Acute ethanol gavage attenuates hemorrhage/resuscitation-induced hepatic oxidative stress in rats
- Acute ethanol intoxication increases the production of reactive oxygen species (ROS). Hemorrhagic shock with subsequent resuscitation (H/R) also induces ROS resulting in cellular and hepatic damage in vivo. We examined the role of acute ethanol intoxication upon oxidative stress and subsequent hepatic cell death after H/R. 14 h before H/R, rats were gavaged with single dose of ethanol or saline (5 g/kg, EtOH and ctrl; H/R_EtOH or H/R_ctrl, resp.). Then, rats were hemorrhaged to a mean arterial blood pressure of 30 ± 2 mmHg for 60 min and resuscitated. Two control groups underwent surgical procedures without H/R (sham_ctrl and sham_EtOH, resp.). Liver tissues were harvested at 2, 24, and 72 h after resuscitation. EtOH-gavage induced histological picture of acute fatty liver. Hepatic oxidative (4-hydroxynonenal, 4-HNE) and nitrosative (3-nitrotyrosine, 3-NT) stress were significantly reduced in EtOH-gavaged rats compared to controls after H/R. Proapoptotic caspase-8 and Bax expressions were markedly diminished in EtOH-gavaged animals compared with controls 2 h after resuscitation. EtOH-gavage increased antiapoptotic Bcl-2 gene expression compared with controls 2 h after resuscitation. iNOS protein expression increased following H/R but was attenuated in EtOH-gavaged animals after H/R. Taken together, the data suggest that acute EtOH-gavage may attenuate H/R-induced oxidative stress thereby reducing cellular injury in rat liver.
Author: | Borna ReljaORCiDGND, Kerstin WilhelmORCiDGND, Minhong Wang, Dirk HenrichORCiDGND, Ingo MarziORCiDGND, Mark LehnertGND |
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URN: | urn:nbn:de:hebis:30:3-250164 |
DOI: | https://doi.org/10.1155/2012/983427 |
ISSN: | 1942-0994 |
ISSN: | 1942-0900 |
Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/22550557 |
Parent Title (English): | Oxidative medicine and cellular longevity |
Publisher: | Hindawi |
Place of publication: | New York, NY |
Document Type: | Article |
Language: | English |
Date of Publication (online): | 2012/04/05 |
Date of first Publication: | 2012/04/05 |
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Release Date: | 2012/06/04 |
Volume: | Volume 2012 (2012) |
Issue: | Article ID 983427 |
Page Number: | 10 |
HeBIS-PPN: | 357397207 |
Institutes: | Medizin / Medizin |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Sammlungen: | Universitätspublikationen |
Licence (German): | Creative Commons - Namensnennung 3.0 |