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Redirection of the transcription factor SP1 to AT rich binding sites by a synthetic adaptor molecule
- The ubiquitous transcription factor SP1 binds to a GC rich consensus sequence. Here we describe an adaptor molecule that mediates binding of SP1 to a non-cognate DNA site rich in AT. The adaptor is comprised of a Dervan-type hairpin polyamide with high affinity to an AT rich hexamer duplex. It also carries a 27mer DNA that contains the SP1 consensus sequence. The synthesis and purification of the polyamide-DNA conjugate is reported. Pulldown experiments and western blot analysis demonstrate adaptor mediated binding of SP1 to the hexamer duplex TTGTTA.
Author: | Mathias Bolz, Ute Scheffer, Elisabeth Kalden, Michael GöbelORCiDGND |
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URN: | urn:nbn:de:hebis:30:3-626529 |
DOI: | https://doi.org/10.1002/hlca.202100095 |
ISSN: | 1522-2675 |
Parent Title (English): | Helvetica chimica acta |
Publisher: | Wiley-VCH |
Place of publication: | New York, NY |
Document Type: | Article |
Language: | English |
Date of Publication (online): | 2021/08/08 |
Date of first Publication: | 2021/08/08 |
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Release Date: | 2021/12/20 |
Tag: | DNA conjugate; DNA recognition; adaptor mediated recruitment; hairpin polyamides; protein capture |
Volume: | 104 |
Issue: | 9, art. e2100095 |
Page Number: | 9 |
First Page: | 1 |
Last Page: | 9 |
Note: | Open access funding enabled and organized by Projekt DEAL. |
HeBIS-PPN: | 491320094 |
Institutes: | Biochemie, Chemie und Pharmazie |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie | |
Sammlungen: | Universitätspublikationen |
Licence (German): | Creative Commons - Namensnennung 4.0 |