1H, 13C and 15N assignment of stem-loop SL1 from the 5'-UTR of SARS-CoV-2

  • The stem-loop (SL1) is the 5'-terminal structural element within the single-stranded SARS-CoV-2 RNA genome. It is formed by nucleotides 7–33 and consists of two short helical segments interrupted by an asymmetric internal loop. This architecture is conserved among Betacoronaviruses. SL1 is present in genomic SARS-CoV-2 RNA as well as in all subgenomic mRNA species produced by the virus during replication, thus representing a ubiquitous cis-regulatory RNA with potential functions at all stages of the viral life cycle. We present here the 1H, 13C and 15N chemical shift assignment of the 29 nucleotides-RNA construct 5_SL1, which denotes the native 27mer SL1 stabilized by an additional terminal G-C base-pair.
Author:Christian RichterORCiDGND, Katharina Felicitas HohmannGND, Sabrina Toews, Daniel Mathieu, Nadide AltınçekiçORCiDGND, Jasleen Kaur BainsORCiDGND, Oliver BinasORCiDGND, Betül Ceylan, Elke Duchardt-FernerORCiD, Jan FernerORCiDGND, Boris FürtigORCiDGND, J. Tassilo GrünORCiDGND, Martin HengesbachORCiDGND, Daniel Hymon, Hendrik R. A. JonkerORCiD, Božana KnežićGND, Sophie KornORCiDGND, Tom LandgrafORCiDGND, Frank LöhrORCiD, Stephen A. PeterGND, Dennis Joshua Pyper, Nusrat QureshiORCiDGND, Andreas SchlundtORCiDGND, Robbin SchniedersGND, Elke Stirnal, Alexey Sudakov, Jennifer VögeleORCiD, Julia WeigandORCiDGND, Julia Wirmer-BartoschekORCiD, Kerstin Witt, Jens WöhnertORCiDGND, Harald SchwalbeORCiDGND, Anna WackerORCiDGND
Parent Title (English):Biomolecular NMR assignments
Publisher:Springer Netherlands
Place of publication:Dordrecht [u.a.]
Document Type:Article
Date of Publication (online):2021/08/28
Date of first Publication:2021/08/28
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2022/03/09
Tag:5'-UTR; COVID19-NMR; SARS-CoV-2; SL1; Solution NMR spectroscopy
Page Number:8
First Page:467
Last Page:474
Open Access funding enabled and organized by Projekt DEAL. Work at the Center for Biomolecular Magnetic Resonance (BMRZ) at the Goethe-University Frankfurt is supported by the state of Hesse. Work in Covid19-nmr was supported by the Goethe Corona Funds, by the IWB-EFRE-programme 20007375 of the state of Hesse, and the DFG in CRC902: “Molecular Principles of RNA-based regulation.” and infrastructure funds (Project Numbers: 277478796, 277479031, 392682309, 452632086, 70653611). K. F. H., B. F., B. K., H. S., and J. T. G. are supported by the DFG in graduate school CLIC (GRK 1986). D. J. P. and H. S. are supported by the DFG in SPP2002. A.W., B. F., O. B., S. K., A. S., M. H., Ju. Wei, Je. Woe. and H. S. are supported by the DFG in CRC902. T. L. and H. S. are supported by the DFG in SPP1879. J. K. B. and H. S. are supported by the DFG in TRR 267, H. R. A. Jonker and H.S. are supported by the DFG in FOR2509. A. S. is funded by the Deutsche Forschungsgemeinschaft through Grant number SCHL2062/2-1 and by the Johanna Quandt Young Academy at Goethe (Grant number 2019/AS01).
Institutes:Biochemie, Chemie und Pharmazie
Wissenschaftliche Zentren und koordinierte Programme / Zentrum für Biomolekulare Magnetische Resonanz (BMRZ)
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften
5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Licence (German):License LogoCreative Commons - Namensnennung 4.0