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Mohamed Samy Abdallah, Bassam Aboona, Jaroslav Adam, Leszek Adamczyk, Joseph R. Adams, J. Kevin Adkins, Ishu Aggarwal, Madan Mohan Aggarwal, Zubayer Ahammed, Derek M. Anderson, Elke Caroline Aschenauer, Muhammad Usman Ashraf, J. Atchison, Vipul Bairathi, W. Baker, Jonathan Gonzalo Ball Cap, Kenneth Barish, Arabinda Behera, Rene Bellwied, Pratibha Bhagat, Anju Bhasin, Jaroslav Bielčík, Jana Bielčíková, James D. Brandenburg, Xiang-Zhou Cai, Helen Louise Caines, Manuel Calderón de la Barca Sánchez, Daniel A. Cebra, Irakli Chakaberia, Petr Chaloupka, B. K. Chan, Z. Chang, A. Chatterjee, S. Chattopadhyay, D. Chen, J. Chen, J. H. Chen, X. Chen, Z. Chen, J. Cheng, Subikash Choudhury, William Christie, Xiaoxuan Chu, Henry J. Crawford, Máté Csanád, M. Daugherity, Ingo Martin Deppner, A. Dhamija, L. Di Carlo, Lidia Didenko, Prabhupada Dixit, X. Dong, James Lucus Drachenberg, Emilie Duckworth, James C. Dunlop, Jon M. Engelage, Geary Wareham Eppley, ShinIchi Esumi, Olga Y. Evdokimov, A. 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• Measurements of mass and Λ binding energy of 4ΛH and 4ΛHe in Au+Au collisions at sNN−−−√=3 GeV are presented, with an aim to address the charge symmetry breaking (CSB) problem in hypernuclei systems with atomic number A = 4. The Λ binding energies are measured to be 2.22±0.06(stat.)±0.14(syst.) MeV and 2.38±0.13(stat.)±0.12(syst.) MeV for 4ΛH and 4ΛHe, respectively. The measured Λ binding-energy difference is 0.16±0.14(stat.)±0.10(syst.) MeV for ground states. Combined with the γ-ray transition energies, the binding-energy difference for excited states is −0.16±0.14(stat.)±0.10(syst.) MeV, which is negative and comparable to the value of the ground states within uncertainties. These new measurements on the Λ binding-energy difference in A = 4 hypernuclei systems are consistent with the theoretical calculations that result in ΔB4Λ(1+exc)≈−ΔB4Λ(0+g.s.)<0 and present a new method for the study of CSB effect using relativistic heavy-ion collisions.