Planar asymmetries in the C. elegans embryo emerge by differential retention of apars at cell-cell contacts

  • Formation of the anteroposterior and dorsoventral body axis in Caenorhabditis elegans depends on cortical flows and advection of polarity determinants. The role of this patterning mechanism in tissue polarization after formation of cell-cell contacts is not fully understood. Here, we demonstrate that planar asymmetries are established during left-right symmetry breaking: Centripetal cortical flows asymmetrically and differentially advect anterior polarity determinants (aPARs) from contacts to the medial cortex, resulting in their unmixing from apical myosin. Contact localization and advection of PAR-6 requires balanced CDC-42 activation, while asymmetric retention and advection of PAR-3 can occur independently of PAR-6. Concurrent asymmetric retention of PAR-3, E-cadherin/HMR-1 and opposing retention of antagonistic CDC-42 and Wnt pathway components leads to planar asymmetries. The most obvious mark of planar asymmetry, retention of PAR-3 at a single cell-cell contact, is required for proper cytokinetic cell intercalation. Hence, our data uncover how planar polarity is established in a system without the canonical planar cell polarity pathway through planar asymmetric retention of aPARs.

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Author:Priyanka Dutta, Devang Odedra, Christian Pohl
URN:urn:nbn:de:hebis:30:3-515247
DOI:https://doi.org/10.3389/fcell.2019.00209
ISSN:2296-634X
Pubmed Id:https://pubmed.ncbi.nlm.nih.gov/31612135
Parent Title (English):Frontiers in cell and developmental biology
Publisher:Frontiers Media
Place of publication:Lausanne
Contributor(s):Shamik Sen
Document Type:Article
Language:English
Year of Completion:2019
Date of first Publication:2019/09/27
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2019/10/30
Tag:CDC-42; PAR complex; Wnt; asymmetry; cortical flow; morphogenesis; planar polarity
Volume:7
Issue:Art. 209
Page Number:20
First Page:1
Last Page:20
Note:
Copyright © 2019 Dutta, Odedra and Pohl. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
HeBIS-PPN:455690456
Institutes:Biochemie, Chemie und Pharmazie / Biochemie und Chemie
Exzellenzcluster / Exzellenzcluster Makromolekulare Komplexe
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften
5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Sammlungen:Universitätspublikationen
Sammlung Biologie / Sondersammelgebiets-Volltexte
Open-Access-Publikationsfonds:Biowissenschaften
Licence (German):License LogoCreative Commons - Namensnennung 4.0