Edna Bode, Antje Katharina Heinrich, Merle Hirschmann, Desalegne Abebew, Yan-Ni Shi, Tien Duy Vo, Frank Wesche, Yi-Ming Shi, Peter Grün, Svenja Simonyi, Nadine Keller, Yvonne Engel, Sebastian Wenski, Reuel Bennet, Sophie Beyer, Iris Bischoff, Anthony Tumenez Buaya, Sophie Brandt, Ibrahim Cakmak, Harun Çimen, Simone Eckstein, Denia Frank, Robert Fürst, Martin Gand, Gerd Geisslinger, Selcuk Hazir, Marina Henke, Ralf Heermann, Virginie Lecaudey, Wilhelm Schäfer, Susanne Schiffmann, Anja Schüffler, Rebecca Schwenk, Marisa Skaljac, Eckhard Thines, Marco Thines, Thomas Ulshöfer, Andreas Vilcinskas, Thomas Alexander Wichelhaus, Helge Björn Bode
- Natural products (NPs) from microorganisms have been important sources for discovering new therapeutic and chemical entities. While their corresponding biosynthetic gene clusters (BGCs) can be easily identified by gene-sequence-similarity-based bioinformatics strategies, the actual access to these NPs for structure elucidation and bioactivity testing remains difficult. Deletion of the gene encoding the RNA chaperone, Hfq, results in strains losing the production of most NPs. By exchanging the native promoter of a desired BGC against an inducible promoter in Δhfq mutants, almost exclusive production of the corresponding NP from the targeted BGC in Photorhabdus, Xenorhabdus and Pseudomonas was observed including the production of several new NPs derived from previously uncharacterized non-ribosomal peptide synthetases (NRPS). This easyPACId approach (easy Promoter Activated Compound Identification) facilitates NP identification due to low interference from other NPs. Moreover, it allows direct bioactivity testing of supernatants containing secreted NPs, without laborious purification.
MetadatenAuthor: | Edna BodeORCiDGND, Antje Katharina Heinrich, Merle HirschmannGND, Desalegne AbebewORCiD, Yan-Ni Shi, Tien Duy Vo, Frank WescheORCiD, Yi-Ming ShiORCiDGND, Peter Grün, Svenja Simonyi, Nadine Keller, Yvonne Engel, Sebastian Wenski, Reuel Bennet, Sophie Beyer, Iris BischoffORCiDGND, Anthony Tumenez BuayaGND, Sophie BrandtORCiD, Ibrahim CakmakORCiD, Harun ÇimenORCiD, Simone Eckstein, Denia Frank, Robert FürstORCiDGND, Martin GandORCiD, Gerd GeisslingerORCiDGND, Selcuk HazirORCiD, Marina HenkeORCiD, Ralf HeermannORCiD, Virginie LecaudeyORCiDGND, Wilhelm Schäfer, Susanne SchiffmannORCiDGND, Anja SchüfflerORCiD, Rebecca Schwenk, Marisa SkaljacORCiD, Eckhard ThinesORCiDGND, Marco ThinesORCiDGND, Thomas Ulshöfer, Andreas VilcinskasORCiDGND, Thomas Alexander WichelhausORCiD, Helge Björn BodeORCiDGND |
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URN: | urn:nbn:de:hebis:30:3-638202 |
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DOI: | https://doi.org/10.1002/anie.201910563 |
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ISSN: | 1521-3773 |
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Parent Title (English): | Angewandte Chemie |
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Publisher: | Wiley-VCH |
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Place of publication: | Weinheim |
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Document Type: | Article |
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Language: | English |
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Date of Publication (online): | 2019/11/06 |
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Date of first Publication: | 2019/11/06 |
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Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
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Release Date: | 2022/01/25 |
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Tag: | bioactivity testing; easyPACId; natural products; proteobacteria; simplified production |
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Volume: | 58 |
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Issue: | 52 |
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Page Number: | 7 |
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First Page: | 18957 |
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Last Page: | 18963 |
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Note: | Research Funding: LOEWE-Centre Translational Biodiversity Genomics. Grant Number: LOEWE TBG; Scientific and Technological Research Council of Turkey (TUBITAK). Grant Number: TOVAG-117O172; LOEWE-Centre Translational Medicine and Pharmacology. Grant Number: LOEWE TMP; LOEWE-Centre Insect Biotechnology and Bioressources. Grant Number: LOEWE Insect biotechnology; LOEWE research cluster MegaSyn. Grant Number: LOEWE MegaSyn; DFG. Grant Number: INST 161/810-1. |
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HeBIS-PPN: | 490966950 |
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Institutes: | Biowissenschaften |
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Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
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| 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
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Sammlungen: | Universitätspublikationen |
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Licence (German): | Creative Commons - Namensnennung 4.0 |
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