Cox2 community barcoding at Prince Edward Island reveals long-distance dispersal of a downy mildew species and potentially marine members of the Saprolegniaceae

  • Marine oomycetes are highly diverse, globally distributed, and play key roles in marine food webs as decomposers, food source, and parasites. Despite their potential importance in global ocean ecosystems, marine oomycetes are comparatively little studied. Here, we tested if the primer pair cox2F_Hud and cox2-RC4, which is already well-established for phylogenetic investigations of terrestrial oomycetes, can also be used for high-throughput community barcoding. Community barcoding of a plankton sample from Brudenell River (Prince Edward Island, Canada), revealed six distinct oomycete OTU clusters. Two of these clusters corresponded to members of the Peronosporaceae—one could be assigned to Peronospora verna, an obligate biotrophic pathogen of the terrestrial plant Veronica serpyllifolia and related species, the other was closely related to Globisporangium rostratum. While the detection of the former in the sample is likely due to long-distance dispersal from the island, the latter might be a bona fide marine species, as several cultivable species of the Peronosporaceae are known to withstand high salt concentrations. Two OTU lineages could be assigned to the Saprolegniaceae. While these might represent marine species of the otherwise terrestrial genus, it is also conceivable that they were introduced on detritus from the island. Two additional OTU clusters were grouped with the early-diverging oomycete lineages but could not be assigned to a specific family. This reflects the current underrepresentation of cox2 sequence data which will hopefully improve with the increasing interest in marine oomycetes.
Author:Julia A. F. Langer, Rahul SharmaGND, Bora NamGND, Louis Hanic, Maarten Boersma, Klaus SchwenkORCiD, Marco ThinesORCiDGND
Parent Title (English):Mycological progress
Place of publication:Berlin ; Heidelberg
Document Type:Article
Date of Publication (online):2021/04/16
Date of first Publication:2021/04/16
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2022/07/12
Tag:Brudenell River; Canada; Community barcoding; Marine ecosystem; Oomycetes; Peronosporaceae; cox2
Page Number:8
First Page:509
Last Page:516
Open Access funding enabled and organized by Projekt DEAL. This study was part of the BMBF (Bundesministerium für Bildung und Forschung)-financed BIOACID II project, consortium 1, work package 1.14 (FKZ 03F0655B). MT is supported by LOEWE in the framework of the Centre for Translational Biodiversity Genomics.
Fachübergreifende Einrichtungen / Biodiversität und Klima Forschungszentrum (BiK-F)
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Licence (German):License LogoCreative Commons - Namensnennung 4.0