A comprehensive insight into the lipid composition of Myxococcus xanthus by UPLC-ESI-MS
- Analysis of whole cell lipid extracts of bacteria by means of ultra-performance (UP)LC-MS allows a comprehensive determination of the lipid molecular species present in the respective organism. The data allow conclusions on its metabolic potential as well as the creation of lipid profiles, which visualize the organism's response to changes in internal and external conditions. Herein, we describe: i) a fast reversed phase UPLC-ESI-MS method suitable for detection and determination of individual lipids from whole cell lipid extracts of all polarities ranging from monoacylglycerophosphoethanolamines to TGs; ii) the first overview of a wide range of lipid molecular species in vegetative Myxococcus xanthus DK1622 cells; iii) changes in their relative composition in selected mutants impaired in the biosynthesis of α-hydroxylated FAs, sphingolipids, and ether lipids; and iv) the first report of ceramide phosphoinositols in M. xanthus, a lipid species previously found only in eukaryotes.
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| Author: | Wolfram LorenzenGND, Kenan A. J. BozhüyükORCiDGND, Niña S. CortinaORCiD, Helge Björn BodeORCiDGND |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-769746 |
| DOI: | https://doi.org/10.1194/jlr.M054593 |
| ISSN: | 0022-2275 |
| Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/25332432 |
| Parent Title (English): | Journal of lipid research |
| Publisher: | American Society for Biochemistry and Molecular Biology Publications |
| Place of publication: | Bethesda, Md |
| Document Type: | Article |
| Language: | English |
| Date of Publication (online): | 2021/01/04 |
| Year of first Publication: | 2014 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2024/02/09 |
| Tag: | ceramide phosphoinositols; electrospray ionization-mass spectrometry; ether lipids; lipid profiles; lipidomics; myxobacteria; reversed phase; ultra-performance liquid chromatography |
| Volume: | 55.2014 |
| Issue: | 12 |
| Page Number: | 14 |
| First Page: | 2620 |
| Last Page: | 2633 |
| Institutes: | Biowissenschaften / Biowissenschaften |
| Exzellenzcluster / Exzellenzcluster Makromolekulare Komplexe | |
| Fachübergreifende Einrichtungen / Buchmann Institut für Molekulare Lebenswissenschaften (BMLS) | |
| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
| Sammlungen: | Universitätspublikationen |
| Licence (German): |  Creative Commons - CC BY - Namensnennung 4.0 International |
