Refine
Year of publication
- 2016 (730) (remove)
Document Type
- Article (730) (remove)
Language
- English (730) (remove)
Has Fulltext
- yes (730)
Is part of the Bibliography
- no (730)
Keywords
- Mitochondria (7)
- crystal structure (7)
- invasion (5)
- Cortex (4)
- Gender (4)
- Invasive species (4)
- apoptosis (4)
- cancer (4)
- management (4)
- Apoptosis (3)
Institute
- Medizin (283)
- Biowissenschaften (70)
- Physik (69)
- Geowissenschaften (61)
- Frankfurt Institute for Advanced Studies (FIAS) (58)
- Biochemie und Chemie (46)
- Informatik (38)
- Biodiversität und Klima Forschungszentrum (BiK-F) (34)
- Institut für Ökologie, Evolution und Diversität (27)
- Senckenbergische Naturforschende Gesellschaft (27)
Kinetics of circulating endothelial progenitor cells in patients undergoing carotid artery surgery
(2016)
Aim: Endothelial progenitor cells (EPCs) are primitive cells found in the bone marrow and peripheral blood (PB). In particular, the potential of EPCs to differentiate into mature endothelial cells remains of high interest for clinical applications such as bio-functionalized patches for autologous seeding after implantation. The objective of this study was to determine EPCs’ kinetics in patients undergoing carotid artery thromboendarterectomy (CTEA) and patch angioplasty.
Methods: Twenty CTEA patients were included (15 male, mean age 76 years). PB samples were taken at 1 day preoperatively, and at 1, 3, and 5 days postoperatively. Flow cytometric analysis was performed for CD34, CD133, KDR, and CD45. Expression of KDR, SDF-1α, and G-CSF was analyzed by means of enzyme-linked immunosorbent assay.
Results: Fluorescence-activated cell sorting analysis revealed 0.031%±0.016% (% of PB mononuclear cells) KDR+ cells and 0.052%±0.022% CD45-/CD34+/CD133+ cells, preoperatively. A 33% decrease of CD45–/CD34+/CD133+ cells was observed at day 1 after surgery. However, a relative number (compared to initial preoperative values) of CD45-/CD34+/CD133+ cells was found on day 3 (82%) and on day 5 (94%) postoperatively. More profound upregulated levels of CD45–CD34+/CD133+ cells were observed for diabetic (+47% compared to nondiabetic) and male (+38% compared to female) patients. No significant postoperative time-dependent differences were found in numbers of KDR+ cells and the concentrations of the cytokines KDR and G-CSF. However, the SDF-1α levels decreased significantly on day 1 postoperatively but returned to preoperative levels by day 3.
Conclusion: CTEA results in short-term downregulation of circulating EPCs and SDF-1α levels. Rapid return to baseline levels might indicate participation of EPCs in repair mechanisms following vascular injury.
This article uses survey data from the German Socio-Economic Panel (GSOEP) to analyze the persistence of educational attainment across three generations in Germany. I obtain evidence of a robust effect of grandparents’ education on respondents’ own educational attainment in West Germany, net of parental class, education, occupational status, family income, parents’ relationship history, and family size. I also test whether the grandparent effect results from resource compensation or cumulative advantage and find empirical support for both mechanisms. In comparison, the intergenerational association between grandparents’ and respondents’ education is considerably weaker in East Germany and is also mediated completely by parental education. There are hardly any gender differences in the role of grandparents for respondents’ educational attainment, except for the fact that resource compensation is found to be exclusively relevant for women’s attainment in both West Germany and in East Germany after German reunification and the associated transition to an open educational system.
Aim: The purpose of this study was to analyze treatment compliance in osteoporotic patients treated with osteoporosis medications in Germany.
Methods: Patients included in the analysis had been diagnosed with osteoporosis with or without fractures and started anti-osteoporotic therapy (bisphosphonates, denosumab, or strontium ranelate) between 2011 and 2014 in a general (GP) or orthopedic practice (OP) setting in Germany. Data pertaining to 6,221 individuals followed in GP and 4,044 individuals followed in OP were analyzed retrospectively. The last follow-up was in December 2015. The main outcome measure was the compliance within the one-year period after the index prescription date. Compliance was measured indirectly and was based on the mean possession ratio (MPR). A multivariate logistic regression model was used to determine the association between MPR (dependent variable) and age, gender, type of practice, type of osteoporosis treatment, therapy frequency, and history of fracture (covariates).
Results: The mean age of the study group was 73.3 years, and 13.2% of subjects were men. Regarding type of practice, 60.6% of individuals were followed in GP and 39.4% in OP. Noncompliance was observed in 55.2% of the patients. Patients in the age group ≤60 years were at a higher risk of being noncompliant when compared to those in the age group of 61–70 years. Men and patients who received oral drugs were also more likely to be noncompliant than women and patients who received injectable or intravenous drugs. Finally, therapies that were given every three or six months were associated with a decrease in the risk of noncompliance when compared to weekly therapy, whereas daily and monthly treatments were associated with an increased risk.
Conclusion: Compliance is insufficient in osteoporotic patients treated with osteoporosis medications.
Introduction: Gravity plays a pivotal role in the pathogenesis of ventilator-associated pneumonia (VAP) (1). In previous laboratory studies (2) the semi-lateral Trendelenburg position (LTP) hindered gravity-driven pulmonary aspiration and avoided VAP.
Objectives: To determine whether the LTP vs. the semi-recumbent position (SRP) would reduce the incidence of microbiologically confirmed VAP and to appraise patient's compliance and safety.
Methods: We conducted a randomized, single-blind, controlled study in 17 European centers and 1 in North America. A total of 2019 adult patients were screened between 2010 and 2015. 395 patients were randomized - 194 in LTP and 201 in SRP - and analyzed in an intention to treat approach. Patients in LTP were placed in semi-lateral (60°) - Trendelenburg position to achieve an orientation, from the sternal notch toward the mouth, slightly below horizontal, and turned from one side to the other every 6 hours. LTP was encouraged during the first days of mechanical ventilation, but always in compliance with the patient's wish. In the SRP group, the head of the bed was elevated ≥ 30°. Primary outcome was VAP incidence rate, based on quantitative bronchoalveolar lavage fluid culture with ≥ 104 colonyforming units/mL. Secondary outcomes were compliance to the randomized position, length of intubation, duration of intensive care unit and hospital stay, mortality, and adverse events.
Results: The trial was stopped after the planned interim analysis for achieving efficacy endpoints and owing to safety concerns. Patients in the LTP and SRP group were kept in the randomized position for 38 % and 90 % of the study time, respectively (p = 0.001). Yet, during the first 48 hours, LTP patients were kept in the randomized position for 50 % of the study time, and SRP patients for 88 % (p = 0.001). In the LTP, the bed was angulated 5.6° in Trendelenburg; while, the head of the bed was elevated 34.1° in the SRP group. Incidence rates of microbiologically confirmed VAP were 0.88 (1/1136 patient-days; 95 % confidence interval [CI], 0.12-6.25) in the LTP group, and 7.19 (8/1113 patient-days; CI 95 %, 3.60-14.37) in the SRP (p = 0.020), relative risk reduction of 0.12 (95 % CI, 0.01-0.91). No statistically significant differences were observed in durations of mechanical ventilation, intensive care unit and hospital stay, and mortality. Vomiting was more common in LTP patients (8.3 % vs. 2.5 % in the SRP, p = 0.013).
Conclusions: Critically ill patients positioned in the LTP had a statistically significant reduction in the incidence of VAP, compared with those positioned in the SRP. A comprehensive evaluation of potential LTP contraindications is warranted to enhance safety.
When I first wrote about linguistic self-defense (discussed in Liav Orgad’s book pp. 198-200) I had a conception of languages in danger, The most visible potential victim were the French in Quebec. But with the help of Charles de Gaulle, the Quebecois have held on well to their culture (majority at home, minority at large, but supported by a large nation in Europe). One form of linguistic self-defense I proposed at the time was insisting on speaking your language in commercial transactions. For the sake of profit, store keepers would play along. Also, public advertising is a critical mode of making a language seem like the background state of normalcy. The key case in Quebec, as I recall, was called Chaussures Brown Shoes. That was the way they wanted their sign to read. The Anglophones objected and lost.
Does the Polish development concern us — the European citizens and the European institutions we have set up? There is a functional and a normative argument to state that it does. The normative argument is that the European Union organizes a community of states that profess allegiance to a set of fundamental values—among others, democracy, the rule of law, and human rights. The functional reason is that the European legal space presupposes mutual trust. European law operates on the presumption that all institutions are law-abiding. Otherwise, the legal edifice crumbles.
The application of the EU Commission’s Rule of Law Framework in the current Polish case is a step in the right direction. It seems a good instance to develop the Framework as an EU mechanism to protect European constitutional values in a European legal space which is rife with constitutional crises, but short of instruments to address them. Its pertinence appears even more clearly in comparison to the Council’s (in)activity under its own rule-of-law mechanism, hastily put forward after the Commission’s Framework. The activation of the Framework has shown its potential to mobilize European public opinion and orient public discourses to the current condition of EU values
Progranulin deficiency is associated with neurodegeneration in humans and in mice. The mechanisms likely involve progranulin-promoted removal of protein waste via autophagy. We performed a deep proteomic screen of the pre-frontal cortex in aged (13–15 months) female progranulin-deficient mice (GRN−/−) and mice with inducible neuron-specific overexpression of progranulin (SLICK-GRN-OE) versus the respective control mice. Proteins were extracted and analyzed per liquid chromatography/mass spectrometry (LC/MS) on a Thermo Scientific™ Q Exactive Plus equipped with an ultra-high performance liquid chromatography unit and a Nanospray Flex Ion-Source. Full Scan MS-data were acquired using Xcalibur and raw files were analyzed using the proteomics software Max Quant. The mouse reference proteome set from uniprot (June 2015) was used to identify peptides and proteins. The DiB data file is a reduced MaxQuant output and includes peptide and protein identification, accession numbers, protein and gene names, sequence coverage and label free quantification (LFQ) values of each sample. Differences in protein expression in genotypes are presented in "Progranulin overexpression in sensory neurons attenuates neuropathic pain in mice: Role of autophagy" (C. Altmann, S. Hardt, C. Fischer, J. Heidler, H.Y. Lim, A. Haussler, B. Albuquerque, B. Zimmer, C. Moser, C. Behrends, F. Koentgen, I. Wittig, M.H. Schmidt, A.M. Clement, T. Deller, I. Tegeder, 2016) [1].
The data provided is related to the article "Phylogenetic analyses of gazelles reveal repeated transitions of key ecological traits and provide novel insights into the origin of the genus Gazella". The data is based on 48 tissue samples of all nine extant species of the genus Gazella, namely Gazella gazella, Gazella arabica, Gazella bennettii, Gazella cuvieri, Gazella dorcas, Gazella leptoceros, Gazella marica, Gazella spekei, and Gazella subgutturosa and four related taxa (Saiga tatarica, Antidorcas marsupialis, Antilope cervicapra and Eudorcas rufifrons). It comprises alignments of sequences of a cytochrome b data set and of six nuclear intron markers. For the latter new primers were designed based on cattle and sheep genomes. Based on these alignments phylogenetic trees were inferred using Bayesian Inference and Maximum Likelihood methods. Furthermore, ancestral character states (inferred with BayesTraits 1.0) and ancestral ranges based on a Dispersal-Extinction-Cladogenesis model were estimated and results׳ files were stored within this article.
The presence of cerebral lesions in patients with neurosensory alterations provides a unique window into brain function. Using a fuzzy logic based combination of morphological information about 27 olfactory-eloquent brain regions acquired with four different brain imaging techniques, patterns of brain damage were analyzed in 127 patients who displayed anosmia, i.e., complete loss of the sense of smell (n = 81), or other and mechanistically still incompletely understood olfactory dysfunctions including parosmia, i.e., distorted perceptions of olfactory stimuli (n = 50), or phantosmia, i.e., olfactory hallucinations (n = 22). A higher prevalence of parosmia, and as a tendency also phantosmia, was observed in subjects with medium overall brain damage. Further analysis showed a lower frequency of lesions in the right temporal lobe in patients with parosmia than in patients without parosmia. This negative direction of the differences was unique for parosmia. In anosmia, and also in phantosmia, lesions were more frequent in patients displaying the respective symptoms than in those without these dysfunctions. In anosmic patients, lesions in the right olfactory bulb region were much more frequent than in patients with preserved sense of smell, whereas a higher frequency of carriers of lesions in the left frontal lobe was observed for phantosmia. We conclude that anosmia, and phantosmia, are the result of lost function in relevant brain areas whereas parosmia is more complex, requiring damaged and intact brain regions at the same time.
The paper broaches the issue of unfair trading practices (UTPs) at the expense of, economically spoken, weaker actors among the food supply chain in context of the EU. For illustrating the concept of UTPs and delivering a theoretical basis for scrutinizing the term of fairness in respective trading practices the paper suggests the three variables 1) bargaining power, 2) market power/anti competitive practices and 3) unequal gain distribution. Subsequently the article presents selected national food-specific legislative based reactions towards UTPs evolved in context of the three variables. Ultimately the paper presents a qualitatively generated hypothesis which presumes that legislative food-specific measurements focussing on protecting suppliers lead to a beneficial monetary share for farmers, by means of influencing the producer price to a monetarily advantageous extent. The hypothesis was generated unprejudiced in the run-up to the paper. The research design which led to the hypothesis mentioned will be presented.
A generalized teleparallel cosmological model, f(TG,T), containing the torsion scalar T and the teleparallel counterpart of the Gauss–Bonnet topological invariant TG, is studied in the framework of the Noether symmetry approach. As f(G,R) gravity, where G is the Gauss–Bonnet topological invariant and R is the Ricci curvature scalar, exhausts all the curvature information that one can construct from the Riemann tensor, in the same way, f(TG,T) contains all the possible information directly related to the torsion tensor. In this paper, we discuss how the Noether symmetry approach allows one to fix the form of the function f(TG,T) and to derive exact cosmological solutions.
This letter reports on how the Wilson flow technique can efficaciously kill the short-distance quantum fluctuations of 2- and 3-gluon Green functions, remove the ΛQCD scale and destroy the transition from the confining non-perturbative to the asymptotically-free perturbative sector. After the Wilson flow, the behavior of the Green functions with momenta can be described in terms of the quasi-classical instanton background. The same behavior also occurs, before the Wilson flow, at low-momenta. This last result permits applications as, for instance, the detection of instanton phenomenological properties or a determination of the lattice spacing only from the gauge sector of the theory.
We report on new results on the infrared behavior of the three-gluon vertex in quenched Quantum Chromodynamics, obtained from large-volume lattice simulations. The main focus of our study is the appearance of the characteristic infrared feature known as ‘zero crossing’, the origin of which is intimately connected with the nonperturbative masslessness of the Faddeev–Popov ghost. The appearance of this effect is clearly visible in one of the two kinematic configurations analyzed, and its theoretical origin is discussed in the framework of Schwinger–Dyson equations. The effective coupling in the momentum subtraction scheme that corresponds to the three-gluon vertex is constructed, revealing the vanishing of the effective interaction at the exact location of the zero crossing.
Observations show that, at the beginning of their existence, neutron stars are accelerated briskly to velocities of up to a thousand kilometers per second. We argue that this remarkable effect can be explained as a manifestation of quantum anomalies on astrophysical scales. To theoretically describe the early stage in the life of neutron stars we use hydrodynamics as a systematic effective-field-theory framework. Within this framework, anomalies of the Standard Model of particle physics as underlying microscopic theory imply the presence of a particular set of transport terms, whose form is completely fixed by theoretical consistency. The resulting chiral transport effects in proto-neutron stars enhance neutrino emission along the internal magnetic field, and the recoil can explain the order of magnitude of the observed kick velocities.
We compare the reconstructed hadronization conditions in relativistic nuclear collisions in the nucleon–nucleon centre-of-mass energy range 4.7–2760 GeV in terms of temperature and baryon-chemical potential with lattice QCD calculations, by using hadronic multiplicities. We obtain hadronization temperatures and baryon chemical potentials with a fit to measured multiplicities by correcting for the effect of post-hadronization rescattering. The post-hadronization modification factors are calculated by means of a coupled hydrodynamical-transport model simulation under the same conditions of approximate isothermal and isochemical decoupling as assumed in the statistical hadronization model fits to the data. The fit quality is considerably better than without rescattering corrections, as already found in previous work. The curvature of the obtained “true” hadronization pseudo-critical line κ is found to be 0.0048 ± 0.0026, in agreement with lattice QCD estimates; the pseudo-critical temperature at vanishing is found to be 164.3 ± 1.8 MeV.
We discuss different models for the spin structure of the nonperturbative pomeron: scalar, vector, and rank-2 symmetric tensor. The ratio of single-helicity-flip to helicity-conserving amplitudes in polarised high-energy proton–proton elastic scattering, known as the complex r5 parameter, is calculated for these models. We compare our results to experimental data from the STAR experiment. We show that the spin-0 (scalar) pomeron model is clearly excluded by the data, while the vector pomeron is inconsistent with the rules of quantum field theory. The tensor pomeron is found to be perfectly consistent with the STAR data.
Recently the LIGO and VIRGO Collaborations reported the observation of gravitational-wave signal corresponding to the inspiral and merger of two black holes, resulting into formation of the final black hole. It was shown that the observations are consistent with the Einstein theory of gravity with high accuracy, limited mainly by the statistical error. Angular momentum and mass of the final black hole were determined with rather large allowance of tens of percents. Here we shall show that this indeterminacy in the range of the black-hole parameters allows for some non-negligible deformations of the Kerr spacetime leading to the same frequencies of the black-hole ringing. This means that at the current precision of the experiment there remains some possibility for alternative theories of gravity.
We study the effect of thermal charm production on charmonium regeneration in high energy nuclear collisions. By solving the kinetic equations for charm quark and charmonium distributions in Pb+Pb collisions, we calculate the global and differential nuclear modification factors RAA(Npart) and RAA(pt) for J/ψ s. Due to the thermal charm production in hot medium, the charmonium production source changes from the initially created charm quarks at SPS, RHIC and LHC to the thermally produced charm quarks at Future Circular Collider (FCC), and the J/ψ suppression (RAA<1) observed so far will be replaced by a strong enhancement (RAA>1) at FCC at low transverse momentum.
In the present work we study the effect of unparticle modified static potentials on the energy levels of the hydrogen atom. By using Rayleigh–Schrödinger perturbation theory, we obtain the energy shift of the ground state and compare it with experimental data. Bounds on the unparticle energy scale U as a function of the scaling dimension and the coupling constant λ are derived. We show that there exists a parameter region where bounds on U ar are stringent, signaling that unparticles could be tested in atomic physics experiments.
Several members of the genus Legionella cause Legionnaires’ disease, a potentially debilitating form of pneumonia. Studies frequently focus on the abundant number of virulence factors present in this genus. However, what is often overlooked is the role of secondary metabolites from Legionella. Following whole genome sequencing, we assembled and annotated the Legionella parisiensis DSM 19216 genome. Together with 14 other members of the Legionella, we performed comparative genomics and analysed the secondary metabolite potential of each strain. We found that Legionella contains a huge variety of biosynthetic gene clusters (BGCs) that are potentially making a significant number of novel natural products with undefined function. Surprisingly, only a single Sfp-like phosphopantetheinyl transferase is found in all Legionella strains analyzed that might be responsible for the activation of all carrier proteins in primary (fatty acid biosynthesis) and secondary metabolism (polyketide and non-ribosomal peptide synthesis). Using conserved active site motifs, we predict some novel compounds that are probably involved in cell-cell communication, differing to known communication systems. We identify several gene clusters, which may represent novel signaling mechanisms and demonstrate the natural product potential of Legionella.
Infective endocarditis (IE) is a life-threatening disease that is associated with high morbidity and mortality. Its long-term prognosis strongly depends on a timely and optimized antibiotic treatment. Therefore, identification of the causative pathogen is crucial and currently based on blood cultures followed by characterization and susceptibility testing of the isolate. However, antibiotic treatment starting prior to blood sampling or IE caused by fastidious or intracellular microorganisms may cause negative culture results. Here we investigate the additional diagnostic value of broad-range PCR in combination with direct sequencing on resected heart tissue or swabs in patients with tissue or swab culture-negative IE in a routine clinical setting. Sensitivity, specificity, and positive and negative predictive values of broad-range PCR from diagnostic material in our patients were 33.3%, 76.9%, 90.9%, and 14.3%, respectively. We identified a total of 20 patients (21.5%) with tissue or culture-negative IE who profited by the additional application of broad-range PCR. We conclude that broad-range PCR on resected heart tissue or swabs is an important complementary diagnostic approach. It should be seen as an indispensable new tool for both the therapeutic and diagnostic management of culture-negative IE and we thus propose its possible inclusion in Duke's diagnostic classification scheme.
The gut microbiome is significantly altered in inflammatory bowel diseases, but the basis of these changes is not well understood. We have combined metagenomic and metatranscriptomic profiling of the gut microbiome to assess modifications to both bacterial community structure and transcriptional activity in a mouse model of colitis. By using transcriptomic analysis of colonic tissue and luminal RNA derived from the host, we have also characterised how host transcription relates to the microbial transcriptional response in inflammation. In colitis, increased abundance and transcription of diverse microbial gene families involved in responses to nutrient deprivation, antimicrobial peptide production and oxidative stress support an adaptation of multiple commensal genera to withstand a diverse set of environmental stressors in the inflammatory environment. These data are supported by a transcriptional signature of activated macrophages and granulocytes in the gut lumen during colitis, a signature that includes the transcription of the key antimicrobial genes S100a8 and S100a9 (calprotectin). Genes involved in microbial resistance to oxidative stress, including Dps/ferritin, Fe-dependent peroxidase and glutathione S-transferase were identified as changing to a greater extent at the level of transcription than would be predicted by DNA abundance changes, implicating a role for increased oxygen tension and/or host-derived reactive oxygen species in driving transcriptional changes in commensal microbes.
Microwave sensors in medical environments play a significant role due to the contact-less and non-invasive sensing mechanism to determine dielectric properties of tissue. In this work, a theranostic sensor based on Split Ring Resonators (SRRs) is presented that provides two operation modes to detect and treat tumor cells, exemplary in the liver. For the detection mode, resonance frequency changes due to abnormalities are evaluated, and in the treatment mode, microwave ablation is performed. The planar sensor structure can be integrated into a needle like a surgery tool that evokes challenges concerning size limitations and biocompatibility. To meet the size requirements and provide a reasonable operating frequency, properties of oval shaped SRRs are investigated. By elongating the radius of the SRR in one direction, the resonance frequency can be decreased significantly compared to circular SRR by a factor of two below 12 GHz. In order to validate the detection and treatment characteristics of the sensor, full wave simulations and measurements are examined. Clear resonance shifts are detected for loading the sensor structures with phantoms mimicking healthy and malignant tissue. For treatment mode evaluation, ex vivo beef liver tissue was ablated leading to a lesion zone 1.2 cm × 1 cm × 0.3 cm with a three minute exposure of maximum 2.1 W
Populism is widely thought to be in tension with liberal democracy. This article clarifies what exactly is problematic about populism from a liberal–democratic point of view and goes on to develop normative standards that allow us to distinguish between more and less legitimate forms of populism. The point of this exercise is not to dismiss populism in toto; the article strives for a more subtle result, namely, to show that liberal democracy can accommodate populism provided that the latter conforms to particular discursive norms. What the article calls a ‘liberal ethics of populism’ turns out to be closely bound up with a broader ethics of peoplehood, understood as a way of articulating who ‘the people’ are in a way that is compatible with liberal–democratic principles of political justification. Such an ethics, concludes the article, inevitably has a much wider audience than populist political actors: its addressees are all those who seek legitimately to exercise power in the name of the people.
"Che tempo, che tempo": Geology and Environment in Max Frisch’s 'Der Mensch erscheint im Holozän'
(2016)
Critical readings of Frisch’s Der Mensch erscheint im Holozän [Man in the Holocene] have tended to read its heterogeneous and inter-medial form as a code for the mental disintegration of its protagonist. This paper argues instead that this feature can be seen as a poetological engagement with geological and climatic timescales. Due to its hybrid form, the incorporation of a multiplicity of textual fragments and pictorial representations, the text undermines both conventional definitions of narrative and representations of nature. Holozän’s non-linear structure establishes an aesthetic of slowness that ushers in an awareness of the utterly different time schemes of geological and climatic processes. Furthermore, the importance of the material features, such as an interplay between text and image and the disconnected, paratactical arrangement of sentences mirrors the novel’s focus on natural phenomena. Frisch’s narrative establishes a poetics that tries to reach beyond the confinements of an anthropocentric perspective and thereby subverts the borders between culture and environment.
Cryptochromes are a ubiquitous group of blue-light absorbing flavoproteins that in the mammalian retina have an important role in the circadian clock. In birds, cryptochrome 1a (Cry1a), localized in the UV/violet-sensitive S1 cone photoreceptors, is proposed to be the retinal receptor molecule of the light-dependent magnetic compass. The retinal localization of mammalian Cry1, homologue to avian Cry1a, is unknown and it is open whether mammalian Cry1 is also involved in magnetic field sensing. To constrain the possible role of retinal Cry1, we immunohistochemically analysed 90 mammalian species across 48 families in 16 orders, using an antiserum against the Cry1 C-terminus that in birds labels only the photo-activated conformation. In the Carnivora families Canidae, Mustelidae and Ursidae and in some Primates, Cry1 was consistently labeled in the outer segment of the shortwave-sensitive S1 cones. This finding would be compatible with a magnetoreceptive function of Cry1 in these taxa. In all other taxa, Cry1 was not detected by the antiserum that likely also in mammals labels the photo-activated conformation, although Western blots showed Cry1 in mouse retinal cell nuclei. We speculate that in the mouse and the other negative-tested mammals Cry1 is involved in circadian functions as a non-light-responsive protein.
Objective: Chronically HCV-infected orthotopic liver transplantation (OLT) recipients appear to have improved outcomes when their immunosuppressive regimen includes a mammalian target of rapamycin (mTOR) inhibitor. The mechanism underlying this observation is unknown.
Design: We used virological assays to investigate mTOR signalling on the HCV replication cycle. Furthermore, we analysed HCV RNA levels of 42 HCV-positive transplanted patients treated with an mTOR inhibitor as part of their immunosuppressive regimen.
Results: The mTOR inhibitor rapamycin was found to be a potent inhibitor for HCV RNA replication in Huh-7.5 cells as well as primary human hepatocytes. Half-maximal inhibition was observed at 0.01 µg/mL, a concentration that is in the range of serum levels seen in transplant recipients and does not affect cell proliferation. Early replication cycle steps such as cell entry and RNA translation were not affected. Knockdown of raptor, an essential component of mTORC1, but not rictor, an essential component of mTORC2, inhibited viral RNA replication. In addition, overexpression of raptor led to higher viral RNA replication, demonstrating that mTORC1, but not mTORC2, is required for HCV RNA replication. In 42 HCV-infected liver-transplanted or kidney-transplanted patients who were switched to an mTOR inhibitor, we could verify that mTOR inhibition decreased HCV RNA levels in vivo.
Conclusions: Our data identify mTORC1 as a novel HCV replication factor. These findings suggest an underlying mechanism for the observed benefits of mTOR inhibition in HCV-positive OLT recipients and potentiate further investigation of mTOR-containing regimens in HCV-positive recipients of solid organ transplants.
One of the major challenges of allogeneic stem cell transplantation (allo-SCT) is to reduce the risk of graft-versus-host disease (GVHD) while boosting the graft-versus-leukemia (GVL) effect. The reconstitution of natural killer (NK) cells following allo-SCT is of notable interest due to their known capability to induce GVL without GVHD. Here, in this study, we investigate the association between the incidence and severity of acute graft-versus-host disease (aGVHD) and the early reconstitution of NK cell subsets following allo-SCT. We analyzed 342 samples from 107 patients using flow cytometry, with a focus on immature CD56high and mature cytotoxic CD56dim NK cells. Longitudinal analysis of immune reconstitution after allo-SCT showed that the incidence of aGVHD was associated with a delayed expansion of the entire NK cell population, in particular the CD56high subset. Notably, the disturbed reconstitution of the CD56high NK cells also correlated with the severity of aGVHD.
Following up on earlier investigations, the present research aims at validating the construct impostor phenomenon by taking other personality correlates into account and to examine whether the impostor phenomenon is a construct in its own right. In addition, gender effects as well as associations with dispositional working styles and strain are examined. In an online study we surveyed a sample of N = 242 individuals occupying leadership positions in different sectors. Confirmatory factor analyses provide empirical evidence for the discriminant validity of the impostor phenomenon. In accord with earlier studies we show that the impostor phenomenon is accompanied by higher levels of anxiety, dysphoric moods, emotional instability, a generally negative self-evaluation, and perfectionism. The study does not reveal any gender differences concerning the impostor phenomenon. With respect to working styles, persons with an impostor self-concept tend to show perfectionist as well as procrastinating behaviors. Moreover, they report being more stressed and strained by their work. In sum, the findings show that the impostor phenomenon constitutes a dysfunctional personality style. Practical implications are discussed.
Much is known about when children acquire an understanding of mental states, but few, if any, experiments identify social contexts in which children tend to use this capacity and dispositions that influence its usage. Social exclusion is a common situation that compels us to reconnect with new parties, which may crucially involve attending to those parties’ mental states. Across two studies, this line of inquiry was extended to typically developing preschoolers (Study 1) and young children with and without anxiety disorder (AD) (Study 2). Children played the virtual game of toss “Cyberball” ostensibly over the Internet with two peers who first played fair (inclusion), but eventually threw very few balls to the child (exclusion). Before and after Cyberball, children in both studies completed stories about peer-scenarios. For Study 1, 36 typically developing 5-year-olds were randomly assigned to regular exclusion (for no apparent reason) or accidental exclusion (due to an alleged computer malfunction). Compared to accidental exclusion, regular exclusion led children to portray story-characters more strongly as intentional agents (intentionality), with use of more mental state language (MSL), and more between-character affiliation in post-Cyberball stories. For Study 2, 20 clinically referred 4 to 8-year-olds with AD and 15 age- and gender-matched non-anxious controls completed stories before and after regular exclusion. While we replicated the post regular-exclusion increase of intentional and MSL portrayals of story-characters among non-anxious controls, anxious children exhibited a decline on both dimensions after regular exclusion. We conclude that exclusion typically induces young children to mentalize, enabling more effective reconnection with others. However, excessive anxiety may impair controlled mentalizing, which may, in turn, hamper effective reconnection with others after exclusion.
Understanding a sentence and integrating it into the discourse depends upon the identification of its focus, which, in spoken German, is marked by accentuation. In the case of written language, which lacks explicit cues to accent, readers have to draw on other kinds of information to determine the focus. We study the joint or interactive effects of two kinds of information that have no direct representation in print but have each been shown to be influential in the reader's text comprehension: (i) the (low-level) rhythmic-prosodic structure that is based on the distribution of lexically stressed syllables, and (ii) the (high-level) discourse context that is grounded in the memory of previous linguistic content. Systematically manipulating these factors, we examine the way readers resolve a syntactic ambiguity involving the scopally ambiguous focus operator auch (engl. “too”) in both oral (Experiment 1) and silent reading (Experiment 2). The results of both experiments attest that discourse context and local linguistic rhythm conspire to guide the syntactic and, concomitantly, the focus-structural analysis of ambiguous sentences. We argue that reading comprehension requires the (implicit) assignment of accents according to the focus structure and that, by establishing a prominence profile, the implicit prosodic rhythm directly affects accent assignment.
Background: Prophylactic mesh-augmented reinforcement during closure of abdominal wall incisions has been proposed in patients with increased risk for development of incisional hernias (IHs). As part of the BioMesh consensus project, a systematic literature review has been performed to detect those studies where MAR was performed with a non-permanent absorbable mesh (biological or biosynthetic).
Methods: A computerized search was performed within 12 databases (Embase, Medline, Web-of-Science, Scopus, Cochrane, CINAHL, Pubmed publisher, Lilacs, Scielo, ScienceDirect, ProQuest, Google Scholar) with appropriate search terms. Qualitative evaluation was performed using the MINORS score for cohort studies and the Jadad score for randomized clinical trials (RCTs).
Results: For midline laparotomy incisions and stoma reversal wounds, two RCTs, two case–control studies, and two case series were identified. The studies were very heterogeneous in terms of mesh configuration (cross linked versus non-cross linked), mesh position (intraperitoneal versus retro-muscular versus onlay), surgical indication (gastric bypass versus aortic aneurysm), outcome results (effective versus non-effective). After qualitative assessment, we have to conclude that the level of evidence on the efficacy and safety of biological meshes for prevention of IHs is very low. No comparative studies were found comparing biological mesh with synthetic non-absorbable meshes for the prevention of IHs.
Conclusion: There is no evidence supporting the use of non-permanent absorbable mesh (biological or biosynthetic) for prevention of IHs when closing a laparotomy in high-risk patients or in stoma reversal wounds. There is no evidence that a non-permanent absorbable mesh should be preferred to synthetic non-absorbable mesh, both in clean or clean-contaminated surgery.
This paper reflects on legal pluralism. How did medieval societies incorporate both unwritten customs and written law at the same time? How did they constitute the process of finding justice? What is the essense of legal pluralism, and will it help us understand the situation of Taiwan’s indigenous population?
We aim to solve these problems by taking a closer look at medieval Saxony: for around 400 years, both laws given by the authorities and traditional customs in Saxony worked fine in parallel. The latter were put into writing by the legal practitioner Eike von Repgow around 1230 for reasons unknown. We refer to his collection of laws and customs of the Saxons as the Sachsenspiegel ("Mirror of Saxons").
While Saxons certainly differed from Taiwan’s indigenous population for many reasons, such as the supposedly weaker egalitarianism among the Saxons than among at least some indigenous groups, the two show some remarkable similarities nonetheless. Just like the Taiwanese Gaya, the Sachsenspiegel’s spiritual origin raises the claim to validity. Furthermore, comparing the handling of a person’s sale of inherited property, the legal situations in the Sachsenspiegel and Taiwan’s unwritten customs resemble each other. The heir can transfer only property he acquired personally. Furthermore, the author discusses the different character of courts and procedure under oral law in contrast to written modern law.
Finally, the paper concludes with some remarks about a learned commentary on the Sachsenspiegel written around 1325, combined with an outlook on the possible future of Taiwanese customs.
Circulation of legal knowledge, ideas, norms and practices has taken place throughout legal history, shaping legal experiences in different corners of the world. Over the past couple of years, approaches to the study of such circulations have changed radically. Legal historians have adopted approaches from cultural studies and transnational history in order to gain a deeper understanding of the complexities inherent in these processes. ...
The graph theoretical analysis of structural magnetic resonance imaging (MRI) data has received a great deal of interest in recent years to characterize the organizational principles of brain networks and their alterations in psychiatric disorders, such as schizophrenia. However, the characterization of networks in clinical populations can be challenging, since the comparison of connectivity between groups is influenced by several factors, such as the overall number of connections and the structural abnormalities of the seed regions. To overcome these limitations, the current study employed the whole-brain analysis of connectional fingerprints in diffusion tensor imaging data obtained at 3 T of chronic schizophrenia patients (n = 16) and healthy, age-matched control participants (n = 17). Probabilistic tractography was performed to quantify the connectivity of 110 brain areas. The connectional fingerprint of a brain area represents the set of relative connection probabilities to all its target areas and is, hence, less affected by overall white and gray matter changes than absolute connectivity measures. After detecting brain regions with abnormal connectional fingerprints through similarity measures, we tested each of its relative connection probability between groups. We found altered connectional fingerprints in schizophrenia patients consistent with a dysconnectivity syndrome. While the medial frontal gyrus showed only reduced connectivity, the connectional fingerprints of the inferior frontal gyrus and the putamen mainly contained relatively increased connection probabilities to areas in the frontal, limbic, and subcortical areas. These findings are in line with previous studies that reported abnormalities in striatal–frontal circuits in the pathophysiology of schizophrenia, highlighting the potential utility of connectional fingerprints for the analysis of anatomical networks in the disorder.
Chronic or repeated stress, particularly psychosocial stress, is an acknowledged risk factor for numerous affective and somatic disorders in modern societies. Thus, there is substantial evidence showing that chronic stress can increase the likelihood of major depressive disorder and anxiety disorders, as well as cardiovascular diseases, irritable bowel syndrome and pain syndromes, to name but a few, in vulnerable individuals. Although a number of pharmacological agents are available to treat such stress-related disorders, many patients do not respond to them, and those who do often report a number of side effects. ...
This article compares the legislation promulgated by the Synod of Granada (1572) and the Third Mexican Provincial Council (1585) regarding procedural canonical law. Diego Romano, bishop of Puebla, served as a vehicle between the Spanish and Mexican Assemblies, and he was clearly inspired by the former when drafting the latter. The article pays attention to the level of appropriation and via a comparison of the texts addresses the question whether it is possible to say that Iberian procedural law was copied by the prelate.
Atmospheric aerosols and their effect on clouds are thought to be important for anthropogenic radiative forcing of the climate, yet remain poorly understood1. Globally, around half of cloud condensation nuclei originate from nucleation of atmospheric vapours2. It is thought that sulfuric acid is essential to initiate most particle formation in the atmosphere3,4, and that ions have a relatively minor role5. Some laboratory studies, however, have reported organic particle formation without the intentional addition of sulfuric acid, although contamination could not be excluded6,7. Here we present evidence for the formation of aerosol particles from highly oxidized biogenic vapours in the absence of sulfuric acid in a large chamber under atmospheric conditions. The highly oxygenated molecules (HOMs) are produced by ozonolysis of α-pinene. We find that ions from Galactic cosmic rays increase the nucleation rate by one to two orders of magnitude compared with neutral nucleation. Our experimental findings are supported by quantum chemical calculations of the cluster binding energies of representative HOMs. Ion-induced nucleation of pure organic particles constitutes a potentially widespread source of aerosol particles in terrestrial environments with low sulfuric acid pollution.
Cross education is the process whereby training of one limb gives rise to increases in the subsequent performance of its opposite counterpart. The execution of many unilateral tasks is associated with increased excitability of corticospinal projections from primary motor cortex (M1) to the opposite limb. It has been proposed that these effects are causally related. Our aim was to establish whether changes in corticospinal excitability (CSE) arising from prior training of the opposite limb determine levels of interlimb transfer. We used three vision conditions shown previously to modulate the excitability of corticospinal projections to the inactive (right) limb during wrist flexion movements performed by the training (left) limb. These were: (1) mirrored visual feedback of the training limb; (2) no visual feedback of either limb; and (3) visual feedback of the inactive limb. Training comprised 300 discrete, ballistic wrist flexion movements executed as rapidly as possible. Performance of the right limb on the same task was assessed prior to, at the mid point of, and following left limb training. There was no evidence that variations in the excitability of corticospinal projections (assessed by transcranial magnetic stimulation (TMS)) to the inactive limb were associated with, or predictive of, the extent of interlimb transfer that was expressed. There were however associations between alterations in muscle activation dynamics observed for the untrained limb, and the degree of positive transfer that arose from training of the opposite limb. The results suggest that the acute adaptations that mediate the bilateral performance gains realized through unilateral practice of this ballistic wrist flexion task are mediated by neural elements other than those within M1 that are recruited at rest by single-pulse TMS.
Thomas Reedy (1842-1929), gardener from 1854-1926 at Camden Park, the family estate of the Macarthurs in western Sydney was sent by Sir William Macarthur on the Chevert Expedition to New Guinea in 1875. He collected plants on islands off the Queensland coast; Cape Grenville; around the Somerset outpost on the tip of Cape York; on islands in the Torres Strait; and around two locations on the mainland of southern Papua New Guinea. Reedy sent or brought back 800-1000 living plants, in addition to an unknown number of seeds, and at least 157 dried specimens, the latter sent to Baron Ferdinand von Mueller in Melbourne. This study is focused on locating the dried plant specimens now held in herbaria and highlighting their significance.
Reedy is something of an enigma with little ever written about him. His initial has been presented erroneously since 1875 and has gone through various morphs including J, P, M and T; searches for Reedy best use all these initials or
just his family name.
Melichrus urceolatus R.Br. (family Ericaceae) (Urn heath) is a small shrub endemic to eastern Australia found in open grassy woodlands and heath from southern Queensland to central Victoria. Very little is known of its ecology, particularly in relation to its pollination and reproductive strategies. Most Melichrus species are thought to be animal-pollinated, but some floral traits of Melichrus urceolatus suggest wind pollination. Here, I describe an observation of parasitic wasps (Diapriidae sp.) found inside Melichrus urceolatus flowers in a Box-Gum Grassy Woodland remnant in Albury, New South Wales and discuss the ecological significance of the observation.
A survey of the population of the parasitic herb Thesium australe R.Br. (family Santalaceae) in Booroolong Nature Reserve, northwest of Armidale, found it was restricted to an eastern facing lower slope on metasediments, within regenerating grassy woodland of Eucalyptus nova-anglica H.Deane & Maiden (New England Peppermint) and a predominantly Themeda triandra Forssk. (Kangaroo Grass) and Sorghum leiocladum (Hack.) C.E.Hubb. (Native Sorghum) understorey. The population is in two clusters over a total area approximately 260 m long by 40 m wide and was estimated to be about 800 individuals at the time of survey (May 2014). Current threats include a maintenance trail, erosion of creek bank, stray cattle and sheep, rabbits and Rubus anglocandicans A.Newton (Blackberry) invasion. It is recommended that further searches be conducted and that monitoring occur on a regular basis using the permanently marked sites and methodology used in this survey.
Naree and Yantabulla stations (31,990 ha) are found 60 km south-east of Hungerford and 112 km north-west of Bourke, New South Wales (lat. 29° 55'S; long. 150°37'N). The properties occur on the Cuttaburra Creek within the Mulga Lands Bioregion. We describe the vegetation assemblages found on these properties within three hierarchical levels (Group, Alliance & Association). Vegetation levels are defined based on flexible UPGMA analysis of coverabundance scores of all vascular plant taxa. These vegetation units are mapped based on extensive ground truthing, SPOT5 imagery interpretation and substrate. Three ‘Group’ level vegetation types are described: Mulga Complex, Shrublands Complex and Floodplain Wetlands Complex. Within these Groups nine ‘Alliances’ are described: Rat’s tail Couch – Lovegrass Grasslands, Canegrass Grasslands, Lignum – Glinus Shrublands, Coolibah – Black Box Woodlands, Turpentine – Button Grass – Windmill Grass Shrublands, Turpentine – Hop Bush – Kerosene Grass shrublands and Mulga Shrublands. Sixteen ‘Associations’ are described 1) Mulga – Poplar Box Shrubland, 2) Mulga – Poplar Box – Bastard Mulga Shrubland, 3) Turpentine – Hop Bush – Senna Shrubland, 4) Turpentine – Elegant Wattle – Boobialla Shrubland, 5) Turpentine – Hop Bush – Daisy Bush Shrubland, 5) Belah – Rosewood – Turpentine Bush Shrubland, 6) Belah – Rosewood – Turpentine Bush Shrubland, 7) Ironwood – Leopardwood – Supplejack Shrubland, 8) Yapunyah – Black Box – River Cooba Woodland, 9) Coolibah – River Cooba – Yapunyah Woodland, 10) Rat’s tail Couch – Lovegrass – Fairy Grass Grassland and Herbfield, 11) Rat’s tail Couch – Lovegrass – Purslane Grassland and Herbfield, 12) Darling Pratia – Rat’s tail Couch – Spike Rush Herbfield, 13) Canegrass Grassland, 14) Glinus – Groundsel – Lignum Herbfield, 15) Poplar Box – Mulga – Coolibah Woodland and 16) Black Box Woodland. In total 355 vascular plant taxa were found of which 6% were considered exotic in origin. A population of Dentella minutissima; a species listed as threatened (endangered) under the New South Wales Threatened Species Conservation Act 1995 was found. A summary of select structural and habitat attributes within Alliances is also presented.
The shrub Nematolepis ovatifolia (F. Muell.) Paul G. Wilson (family Rutaceae) is endemic to the alpine and subalpine areas of the Snowy Mountains, Australia, where it dominates large areas of heath. Mass dieback was observed in the spring/summer of 2012. Damage at first was confined to the tips of branches, a symptom that could be due to frost damage and/or pathogen-induced water stress. Subsequently, whole stems and shrubs died and new areas of chlorosis appeared on smaller shrubs. Surveys of 186 sites covering the geographical range of the shrub in the summers of 2013/14 and 2014/15 found that 59 populations were definitely dieback affected, 92 had early symptoms and 35 were healthy. Two possible causes were investigated: killing frost and pathogens, with insect attack being a further cause of defoliation. The root rot pathogen, Phytophthora cambivora was isolated from one washed root sample and from one of five soil/ root samples. In 2014/15, in five sites where symptomatic plants were monitored, most plants recovered to a condition where they were considered unaffected in March 2015. It is possible that symptomatic plants had in fact suffered frost damage. Hence populations with early symptoms were grouped with healthy populations for analysis of proximity to trails. Compared with these, dieback affected populations were significantly closer to trails. It could not be determined when the pathogen was introduced, as it could have been imported on earthmoving equipment or by subsequent users of trails. It was apparently well spread before spring of 2012 but infection of plants was not evident. Its sudden eruption in spring of 2012 may have been facilitated by two warm and wet La Nina years, with mean growing season soil temperature up to 1.5°C higher than the long term mean and growing season rainfall double the long term mean. Plant death occurred in the hottest year on record in Australia, with the average alpine treeline growing season soil temperature of 9.2°C in 2012/13 being >2.0°C above the long term mean.
The recently described shrub Epacris browniae (family Ericaceae) an endemic Blue Mountains species, occurs on treeless, dry, rocky, Sydney Montane Heath, on Narrabeen and Hawkesbury Sandstone in the upper Blue Mountains, 100 km west of Sydney, New South Wales, (c. 33° 40' S; 150° 22' E) at altitudes above 800 m, and within the 1300 mm Average Annual Rainfall isohyet. Based on our surveys in the Blue Mountains (incorporating 46 locations), the Area of Occurrence of the species is estimated at 525 square km, within which the Area of Occupation is 25 hectares. The northern limit is Mt Wilson, southern limit, Mt Solitary, western limit appears to lie between Clarence and Narrow Neck (Katoomba) and eastern limit is Lawson Ridge.
The species grows either on flat terrain or on gentle southern or western slopes, sometimes blending into escarpment complex, on soils of moderate acidity (pH 6.5–6.8) and good drainage, associated most commonly with Banksia ericifolia and, in descending order of abundance, with Leptospermum trinervium, Allocasuarina distyla, Kunzea capitata, Allocasuarina nana and Hakea dactyloides.
Epacris browniae flowers in November and seeds ripen in March. Seeds are shiny, brown, minutely warty, about 0.6 mm long, and weigh 0.038 mg.
Epacris browniae does not resprout after fire. Average stem diameters at three sites with known single fire histories correlate strongly with likely maximum age and confirm that the species is a facultative reseeder with a potential lifespan of up to 50 years.
Despite its relatively narrow habitat and area of occupancy Epacris browniae is well conserved within National Parks in the Blue Mountains but its susceptibility to Phytophthora, an exotic pathogen in the Blue Mountains is unknown.