1H, 13C, and 15N backbone chemical shift assignments of coronavirus-2 non-structural protein Nsp10

  • The international Covid19-NMR consortium aims at the comprehensive spectroscopic characterization of SARS-CoV-2 RNA elements and proteins and will provide NMR chemical shift assignments of the molecular components of this virus. The SARS-CoV-2 genome encodes approximately 30 different proteins. Four of these proteins are involved in forming the viral envelope or in the packaging of the RNA genome and are therefore called structural proteins. The other proteins fulfill a variety of functions during the viral life cycle and comprise the so-called non-structural proteins (nsps). Here, we report the near-complete NMR resonance assignment for the backbone chemical shifts of the non-structural protein 10 (nsp10). Nsp10 is part of the viral replication-transcription complex (RTC). It aids in synthesizing and modifying the genomic and subgenomic RNAs. Via its interaction with nsp14, it ensures transcriptional fidelity of the RNA-dependent RNA polymerase, and through its stimulation of the methyltransferase activity of nsp16, it aids in synthesizing the RNA cap structures which protect the viral RNAs from being recognized by the innate immune system. Both of these functions can be potentially targeted by drugs. Our data will aid in performing additional NMR-based characterizations, and provide a basis for the identification of possible small molecule ligands interfering with nsp10 exerting its essential role in viral replication.

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Author:Nina KubatovaORCiDGND, Nusrat QureshiORCiDGND, Nadide AltınçekiçORCiDGND, Rupert AbeleORCiDGND, Jasleen Kaur BainsORCiDGND, Betül Ceylan, Jan FernerORCiDGND, Christin FuksGND, Bruno Hargittay, Marie Hutchison, Vanessa de JesusORCiDGND, Felicitas Kutz, Maria Alexandra Wirtz MartinORCiD, Nathalie MeiserORCiDGND, Verena LinhardORCiD, Dennis Joshua Pyper, Sven TrucksGND, Boris FürtigORCiDGND, Martin HengesbachORCiDGND, Frank LöhrORCiD, Christian RichterORCiDGND, Krishna SaxenaORCiDGND, Andreas SchlundtORCiDGND, Harald SchwalbeORCiDGND, Sridhar SreeramuluGND, Anna WackerORCiDGND, Julia WeigandORCiDGND, Julia Wirmer-BartoschekORCiD, Jens WöhnertORCiDGND
Parent Title (English):Biomolecular NMR assignments
Publisher:Springer Netherlands
Place of publication:Dordrecht [u.a.]
Document Type:Article
Date of Publication (online):2020/11/07
Date of first Publication:2020/11/07
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2023/11/28
Tag:Covid19-NMR; Non-structural protein; SARS-CoV-2; Solution NMR-spectroscopy
Page Number:7
First Page:65
Last Page:71
Work at BMRZ is supported by the state of Hesse. Work in Covid19-NMR was supported by the Goethe Corona Funds, and by the DFG within the SFB902.
Open Access funding enabled and organized by Projekt DEAL.
Institutes:Biochemie, Chemie und Pharmazie
Wissenschaftliche Zentren und koordinierte Programme / Zentrum für Biomolekulare Magnetische Resonanz (BMRZ)
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften
5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Licence (German):License LogoCreative Commons - CC BY - Namensnennung 4.0 International