Katherine E. Sloan, Matthias Leisegang, Carmen Döbele, Ana S. Ramírez, Stefan Simm, Charlotta Safferthal, Jens Kretschmer, Tobias Schorge, Stavroula Markoutsa, Sara Haag, Michael Karas, Ingo Ebersberger, Enrico Schleiff, Nicholas J. Watkins, Markus Bohnsack
- Translation fidelity and efficiency require multiple ribosomal (r)RNA modifications that are mostly mediated by small nucleolar (sno)RNPs during ribosome production. Overlapping basepairing of snoRNAs with pre-rRNAs often necessitates sequential and efficient association and dissociation of the snoRNPs, however, how such hierarchy is established has remained unknown so far. Here, we identify several late-acting snoRNAs that bind pre-40S particles in human cells and show that their association and function in pre-40S complexes is regulated by the RNA helicase DDX21. We map DDX21 crosslinking sites on pre-rRNAs and show their overlap with the basepairing sites of the affected snoRNAs. While DDX21 activity is required for recruitment of the late-acting snoRNAs SNORD56 and SNORD68, earlier snoRNAs are not affected by DDX21 depletion. Together, these observations provide an understanding of the timing and ordered hierarchy of snoRNP action in pre-40S maturation and reveal a novel mode of regulation of snoRNP function by an RNA helicase in human cells.
MetadatenAuthor: | Katherine E. Sloan, Matthias LeisegangORCiDGND, Carmen Döbele, Ana S. Ramírez, Stefan SimmORCiDGND, Charlotta Safferthal, Jens Kretschmer, Tobias Schorge, Stavroula Markoutsa, Sara Haag, Michael KarasGND, Ingo EbersbergerORCiDGND, Enrico SchleiffORCiDGND, Nicholas J. Watkins, Markus BohnsackORCiDGND |
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URN: | urn:nbn:de:hebis:30:3-372194 |
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DOI: | https://doi.org/10.1093/nar/gku1291 |
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ISSN: | 1362-4962 |
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ISSN: | 0305-1048 |
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Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/25477391 |
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Parent Title (English): | Nucleic Acids Research |
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Publisher: | Oxford Univ. Press |
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Place of publication: | Oxford |
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Document Type: | Article |
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Language: | English |
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Date of Publication (online): | 2014/12/04 |
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Date of first Publication: | 2014/12/04 |
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Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
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Release Date: | 2015/03/30 |
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Volume: | 43 |
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Issue: | 1 |
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Page Number: | 12 |
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First Page: | 553 |
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Last Page: | 564 |
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Note: | © The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
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HeBIS-PPN: | 368983323 |
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Institutes: | Biochemie, Chemie und Pharmazie / Biochemie und Chemie |
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| Biowissenschaften / Biowissenschaften |
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| Exzellenzcluster / Exzellenzcluster Makromolekulare Komplexe |
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Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
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Sammlungen: | Universitätspublikationen |
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| Sammlung Biologie / Sondersammelgebiets-Volltexte |
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Licence (English): | Creative Commons - Namensnennung-Nicht kommerziell 4.0 |
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