found anterior back inclination and lateral back torsion to the right for (II) and (III). If, furthermore, we differentiate the duration of static postures, there are primarily short to medium-term (4–30s) static postures identified for (I). Also, categories (II) and (III) predominantly demonstrate static back postures with a duration of up to 30 s. With regard to (II) we observed that the back is ventrally inclined for 10.1% of the total activity duration.
Conclusions: During treatment static strains are observed in the entire head and torso area. On the contrary, static postures prevalent in the torso area are essential for activities of the other categories, particularly office work. These findings allow for a careful selection of unfavorable and static postures for each of the activities performed and help to develop specific preventive measures.
Background: How a dentist works, such as the patterns of movements performed daily, is also largely affected by the workstation Dental tasks are often executed in awkward body positions, thereby causing a very high degree of strain on the corresponding muscles. The objective of this study is to detect those dental tasks, during which awkward postures occur most frequently. The isolated analysis of static postures will examine the duration for which these postures are maintained during the corresponding dental, respectively non-dental, activities.
Methods: 21 (11f/10 m) dentists (age: 40.1 ± 10.4 years) participated in this study. An average dental workday was collected for every subject. To collect kinematic data of all activities, the CUELA system was used. Parallel to the kinematic examination, a detailed computer-based task analysis was conducted. Afterwards, both data sets were synchronized based on the chronological order of the postures assumed in the trunk and the head region. All tasks performed were assigned to the categories "treatment" (I), "office" (II) and "other activities" (III). The angle values of each body region (evaluation parameter) were examined and assessed corresponding to ergonomic standards. Moreover, this study placed a particular focus on static positions, which are held statically for 4 s and longer.
Results: For "treatment" (I), the entire head and trunk area is anteriorly tilted while the back is twisted to the right, in (II) and (III) the back is anteriorly tilted and twisted to the right (non-neutral position). Static positions in (I) last for 4–10s, static postures (approx. 60%) can be observed while in (II) and (III) in the back area static positions for more than 30 s are most common. Moreover, in (II) the back is twisted to the right for more than 60 s in 26.8%.
Conclusion: Awkward positions are a major part of a dentists’ work. This mainly pertains to static positions of the trunk and head in contrast to "office work." These insights facilitate the quantitative description of the dentist profession with regard to the related physical load along with the health hazards to the musculoskeletal system. Moreover, the results allow for a selective extraction of the most unfavorable static body positions that dentists assume for each of the activities performed.
Objectives The following study analyses the influence of risk factors among the occupational group of truck drivers on postural control and body mass index (BMI).
Design Observational study.
Setting One motorway station close to several highways in Germany.
Participants 180 truck drivers (177 male/3 female), aged 21–65 years old, took part in this study.
Outcome measures Postural control was examined using a pressure plate. In order to examine the influence of body weight (BMI) and working years on postural control, subjects were divided into samples of five and three groups, respectively. Furthermore, it was evaluated whether the subjects suffered from back pain. For data analysis, the Kruskal-Wallis test was used as the data were not normally distributed. Once the p value of the Kruskal-Wallis test was p≤0.05, the Conover-Iman comparison and afterwards the Bonferroni-Holm correction were used. The significance level was set at α ≤0.05.
Results Regarding the number of working years, a significant increase of frontal (p≤0.04) and sagittal (p≤0.001) sway were observed. The correlation of the five BMI groups with the number of working years demonstrates that an increase of the working years leads to an increase of BMI (p≤0.03). Furthermore, the majority of truck drivers participating in this study suffered from back pain (61.7%).
Conclusions BMI and musculoskeletal impairment are indicators of health risk factors. In this study, it is shown that an increasing number of working years and an increasing BMI lead to a decrease in frontal and sagittal postural sway. In addition, the number of working years correlates with body weight and back pain.
Fit to play : posture and seating position analysis with professional musicians - a study protocol
(2017)
Background: Musical performance-associated musculoskeletal disorders (MSD) are a common health problem among professional musicians. Considering the manifold consequences arising for the musicians, they can be seen as a threat for their professional activity. String players are the most affected group of musicians in this matter. Faults in upper body posture while playing the instrument, causing un-ergonomic static strain on the back and unergonomic limp-movements, are a main reason for musculoskeletal disorders and pain syndromes.
Methods: A total of 66 professional musicians, divided into three groups, are measured.
The division is performed by average duration of performance, intensity of daily exercise and professional experience. Video raster stereography, a three-dimensional analysis of the body posture, is used to analyse the instrument-specific posture. Furthermore the pressure distribution during seating is analysed. Measurements are performed because the musician is sitting on varying music chairs differing in structure and/or construction of the seating surface. The measurements take place in habitual seating position as well as during playing the instrument.
Results: To analyse the influence of different chairs, ANOVA for repeated measurements or Friedman-test is used, depending on normality assumptions. Comparison of posture between amateur musicians, students, and professional orchestral musicians is carried out the non-parametric Jonckheere-Terpstra-test.
Conclusions: Our method attempts to give the musicians indications for the right music chair choice by analyzing the chair concepts, so that thereby preemptively MSD can be reduced or prevented.
Während der Streit um den Verfassungsgerichtshof aus polnischer Sicht als mittlerweile beendet gilt, versucht die Europäische Union eine passende Lösung zu finden, die in der Empfehlung der Kommission geäußerten Forderungen durchzusetzen. Die regierende Partei "Recht und Gerechtigkeit" beschließt jedoch in der Zwischenzeit weitere Justizreformen, die genauso, wie das umstrittene Verfassungsgerichtshofgesetz, gegen die Grundsätze der Europäischen Union aus Art. 2 EUV, vor allem gegen die Rechtsstaatlichkeit und Demokratie, verstoßen können. ...
Der vorliegende Beitrag beschäftigt sich mit der Präsenz der Gewaltproblematik im Zusammenhang mit der Verbreitung der Literatur von unerwünschten DDR-Autoren in der VR Polen. Die Gründung einer alternativen polnischen Kulturszene Mitte der 1970er Jahre konnte teilweise eine Lücke schließen, die das in beiden Ländern verhängte Einreiseverbot für diese Schriftsteller und ihre Texte hinterlassen hatte. Da solche Aktivitäten als eine Straftat mit aller Schärfe verfolgt wurden, waren verschiedene Formen der Gewaltanwendung ein Bestandteil der staatlichen Überwachung und Einschüchterung von Schriftstellern, Verlegern, Übersetzern und Publizisten.
The sphingolipid sphingosine-1-phosphate (S1P) emerges as an important regulator of immunity, mainly by signaling through a family of five specific G protein-coupled receptors (S1PR1–5). While S1P signaling generally has the potential to affect not only trafficking but also differentiation, activation, and survival of a diverse range of immune cells, the specific outcome depends on the S1P receptor repertoire expressed on a given cell. Among the S1PRs, S1PR4 is specifically abundant in immune cells, suggesting a major role of the S1P/S1PR4 axis in immunity. Recent studies indeed highlight its role in activation of immune cells, differentiation, and, potentially, trafficking. In this review, we summarize the emerging data that support a major role of S1PR4 in modulating immunity in humans and mice and discuss therapeutic implications.
Mutations are the ultimate basis of evolution, yet their occurrence rate is known only for few species. We directly estimated the spontaneous mutation rate and the mutational spectrum in the nonbiting midge C. riparius with a new approach. Individuals from ten mutation accumulation lines over five generations were deep genome sequenced to count de novo mutations that were not present in a pool of F1 individuals, representing parental genotypes. We identified 51 new single site mutations of which 25 were insertions or deletions and 26 single nucleotide mutations. This shift in the mutational spectrum compared to other organisms was explained by the high A/T content of the species. We estimated a haploid mutation rate of 2.1 × 10−9 (95% confidence interval: 1.4 × 10−9 – 3.1 × 10-9) that is in the range of recent estimates for other insects and supports the drift barrier hypothesis. We show that accurate mutation rate estimation from a high number of observed mutations is feasible with moderate effort even for nonmodel species.
Cellular attachment plays a vital role in the differentiation of pheochromocytoma (PC12) cells. PC12 cells are noradrenergic clonal cells isolated from the adrenal medulla of Rattus norvegicus and studied extensively as they have the ability to differentiate into sympathetic neuron-like cells. The effect of several experimental parameters including (i) the concentration of nerve growth factor (NGF); (ii) substratum coatings, such as poly-L-lysine (PLL), fibronectin (Fn), and laminin (Lam); and (iii) double coatings composed of PLL/Lam and PLL/Fn on the differentiation process of PC12 cells were studied. Cell morphology was visualised using brightfield phase contrast microscopy, cellular metabolism and proliferation were quantified using a 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay, and the neurite outgrowth and axonal generation of the PC12 cells were evaluated using wide field fluorescence microscopy. It was found that double coatings of PLL/Lam and PLL/Fn supported robust adhesion and a two-fold enhanced neurite outgrowth of PC12 cells when treated with 100 ng/mL of NGF while exhibiting stable metabolic activity, leading to the accelerated generation of axons.
So unterschiedlich die betrachteten Texte in ihren Formen und Themen auch sind, sie alle zeigen, dass der überkommenen Sprache ein Normensystem inhärent ist, das überwunden bzw. umgestürzt werden muss, um die politischen Veränderungen und die Anwendung von Gewalt zu legitimieren und zu ermöglichen. Weder Orwell, Klemperer noch Thukydides schlagen Remedia oder Prophylaxen gegen die Manipulation der Sprache vor. Sie finden sich jedoch implizit in den entsprechenden Texten und manifestieren sich explizit durch die Existenz der Werke selbst: Das Bollwerk gegen eine instrumentelle Veränderung der Sprache ist das Gedächtnis, sei es das individuelle, sei es das kollektiv-kulturelle. Wir können zwar sicher sein, dass zumindest Thukydides die Idee eines Gewissens der Wörter äußerst fremd ist. Doch mit einem Konzept, ohne das es das Gewissen nicht geben kann, ist er zweifelsohne vertraut: dem der Erinnerung. Die Erinnerung kann einen davor bewahren, Propheten welcher Art auch immer zu folgen, überlieferte Bedeutungen zu vergessen und dem Denken zu entsagen. Mnemosyne heißt die Erinnerung in der griechischen Mythologie. Sie hat neun Töchter. Eine davon ist Klio, die Muse der Geschichtsschreibung.
The fractional release factor (FRF) gives information on the amount of a halocarbon that is released at some point into the stratosphere from its source form to the inorganic form, which can harm the ozone layer through catalytic reactions. The quantity is of major importance because it directly affects the calculation of the ozone depletion potential (ODP). In this context time-independent values are needed which, in particular, should be independent of the trends in the tropospheric mixing ratios (tropospheric trends) of the respective halogenated trace gases. For a given atmospheric situation, such FRF values would represent a molecular property.
We analysed the temporal evolution of FRF from ECHAM/MESSy Atmospheric Chemistry (EMAC) model simulations for several halocarbons and nitrous oxide between 1965 and 2011 on different mean age levels and found that the widely used formulation of FRF yields highly time-dependent values. We show that this is caused by the way that the tropospheric trend is handled in the widely used calculation method of FRF.
Taking into account chemical loss in the calculation of stratospheric mixing ratios reduces the time dependence in FRFs. Therefore we implemented a loss term in the formulation of the FRF and applied the parameterization of a mean arrival time to our data set.
We find that the time dependence in the FRF can almost be compensated for by applying a new trend correction in the calculation of the FRF. We suggest that this new method should be used to calculate time-independent FRFs, which can then be used e.g. for the calculation of ODP.
Background. Arterial ex situ back-table perfusion (BP) reportedly reduces ischemic-type biliary lesion after liver transplantation. We aimed to verify these findings in a prospective investigation.
Methods. Our prospective, randomized, controlled, multicenter study involved livers retrieved from patients in 2 German regions, and compared the outcomes of standard aortic perfusion to those of aortic perfusion combined with arterial ex situ BP. The primary endpoint was the incidence of ischemic-type biliary lesions over a follow-up of 2 years after liver transplantation, whereas secondary endpoints included 2-year graft survival, initial graft damage as reflected by transaminase levels, and functional biliary parameters at 6 months after transplantation.
Results. A total of 75 livers preserved via standard aortic perfusion and 75 preserved via standard aortic perfusion plus arterial BP were treated using a standardized protocol. The incidence of clinically apparent biliary lesions after liver transplantation (n = 9 for both groups; P = 0.947), the 2-year graft survival rate (standard aortic perfusion, 74%; standard aortic perfusion plus arterial BP, 68%; P = 0.34), and incidence of initial graft injury did not differ between the 2 perfusion modes. Although 33 of the 77 patients with cholangiography workups exhibited injured bile ducts, only 10 had clinical symptoms.
Conclusions. Contrary to previous findings, the present study indicated that additional ex situ BP did not prevent ischemic-type biliary lesions or ischemia-reperfusion injury after liver transplantation. Moreover, there was considerable discrepancy between cholangiography findings regarding bile duct changes and clinically apparent cholangiopathy after transplantation, which should be considered when assessing ischemic-type biliary lesions.
A considerable body of the literature considers the potential impact of exotic predators on native prey organisms, while comparatively, few studies have asked whether and how native predators include novel prey types into their diet spectrum. Here, we asked whether the native aquatic heteropteran Diplonychus esakii preys on the highly invasive western mosquitofish (Gambusia affinis), which has been introduced to southern China and threatens native fish species through competition and predation on their fry. We conducted 48-h prey choice experiments under semi-natural conditions. In a ‘no-choice’ experiment (one predator and one potential prey; n = 200), we found the heteropterans to prey more on large-bodied fish, a pattern that was also described for other belostomatids, while prey sex had no effect on capture rates. Moreover, large-bodied heteropterans caught more fish than small-bodied individuals. However, overall capture rates in our study were low (11.5–30%) compared to studies on other belostomatids, which explains why subsequent binary prey choice experiments using one predator and two prey—either large and small females or male and female (with smaller sample sizes of n = 20 and 30, respectively)—did not confirm the results of our first experiment. Our study exemplifies how a pattern of body size-dependent predation can arise in a novel (not coevolved) predator–prey interaction. We tentatively argue that the observed pattern could be driven by intrinsic features of the predator, namely, altered prey preferences with increasing age coupled with a general preference for large-bodied prey, or changing nutritional needs at different developmental stages.
Die vorliegende Untersuchung beabsichtigt die Schönheitsauffassung in der deutschen und türkischen Erzählwelt in ihrer Bedeutung, Funktionalität und kulturellen Einbettung herauszuarbeiten. Ausgehend von den Märchen der Brüder Grimm (Erstausgabe von 1812 und 1815) und den türkischen Märchen von Ignácz Kúnos (Erstfassung,1887) werden die Begriffe "schön", "Schönheit" und "Ästhetik" analysiert und zur Diskussion gestellt. Mittels einer Korpusanalyse werden so die Märchentexte einer hermeneutisch-analytischen, mythologischen und kulturtheoretisch-kritischen Betrachtung unterzogen. Ziel dabei sind die Maßstäbe der Schönheit zu hinterfragen. Was wird in der Märchenwelt als "schön" empfunden? Worin liegt die Grenze vom Schönen zum Hässlichen? Hier ist auch zu fragen, inwieweit das kulturelle Umfeld die Auffassung zum Schönen beeinträchtigt
Managing for biodiversity: impact and action thresholds for invasive plants in natural ecosystems
(2017)
Some studies examining the impacts of invasive plant species in native plant communities have demonstrated non-linear damage functions, whereby community components, such as species richness, are seemingly unaffected by the presence of an invader until it has attained relatively high levels of abundance, whereupon there is a marked decrease with further increases in abundance. Given chronic limitations in the resources available for managing invasive species, it has been argued that the most damaging invaders would be controlled most efficiently by maintaining their abundances below such threshold levels. Because many impact studies do not involve sampling over a wide range of invader abundances it is not possible to estimate the prevalence of threshold relationships. Furthermore, studies that have employed appropriate sampling methods have shown that different life forms exhibit different threshold responses, indicating that maintenance management for biodiversity values should be designed to protect the most sensitive species or groups of species. Since control costs increase with invader abundance, economic and ecological considerations are aligned when invaders are sustainably maintained at relatively low abundances. Adopting such an approach should also minimise negative impacts where damage functions are linear.