Switching at the ribosome: riboswitches need rProteins as modulators to regulate translation
- Translational riboswitches are cis-acting RNA regulators that modulate the expression of genes during translation initiation. Their mechanism is considered as an RNA-only gene-regulatory system inducing a ligand-dependent shift of the population of functional ON- and OFF-states. The interaction of riboswitches with the translation machinery remained unexplored. For the adenine-sensing riboswitch from Vibrio vulnificus we show that ligand binding alone is not sufficient for switching to a translational ON-state but the interaction of the riboswitch with the 30S ribosome is indispensable. Only the synergy of binding of adenine and of 30S ribosome, in particular protein rS1, induces complete opening of the translation initiation region. Our investigation thus unravels the intricate dynamic network involving RNA regulator, ligand inducer and ribosome protein modulator during translation initiation.
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| Author: | Vanessa de JesusORCiDGND, Nusrat QureshiGND, Sven WarhautGND, Jasleen Kaur BainsORCiDGND, Marina DietzORCiDGND, Mike HeilemannORCiDGND, Harald SchwalbeORCiDGND, Boris FürtigORCiDGND |
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| URN: | urn:nbn:de:hebis:30:3-799680 |
| DOI: | https://doi.org/10.1038/s41467-021-25024-5 |
| ISSN: | 2041-1723 |
| Parent Title (English): | Nature Communications |
| Publisher: | Nature Publishing Group UK |
| Place of publication: | [London] |
| Document Type: | Article |
| Language: | English |
| Date of Publication (online): | 2021/08/05 |
| Date of first Publication: | 2021/08/05 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2024/01/11 |
| Tag: | RNA; Solution-state NMR |
| Volume: | 12 |
| Issue: | art. 4723 |
| Article Number: | 4723 |
| Page Number: | 7 |
| First Page: | 1 |
| Last Page: | 7 |
| Note: | The work was supported by funds from DFG in collaborative research center 902: “Molecular principles of RNA-based regulation” and in Graduate College GRK1986 (CLIC). Work at BMRZ is supported by the state of Hesse (HMWK). Open Access funding enabled and organized by Projekt DEAL. |
| Institutes: | Biochemie, Chemie und Pharmazie |
| Wissenschaftliche Zentren und koordinierte Programme / Zentrum für Biomolekulare Magnetische Resonanz (BMRZ) | |
| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
| 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie | |
| Sammlungen: | Universitätspublikationen |
| Licence (German): |  Creative Commons - CC BY - Namensnennung 4.0 International |
