Jennifer Vögele, Jan Ferner, Nadide Altınçekiç, Jasleen Kaur Bains, Betül Ceylan, Boris Fürtig, J. Tassilo Grün, Martin Hengesbach, Katharina Felicitas Hohmann, Daniel Hymon, Božana Knežić, Frank Löhr, Stephen A. Peter, Dennis Joshua Pyper, Nusrat Qureshi, Christian Richter, Andreas Schlundt, Harald Schwalbe, Elke Stirnal, Alexey Sudakov, Anna Wacker, Julia Weigand, Julia Wirmer-Bartoschek, Jens Wöhnert, Elke Duchardt-Ferner
- The SARS-CoV-2 virus is the cause of the respiratory disease COVID-19. As of today, therapeutic interventions in severe COVID-19 cases are still not available as no effective therapeutics have been developed so far. Despite the ongoing development of a number of effective vaccines, therapeutics to fight the disease once it has been contracted will still be required. Promising targets for the development of antiviral agents against SARS-CoV-2 can be found in the viral RNA genome. The 5′- and 3′-genomic ends of the 30 kb SCoV-2 genome are highly conserved among Betacoronaviruses and contain structured RNA elements involved in the translation and replication of the viral genome. The 40 nucleotides (nt) long highly conserved stem-loop 4 (5_SL4) is located within the 5′-untranslated region (5′-UTR) important for viral replication. 5_SL4 features an extended stem structure disrupted by several pyrimidine mismatches and is capped by a pentaloop. Here, we report extensive 1H, 13C, 15N and 31P resonance assignments of 5_SL4 as the basis for in-depth structural and ligand screening studies by solution NMR spectroscopy.
MetadatenAuthor: | Jennifer VögeleORCiD, Jan FernerORCiDGND, Nadide AltınçekiçORCiDGND, Jasleen Kaur BainsORCiDGND, Betül CeylanGND, Boris FürtigORCiDGND, J. Tassilo GrünORCiDGND, Martin HengesbachORCiDGND, Katharina Felicitas HohmannGND, Daniel Hymon, Božana KnežićGND, Frank LöhrORCiD, Stephen A. PeterGND, Dennis Joshua Pyper, Nusrat QureshiORCiDGND, Christian RichterORCiDGND, Andreas SchlundtORCiDGND, Harald SchwalbeORCiDGND, Elke Stirnal, Alexey Sudakov, Anna WackerORCiDGND, Julia WeigandORCiDGND, Julia Wirmer-BartoschekORCiD, Jens WöhnertORCiDGND, Elke Duchardt-FernerORCiD |
---|
URN: | urn:nbn:de:hebis:30:3-636594 |
---|
DOI: | https://doi.org/10.1007/s12104-021-10026-7 |
---|
ISSN: | 1874-270X |
---|
Parent Title (English): | Biomolecular NMR assignments |
---|
Publisher: | Springer Netherlands |
---|
Place of publication: | Dordrecht [u.a.] |
---|
Document Type: | Article |
---|
Language: | English |
---|
Date of Publication (online): | 2021/04/29 |
---|
Date of first Publication: | 2021/04/29 |
---|
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
---|
Release Date: | 2022/07/05 |
---|
Tag: | 5_SL4; 5′-UTR; COVID19-NMR; RNA genome; SARS-CoV-2; Solution NMR spectroscopy |
---|
Volume: | 15.2021 |
---|
Issue: | 2 |
---|
Page Number: | 6 |
---|
First Page: | 335 |
---|
Last Page: | 340 |
---|
Note: | Work at the Center for Biomolecular Magnetic Resonance (BMRZ) at the Goethe-University Frankfurt is supported by the state of Hesse. Work in Covid19-nmr was supported by the Goethe Corona Funds, by the IWB-EFRE-programme 20007375 of the state of Hesse, and the DFG in CRC902: “Molecular Principles of RNA-based regulation.” and infrastructure funds (Project Numbers: 277478796, 277479031, 392682309, 452632086, 70653611). H.S. and B.F. are supported by the DFG in graduate school CLIC (GRK 1986). |
---|
Note: | Open Access funding enabled and organized by Projekt DEAL. |
---|
HeBIS-PPN: | 497224534 |
---|
Institutes: | Biowissenschaften |
---|
| Wissenschaftliche Zentren und koordinierte Programme / Zentrum für Biomolekulare Magnetische Resonanz (BMRZ) |
---|
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
---|
| 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
---|
| 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
---|
Sammlungen: | Universitätspublikationen |
---|
Licence (German): | Creative Commons - Namensnennung 4.0 |
---|