Upgrading a consumer stereolithographic 3d printer to produce a physiologically relevant model with human liver cancer organoids

  • A widespread application of 3D bioprinting in basic and translational research requires accessibility to affordable printers able to produce physiologically relevant tissue models. To facilitate the use of bioprinting as a standard technique in biology, an open-source device based on a consumer-grade 3D stereolithography apparatus (SLA) printer is developed. This SLA bioprinter can produce complex constructs that preserve cell viability and recapitulate the physiology of tissues. The detailed documentation of the modifications apported to the printer as well as a throughout performance analysis allow for a straightforward adoption of the device in other labs and its customization for specific applications. Given the low cost, several modified bioprinters could be simultaneously operated for a parallelized tissue production. To showcase the capability of the bioprinter, constructs consisting of patient-derived cholangiocarcinoma organoids encapsulated in a gelatin methacrylate (GelMA)/polyethylene glycol diacrylate (PEGDA) hydrogel are produced. A thorough characterization of different GelMA/PEGDA ratios reveals that the mechanical properties of the bioprinted tumor model can be accurately fine-tuned to mimic a specific tumor micro-environment. Immunofluorescence and gene expression analyses of tumor markers confirm that the bioprinted synthetic hydrogel provides a flexible and adequate replacement of animal-derived reconstituted extracellular matrix.

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Author:Louise BreidebandORCiD, Kaja N. Wächtershäuser, Levin HafaORCiD, Konstantin Wieland, Achilleas S. FrangakisORCiDGND, Ernst H. K. StelzerORCiDGND, Francesco PampaloniORCiDGND
Parent Title (English):Advanced Materials Technologies
Place of publication:Weinheim
Document Type:Article
Date of Publication (online):2022/05/25
Date of first Publication:2022/05/25
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2023/10/18
Tag:additive manufacturing; liver cancer; open-source 3D bioprinting; organoids; stereolithography; tumor microenvironment; tumor model
Issue:10, art. 2200029
Article Number:2200029
Page Number:18
First Page:1
Last Page:18
Open access funding enabled and organized by Projekt DEAL.
The data that support the findings of this study are openly available in BioRxiv at https://doi.org/10.1101/2021.12.30.474546, reference number 474546.
Fachübergreifende Einrichtungen / Buchmann Institut für Molekulare Lebenswissenschaften (BMLS)
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
6 Technik, Medizin, angewandte Wissenschaften / 60 Technik / 600 Technik, Technologie
Licence (German):License LogoCreative Commons - CC BY - Namensnennung 4.0 International